Detailed information NTG0944741

Bioinformatically predicted

Overview

• Name: hexA
• Type: Machinery gene
• Locus tag: ACFW99_RS08780
• Genome accession: NZ_CP170651
• Coordinates: 1750102..1752687 (+)
• Length: 861 a.a.
• NCBI ID: WP_033574833.1
• Uniprot ID: -
• Organism: Bacillus amyloliquefaciens strain JP5025
• Function: DNA mismatch repair (predicted from homology) Homologous recombination

Genomic Context

Location: 1745102..1757687

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACFW99_RS08760 (ACFW99_08760)	-	1745899..1747077 (+)	1179	WP_029325912.1	glycine C-acetyltransferase	-
ACFW99_RS08765 (ACFW99_08765)	miaB	1747220..1748749 (+)	1530	WP_015417500.1	tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB	-
ACFW99_RS08770 (ACFW99_08770)	ymcA	1748752..1749183 (+)	432	WP_003154113.1	RicAFT regulatory complex protein RicA family protein	Regulator
ACFW99_RS08775 (ACFW99_08775)	cotE	1749438..1749983 (+)	546	WP_003154111.1	outer spore coat protein CotE	-
ACFW99_RS08780 (ACFW99_08780)	hexA	1750102..1752687 (+)	2586	WP_033574833.1	DNA mismatch repair protein MutS	Machinery gene
ACFW99_RS08785 (ACFW99_08785)	hexB	1752703..1754577 (+)	1875	WP_192452986.1	DNA mismatch repair endonuclease MutL	Machinery gene
ACFW99_RS08790 (ACFW99_08790)	-	1754773..1755246 (+)	474	WP_060675479.1	tyrosine-type recombinase/integrase	-
ACFW99_RS08795 (ACFW99_08795)	-	1755707..1756345 (+)	639	WP_060675480.1	hypothetical protein	-
ACFW99_RS08800 (ACFW99_08800)	-	1756421..1756786 (+)	366	WP_060675481.1	HNH endonuclease	-

Sequence

Protein

• Length: 861 a.a.
• Molecular weight: 97753.93 Da
• Isoelectric Point: 5.3034

>NTDB_id=944741 ACFW99_RS08780 WP_033574833.1 1750102..1752687(+) (hexA) [Bacillus amyloliquefaciens strain JP5025]
MASYTPMIQQYLKIKAEHQDAFLFFRLGDFYEMFFEDAKKASQELEITLTSRDGGSADKIPMCGVPYHSAAAYIEQLIKK
GYKVAICEQTEDPKAAKGVVKREVVQLITPGTVMDGKGIHESENNFIASVSEFRDGYGLALSDLTTGENLAVFIERIEDV
MSEIYSVGAKEIVVSSKFNEHTAAQLKERCGATISIEDGEITERIEIAKHLPGEELTETFMRLYTYLQKTQKRSLDHLQP
VQVYELEEAMKIDLYSKRNLELTETIRSKSKKGSLLWLLDETKTAMGGRLLKQWIDRPLIRASQIEERQEMVETLINHLF
EREDLRERLKEVYDLERLAGRVAFGNVNARDLIQLKESLKQVPSIKELVGSLNHKKAKERAGLIDPCGDLLDLLEDALHE
NPPLSLKEGNLIKDGYHQKLDEYRDASKNGKDWIARLEQQERAYTGIRSLKVGFNKVFGYYIEVTKANLHLLEDGRYERK
QTLTNAERYITPELKEKEALILEAENNICELEYELFAGLREKVKQFIPRLQRLAKQMSELDALQCFATISENRHYTKPVF
SDNEVKVIEGRHPVVEKVMDSQEYVPNNCLMGDSREMLLITGPNMSGKSTYMRQIALLSIMAQIGCFVPAKEAVLPIFDQ
IFTRIGAADDLISGQSTFMVEMLEAKNAIVNATKDSLILFDEIGRGTSTYDGMALAQAIIEYVHDHIGAKTLFSTHYHEL
TVLEDKLPQLKNVHVRAEEYNGTVVFLHQIKEGAADKSYGIHVAQLAELPDDLISRAQEILKQLEQTGDKPELPAVSEKK
SAVREEPAQLSFFTDGEKEQKAPAVSNKEKQVLEAFKSINILDMTPLEAMNEMYKLQKKLK

