Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEC   Type   Machinery gene
Locus tag   V9W63_RS12180 Genome accession   NZ_CP146760
Coordinates   2520567..2522918 (-) Length   783 a.a.
NCBI ID   WP_060674655.1    Uniprot ID   -
Organism   Bacillus velezensis strain JJ334     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2515567..2527918
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V9W63_RS12145 (V9W63_12145) - 2515932..2516270 (-) 339 WP_007408270.1 YqxA family protein -
  V9W63_RS12150 (V9W63_12150) - 2516287..2517480 (-) 1194 WP_007612673.1 stage II sporulation protein P -
  V9W63_RS12155 (V9W63_12155) gpr 2517549..2518655 (-) 1107 WP_338683687.1 GPR endopeptidase -
  V9W63_RS12160 (V9W63_12160) rpsT 2518858..2519124 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  V9W63_RS12165 (V9W63_12165) holA 2519141..2520182 (-) 1042 Protein_2348 DNA polymerase III subunit delta -
  V9W63_RS12170 (V9W63_12170) - 2520222..2520373 (-) 152 Protein_2349 hypothetical protein -
  V9W63_RS12175 (V9W63_12175) - 2520414..2520548 (+) 135 WP_003152870.1 YqzM family protein -
  V9W63_RS12180 (V9W63_12180) comEC 2520567..2522918 (-) 2352 WP_060674655.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  V9W63_RS12185 (V9W63_12185) - 2522919..2523488 (-) 570 WP_007408264.1 ComE operon protein 2 -
  V9W63_RS12190 (V9W63_12190) comEA 2523555..2524169 (-) 615 WP_020956097.1 helix-hairpin-helix domain-containing protein Machinery gene
  V9W63_RS12195 (V9W63_12195) comER 2524228..2525049 (+) 822 WP_020956098.1 late competence protein ComER -
  V9W63_RS12200 (V9W63_12200) - 2525118..2525855 (-) 738 WP_338683689.1 methyltransferase domain-containing protein -
  V9W63_RS12205 (V9W63_12205) rsfS 2525852..2526208 (-) 357 WP_007408260.1 ribosome silencing factor -
  V9W63_RS12210 (V9W63_12210) yqeK 2526226..2526786 (-) 561 WP_014418422.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  V9W63_RS12215 (V9W63_12215) - 2526776..2527345 (-) 570 WP_007408258.1 nicotinate-nucleotide adenylyltransferase -
  V9W63_RS12220 (V9W63_12220) yhbY 2527356..2527646 (-) 291 WP_003152858.1 ribosome assembly RNA-binding protein YhbY -

Sequence

Protein


Download         Length: 783 a.a.        Molecular weight: 86669.42 Da        Isoelectric Point: 8.7893

>NTDB_id=944319 V9W63_RS12180 WP_060674655.1 2520567..2522918(-) (comEC) [Bacillus velezensis strain JJ334]
MKYKYLLLPLAAVSATAGIAAAHVFWVLLLFLLYLLFIIVKTKHHAPVIVCLVSFCLYFYLYTVCDAANVTRYQAGSYTE
QAVITNIPKVDGAKMSAVIRTHDKEKWAASYKIRSLEEKRLIEQLEPGMRCTFTGSLEQPAHATVPGGFDYKEYLYSQQI
HWLFSVTSIEQCEKSKQPLFKLLNIRKNLISIIRNHVPESSAGIVEALTLGERFSIEDDILSAYQNLGVVHLMAISGMHV
GLITAGLFYALIRIGLTREKAGILLLLFLPVYTLLSGAAPSVLRASLMLGFYIAGTLVKRGIHSSAALSLSYLLLLLFNP
YFLWQAGFQLSFAVSASLILSSSILKKAGESRLAGLAMASFIAELSSLPFLLYHFQQISLVSFPMNMVMVPFYTLFVIPV
SVIGFLLLLLSRQMGECLFGMFDLVMKPVHDFITYAASVDLFTMIVSKPDFVSLLLLAVSVFTLFAALEKGGFLKLRKSA
LFFCAVLAYLICRPYFSPWGEADMLDIGQGDSLFISAPHRKGTVMVDTGGVIAYPGESWKEKRHPYSIGEKVLIPFLNGK
GVKKLDALILTHADQDHIGEAGVLIKNHRVKRLIVPVGFVKEPKDQNILNMAKENNIPVAEAKRGDTITAGDLQFQVLSP
ESSDGRSKNDSSLVLWTVFGGVSWLLTGDLESDGETEVLKTYPNLKADILKAGHHGSKSSTSEAFLKQLQPEAALISAGK
ENRYHHPHEEVLDRLKAYSVNVLRTDVSGTIQYRFKKGAGTFSVFPPYDIEETRAQEVKKTAD

