NTDB

Overview

Name	comEA	Type	Machinery gene
Locus tag	V9W63_RS12190	Genome accession	NZ_CP146760
Coordinates	2523555..2524169 (-)	Length	204 a.a.
NCBI ID	WP_020956097.1	Uniprot ID	-
Organism	Bacillus velezensis strain JJ334
Function	dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2518555..2529169

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
V9W63_RS12160 (V9W63_12160)	rpsT	2518858..2519124 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
V9W63_RS12165 (V9W63_12165)	holA	2519141..2520182 (-)	1042	Protein_2348	DNA polymerase III subunit delta	-
V9W63_RS12170 (V9W63_12170)	-	2520222..2520373 (-)	152	Protein_2349	hypothetical protein	-
V9W63_RS12175 (V9W63_12175)	-	2520414..2520548 (+)	135	WP_003152870.1	YqzM family protein	-
V9W63_RS12180 (V9W63_12180)	comEC	2520567..2522918 (-)	2352	WP_060674655.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
V9W63_RS12185 (V9W63_12185)	-	2522919..2523488 (-)	570	WP_007408264.1	ComE operon protein 2	-
V9W63_RS12190 (V9W63_12190)	comEA	2523555..2524169 (-)	615	WP_020956097.1	helix-hairpin-helix domain-containing protein	Machinery gene
V9W63_RS12195 (V9W63_12195)	comER	2524228..2525049 (+)	822	WP_020956098.1	late competence protein ComER	-
V9W63_RS12200 (V9W63_12200)	-	2525118..2525855 (-)	738	WP_338683689.1	methyltransferase domain-containing protein	-
V9W63_RS12205 (V9W63_12205)	rsfS	2525852..2526208 (-)	357	WP_007408260.1	ribosome silencing factor	-
V9W63_RS12210 (V9W63_12210)	yqeK	2526226..2526786 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
V9W63_RS12215 (V9W63_12215)	-	2526776..2527345 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
V9W63_RS12220 (V9W63_12220)	yhbY	2527356..2527646 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
V9W63_RS12225 (V9W63_12225)	aroE	2527640..2528482 (-)	843	WP_144663968.1	shikimate dehydrogenase	-

Sequence

Protein

Download Length: 204 a.a. Molecular weight: 22251.45 Da Isoelectric Point: 6.4940

                                >NTDB_id=944321 V9W63_RS12190 WP_020956097.1 2523555..2524169(-) (comEA) [Bacillus velezensis strain JJ334]

MTKAMMTERLRRNLKILGACLVAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY

EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG

QKKAEAIIAYREEKGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

Download Length: 615 bp

                                >NTDB_id=944321 V9W63_RS12190 WP_020956097.1 2523555..2524169(-) (comEA) [Bacillus velezensis strain JJ334]

ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGTAGCAGGGGCGGTTTT

TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT

CCGTCGCGGACAAAAAAGACACCGCAGACGACAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT

GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT

TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG

CGGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG

CAGAAAAAAGCGGAAGCGATTATCGCTTACCGAGAAGAAAAAGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC

AGGCTTTGGAGAGAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(68-122)

Helix-hairpin-helix motif (HHH_3)

(141-202)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.115	93.627	0.544
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.071	100	0.363

Detailed information NTG0944321