Nucleotide

• Length: 2586 bp

>NTDB_id=944741 ACFW99_RS08780 WP_033574833.1 1750102..1752687(+) (hexA) [Bacillus amyloliquefaciens strain JP5025]
ATGGCTAGTTACACGCCTATGATACAGCAATACTTGAAGATAAAGGCAGAGCACCAAGATGCCTTTTTATTTTTTCGGCT
TGGTGACTTTTATGAAATGTTTTTTGAAGATGCAAAAAAAGCGTCACAAGAGCTTGAAATTACACTGACAAGCAGAGACG
GAGGTTCGGCTGATAAAATTCCGATGTGCGGCGTGCCTTATCATTCCGCGGCGGCTTATATTGAACAGCTGATTAAAAAG
GGCTATAAAGTGGCGATCTGTGAACAGACGGAAGATCCGAAGGCGGCTAAAGGCGTTGTAAAAAGAGAAGTCGTCCAGCT
GATCACACCGGGAACCGTGATGGATGGAAAAGGCATCCACGAGTCGGAAAACAACTTTATCGCTTCCGTTTCCGAATTTC
GTGACGGATACGGTCTCGCTTTATCGGACTTAACGACCGGAGAAAACCTGGCCGTATTTATTGAGCGGATTGAAGATGTC
ATGTCAGAAATTTACTCAGTCGGCGCAAAGGAAATTGTCGTATCGAGCAAGTTTAACGAACATACAGCTGCTCAGCTGAA
AGAACGGTGCGGCGCCACGATTTCCATCGAAGACGGGGAAATAACAGAACGCATTGAAATCGCAAAGCACTTGCCGGGCG
AAGAGCTGACAGAGACGTTTATGCGTTTATATACGTATTTGCAAAAAACGCAAAAACGGAGCCTGGATCACCTGCAGCCG
GTTCAAGTATATGAATTGGAAGAAGCGATGAAAATTGACCTGTATTCAAAACGGAATCTTGAGCTGACGGAAACGATCCG
TTCGAAAAGCAAAAAAGGGTCATTGCTTTGGCTTTTAGACGAGACGAAAACAGCGATGGGCGGCCGGCTTCTCAAACAAT
GGATTGACCGCCCGCTGATCAGGGCATCCCAAATCGAAGAACGCCAGGAAATGGTCGAGACGCTGATCAATCATCTGTTT
GAGCGTGAAGATCTGCGAGAGCGCCTGAAGGAAGTATACGATTTAGAACGTCTGGCGGGCCGGGTCGCATTCGGAAACGT
CAACGCAAGGGATTTAATTCAGCTGAAAGAATCTTTAAAACAAGTGCCGAGCATCAAAGAGCTTGTAGGGTCTCTTAACC
ATAAAAAAGCAAAAGAACGCGCCGGCTTGATTGATCCGTGCGGAGATTTGCTTGACCTGTTAGAAGATGCCCTTCATGAA
AACCCGCCGTTATCGTTAAAAGAAGGCAATTTAATTAAAGACGGCTATCATCAGAAGCTCGATGAGTACCGTGACGCCAG
CAAAAACGGTAAAGACTGGATTGCCAGACTGGAGCAGCAGGAACGCGCTTATACGGGCATCCGTTCATTAAAGGTCGGTT
TCAATAAAGTGTTCGGCTATTATATTGAAGTCACAAAAGCAAACCTTCATCTGCTTGAAGACGGACGGTATGAGCGGAAG
CAGACCCTCACAAACGCCGAACGGTACATTACGCCGGAATTGAAGGAAAAAGAAGCGCTTATTTTAGAAGCGGAAAACAA
CATCTGCGAGCTGGAATATGAGCTTTTTGCCGGACTGCGTGAAAAGGTGAAACAATTTATTCCGCGCTTGCAGCGTCTTG
CCAAACAAATGAGTGAGCTGGACGCACTGCAATGCTTTGCTACGATCAGTGAAAACAGGCATTACACAAAGCCCGTCTTT
TCTGATAATGAAGTAAAAGTCATTGAAGGGAGACACCCCGTCGTTGAAAAGGTGATGGACAGTCAAGAATACGTGCCGAA
CAACTGCCTGATGGGCGATTCGCGGGAAATGCTCCTGATTACGGGTCCGAATATGTCAGGTAAAAGCACGTATATGAGAC
AGATCGCACTTCTATCGATCATGGCTCAGATCGGCTGTTTTGTGCCCGCAAAAGAAGCGGTTCTGCCGATTTTTGATCAA
ATCTTCACGAGAATCGGGGCAGCGGATGATTTGATTTCCGGACAAAGCACCTTTATGGTGGAAATGCTGGAAGCGAAAAA
CGCGATTGTGAACGCCACAAAAGACAGCCTGATCCTGTTTGATGAAATCGGCCGCGGGACATCAACGTACGACGGAATGG
CGCTGGCACAGGCGATTATTGAGTATGTCCATGATCATATCGGCGCTAAAACACTGTTCAGCACGCATTATCATGAGCTG
ACGGTGCTTGAAGATAAGCTTCCTCAGCTGAAAAACGTTCATGTGCGCGCAGAGGAATATAACGGAACGGTCGTGTTTCT
CCATCAAATCAAAGAAGGAGCCGCAGATAAAAGTTACGGCATTCATGTCGCCCAGCTTGCCGAGCTCCCGGACGATCTGA
TCAGCCGCGCGCAGGAGATTTTGAAACAGCTTGAGCAGACGGGAGATAAACCGGAGCTTCCGGCGGTATCTGAGAAAAAG
TCCGCCGTCAGAGAAGAACCGGCACAGCTGTCCTTTTTTACTGACGGTGAAAAAGAACAGAAGGCACCGGCTGTTTCTAA
TAAAGAAAAACAAGTGCTTGAAGCTTTTAAGTCAATTAATATTCTCGATATGACGCCGCTTGAGGCGATGAATGAAATGT
ACAAGCTGCAAAAAAAATTAAAATAG

Domains

Predicted by InterProScan.

• MutS domain III (MutS_III) (258-547)

• MutS domain II (MutS_II) (125-239)

• MutS family domain IV (MutS_IV) (415-506)

• MutS domain V (MutS_V) (598-785)

• MutS domain I (MutS_I) (5-116)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
hexA	Streptococcus pneumoniae R6	48.191	99.535	0.48
mutS	Pseudomonas stutzeri strain ATCC 17587	38.322	100	0.393