Detailed information    

insolico Bioinformatically predicted

Overview

Name   stkP   Type   Regulator
Locus tag   ACFW99_RS08140 Genome accession   NZ_CP170651
Coordinates   1622026..1623969 (+) Length   647 a.a.
NCBI ID   WP_227051241.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain JP5025     
Function   require for competence development; phosphorylate ComE (predicted from homology)   
Competence regulation

Genomic Context

Location: 1617026..1628969
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFW99_RS08115 (ACFW99_08115) def 1617400..1617882 (+) 483 WP_033574585.1 peptide deformylase -
  ACFW99_RS08120 (ACFW99_08120) fmt 1617887..1618840 (+) 954 WP_012117531.1 methionyl-tRNA formyltransferase -
  ACFW99_RS08125 (ACFW99_08125) rsmB 1618827..1620170 (+) 1344 WP_061890591.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  ACFW99_RS08130 (ACFW99_08130) rlmN 1620174..1621265 (+) 1092 WP_003154339.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  ACFW99_RS08135 (ACFW99_08135) - 1621271..1622032 (+) 762 WP_007409750.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -
  ACFW99_RS08140 (ACFW99_08140) stkP 1622026..1623969 (+) 1944 WP_227051241.1 Stk1 family PASTA domain-containing Ser/Thr kinase Regulator
  ACFW99_RS08145 (ACFW99_08145) rsgA 1623984..1624874 (+) 891 WP_007409752.1 ribosome small subunit-dependent GTPase A -
  ACFW99_RS08150 (ACFW99_08150) rpe 1624876..1625526 (+) 651 WP_317859642.1 ribulose-phosphate 3-epimerase -
  ACFW99_RS08155 (ACFW99_08155) - 1625595..1626239 (+) 645 WP_061861452.1 thiamine diphosphokinase -
  ACFW99_RS08160 (ACFW99_08160) spoVM 1626326..1626406 (+) 81 WP_003154329.1 stage V sporulation protein SpoVM -
  ACFW99_RS08165 (ACFW99_08165) rpmB 1626484..1626672 (-) 189 WP_003154328.1 50S ribosomal protein L28 -
  ACFW99_RS08170 (ACFW99_08170) - 1626931..1627293 (+) 363 WP_003154327.1 Asp23/Gls24 family envelope stress response protein -

Sequence

Protein


Download         Length: 647 a.a.        Molecular weight: 71705.39 Da        Isoelectric Point: 5.1202

>NTDB_id=944731 ACFW99_RS08140 WP_227051241.1 1622026..1623969(+) (stkP) [Bacillus amyloliquefaciens strain JP5025]
MLTGKRISGRYHILRPIGGGGMANVFLAEDIILEREVAIKILRFDFVNDIDFIRRFRREAQSASSLDHPNIVSIYDIGEE
GDIYYIVMEYVEGMTLKEYINAHGPLHPKEALSVMEQIVSAIAHAHQNHIVHRDIKPHNILIDHLGHIKVTDFGIATALT
STTITHTNSVLGSVHYLSPEQARGGLATKKSDIYALGIVLFELLTGKIPFDGESAVSIALKHLQTETPSARKWNPSIPQS
VENIILKATAKDPFHRYESAEDMEADIRTAFDAGRLNEQKFSVQDDEEMTKAIPVITDGAKAAPQAETPGETDDKEQQPA
KKKKRKWPWVLLAVCFIFIMAAVLAVTVFPSLFMPKDVSVPDVRGMKYEKAEALLEKNGLRADPDITDIEDEKIEEELMV
KTDPKAGSTVKEGSSVKLYKSIGKPKTQLIDVKGRQIGDAKKALKEKGFKHVNVKEENDDSEAGTVIDQNPSAGTDMVAS
DDEVNLTVSLGPADVTLRDLKTYSKEAASGYLEDNGLQLVEKQAHSDDVPKGQVMKQEPAAGTAVKPGSNVEVTFSLGPE
EKPAKTVKEKISIPYEPEHEGDELEVQIAIDDKDHSISDTYDSFKIKEPTEKIIELKIDQGQKGYYQVMVDHKVVSYKTI
EYPKDND