Nucleotide


Download         Length: 2352 bp        

>NTDB_id=944319 V9W63_RS12180 WP_060674655.1 2520567..2522918(-) (comEC) [Bacillus velezensis strain JJ334]
ATGAAATATAAATACCTTCTTCTGCCTCTGGCGGCGGTTTCTGCAACTGCGGGAATTGCCGCTGCTCATGTCTTCTGGGT
TCTGCTCCTTTTTCTTCTGTATCTTCTCTTTATTATTGTAAAAACAAAGCACCATGCTCCGGTTATCGTCTGCCTCGTTT
CTTTTTGTCTTTATTTCTATCTTTATACGGTTTGTGACGCTGCGAATGTAACGCGCTATCAGGCCGGCAGTTATACTGAA
CAGGCCGTCATCACTAATATTCCGAAGGTTGACGGAGCGAAAATGTCAGCTGTTATCCGTACACATGACAAGGAAAAATG
GGCGGCTTCGTACAAAATCCGTTCTCTTGAGGAAAAAAGACTCATTGAACAGCTTGAACCGGGGATGCGCTGCACGTTTA
CAGGCTCTCTGGAACAGCCTGCACATGCGACGGTTCCCGGAGGTTTTGATTATAAGGAATATCTTTACTCTCAGCAGATT
CACTGGTTATTTTCCGTGACTTCCATTGAGCAGTGTGAAAAATCCAAACAGCCGCTGTTTAAACTGCTGAACATCAGAAA
AAATTTGATTTCGATCATTCGGAATCACGTGCCTGAATCTTCCGCCGGAATTGTTGAAGCGCTGACCTTAGGTGAAAGAT
TTTCTATAGAGGACGATATACTGAGTGCATATCAAAATTTGGGAGTCGTTCATTTAATGGCGATTTCCGGAATGCATGTC
GGTCTTATTACGGCGGGATTATTTTATGCTCTGATCAGAATCGGGCTGACAAGAGAAAAGGCGGGAATTTTGCTGCTGCT
GTTTTTGCCGGTGTATACGCTGCTGAGCGGTGCCGCCCCATCCGTATTGCGCGCATCCCTCATGCTGGGATTTTATATCG
CCGGAACTCTTGTTAAACGCGGCATTCATTCCTCTGCTGCATTGTCTTTGTCTTATCTGCTGCTTCTGCTGTTTAATCCT
TACTTCCTTTGGCAGGCGGGCTTCCAGCTTTCCTTTGCGGTAAGCGCCTCTTTAATTCTGTCATCCTCCATTTTAAAGAA
AGCAGGGGAAAGCAGACTTGCCGGGCTTGCGATGGCTTCATTCATTGCGGAGCTCAGCTCACTTCCGTTTCTTCTCTATC
ATTTTCAGCAGATTTCACTTGTCAGTTTTCCGATGAATATGGTGATGGTGCCTTTTTATACGTTATTTGTCATTCCGGTT
TCTGTCATCGGTTTCCTTCTTCTTTTACTCTCAAGGCAGATGGGAGAATGTTTGTTTGGTATGTTTGACCTTGTGATGAA
GCCTGTGCATGATTTCATTACATATGCGGCATCCGTTGATTTATTTACTATGATTGTGTCAAAGCCTGACTTTGTTTCCC
TTCTTCTGCTTGCGGTTTCCGTTTTTACGCTTTTTGCGGCTTTGGAAAAGGGAGGTTTTTTAAAACTCAGGAAATCGGCT
CTTTTTTTCTGCGCGGTTTTGGCTTATTTAATATGCCGTCCGTATTTCAGTCCATGGGGAGAAGCGGATATGCTTGATAT
CGGGCAGGGAGACTCACTGTTTATAAGCGCGCCGCACCGCAAAGGGACCGTAATGGTTGATACAGGGGGAGTGATTGCTT
ATCCCGGAGAGTCATGGAAAGAAAAACGCCACCCATATTCTATCGGCGAGAAGGTTTTGATTCCATTTTTAAACGGAAAA
GGGGTGAAAAAGCTGGATGCGCTGATTTTAACCCATGCGGATCAAGATCACATCGGGGAAGCCGGAGTGTTAATCAAAAA
CCATAGAGTCAAACGGTTAATTGTCCCCGTAGGATTCGTAAAAGAACCGAAAGATCAGAACATATTAAATATGGCGAAAG
AAAACAACATTCCCGTTGCCGAAGCAAAGCGGGGCGACACCATTACAGCCGGGGATCTTCAGTTTCAGGTGCTGTCTCCG
GAGTCGTCTGACGGAAGGAGTAAAAATGATTCGTCACTGGTGCTTTGGACGGTTTTTGGCGGAGTGAGCTGGCTTTTGAC
GGGAGATTTAGAATCGGACGGCGAAACGGAAGTGCTGAAAACGTATCCGAATCTGAAGGCTGATATATTGAAGGCGGGTC
ATCACGGCAGCAAAAGCTCAACGAGTGAAGCCTTTTTGAAGCAGCTTCAGCCGGAAGCAGCGCTGATTTCAGCAGGAAAA
GAGAATCGATACCATCATCCGCATGAAGAAGTGCTGGATCGTTTGAAGGCGTACTCTGTCAATGTGCTTCGCACCGATGT
CAGCGGAACGATTCAATACAGATTTAAAAAAGGCGCCGGAACGTTTTCCGTCTTCCCTCCATATGATATAGAAGAAACCA
GGGCGCAAGAAGTAAAAAAGACTGCCGATTGA

Domains

Predicted by InterproScan.

Domain of unknown function (DUF4131) (DUF4131)

(25-171)

Competence protein (Competence)

(208-469)

Metallo-beta-lactamase superfamily (Lactamase_B)

(508-719)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEC Bacillus subtilis subsp. subtilis str. 168

57.254

98.595

0.564