Nucleotide


Download         Length: 1944 bp        

>NTDB_id=944731 ACFW99_RS08140 WP_227051241.1 1622026..1623969(+) (stkP) [Bacillus amyloliquefaciens strain JP5025]
GTGCTAACCGGCAAGCGGATCAGCGGGCGTTATCATATCCTCCGGCCCATTGGCGGCGGCGGAATGGCAAACGTTTTTTT
AGCTGAGGACATCATTCTCGAACGTGAAGTCGCAATTAAAATTCTGCGGTTTGACTTTGTGAATGACATTGATTTTATCA
GGCGTTTCAGAAGAGAAGCACAGTCGGCATCAAGCCTCGATCATCCGAATATCGTCAGTATTTACGATATCGGGGAGGAA
GGCGATATTTATTACATTGTCATGGAATATGTGGAAGGCATGACATTAAAGGAATACATAAACGCACACGGACCGCTTCA
TCCGAAAGAAGCGCTGTCTGTTATGGAGCAGATCGTCTCGGCCATCGCCCATGCCCATCAAAACCATATCGTGCACCGGG
ATATTAAACCGCACAATATATTGATAGATCATTTAGGGCATATCAAGGTGACGGATTTCGGCATCGCCACAGCGCTTACG
TCTACGACAATCACACATACGAATTCTGTGCTGGGCTCGGTTCATTATTTGTCTCCGGAGCAAGCGAGAGGCGGTCTGGC
AACGAAAAAATCAGATATTTACGCGCTCGGCATCGTGCTGTTTGAGCTTTTAACAGGTAAAATTCCTTTTGACGGAGAAT
CAGCCGTCAGCATAGCGCTAAAGCATCTGCAAACAGAAACGCCTTCCGCCAGAAAGTGGAACCCTTCCATTCCGCAGAGC
GTCGAAAACATTATTTTAAAGGCAACCGCTAAAGACCCGTTTCACCGCTATGAAAGCGCCGAGGACATGGAAGCGGACAT
CAGAACGGCTTTTGACGCCGGCAGGCTGAATGAACAAAAATTTTCCGTGCAGGATGATGAGGAAATGACAAAAGCCATTC
CCGTCATTACTGACGGCGCAAAAGCCGCGCCTCAGGCGGAAACGCCGGGGGAGACAGATGATAAGGAACAGCAGCCCGCC
AAAAAGAAAAAACGGAAATGGCCGTGGGTTTTGCTGGCCGTCTGTTTCATTTTCATTATGGCAGCTGTTCTTGCGGTCAC
CGTGTTCCCGTCTTTGTTTATGCCTAAAGATGTAAGCGTCCCCGACGTACGGGGCATGAAGTACGAAAAAGCGGAAGCCC
TGCTTGAAAAGAACGGGCTCCGGGCGGATCCTGATATAACGGATATTGAAGATGAAAAAATAGAAGAGGAATTAATGGTC
AAAACGGACCCAAAAGCGGGGTCTACCGTTAAAGAAGGCTCTTCTGTAAAACTGTACAAAAGCATCGGAAAACCGAAAAC
CCAGCTGATCGACGTGAAAGGGCGGCAGATCGGCGACGCAAAAAAAGCGCTGAAGGAAAAAGGCTTCAAACATGTCAATG
TCAAAGAAGAAAACGATGACAGCGAGGCCGGCACCGTAATTGATCAGAACCCCTCAGCCGGCACCGATATGGTCGCAAGC
GACGATGAAGTCAATCTAACGGTCAGCCTCGGCCCGGCGGACGTTACGCTGAGGGACTTAAAGACTTACAGCAAAGAAGC
GGCTTCAGGGTATCTTGAAGATAACGGCCTGCAGCTTGTTGAAAAACAAGCCCATTCAGATGACGTGCCAAAAGGACAAG
TCATGAAGCAAGAGCCGGCAGCCGGCACTGCGGTTAAGCCGGGAAGTAATGTTGAAGTGACGTTCTCGCTCGGACCTGAA
GAAAAACCGGCGAAAACGGTAAAAGAAAAAATCAGCATTCCTTACGAACCCGAACATGAAGGGGACGAGCTTGAAGTGCA
AATCGCCATCGATGATAAGGACCACAGCATCTCTGATACGTATGACTCATTTAAAATAAAAGAGCCGACTGAAAAAATAA
TTGAACTGAAGATTGATCAGGGACAGAAGGGGTATTATCAAGTGATGGTCGATCATAAAGTCGTCAGCTACAAAACGATT
GAATACCCGAAAGATAATGACTGA

Domains

Predicted by InterProScan.

PASTA domain (PASTA)

(495-556)

PASTA domain (PASTA)

(431-489)

PASTA domain (PASTA)

(358-419)

Protein kinase domain (Pkinase)

(11-260)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  stkP Streptococcus pneumoniae TIGR4

39.274

93.663

0.368
  stkP Streptococcus pneumoniae D39

39.109

93.663

0.366
  stkP Streptococcus pneumoniae R6

39.109

93.663

0.366
  stkP Streptococcus pneumoniae Rx1

38.944

93.663

0.365