Detailed information NTG0944148

Bioinformatically predicted

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: V7A40_RS03950
• Genome accession: NZ_CP146750
• Coordinates: 796543..797799 (-)
• Length: 418 a.a.
• NCBI ID: WP_341785241.1
• Uniprot ID: -
• Organism: Lactococcus garvieae strain L2
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 791543..802799

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
V7A40_RS03915 (V7A40_03915)	rpmA	791664..791948 (+)	285	WP_004257956.1	50S ribosomal protein L27	-
V7A40_RS03920 (V7A40_03920)	-	792298..793404 (+)	1107	WP_341784750.1	alanine dehydrogenase	-
V7A40_RS03925 (V7A40_03925)	-	793522..794481 (+)	960	WP_004257953.1	PhoH family protein	-
V7A40_RS03930 (V7A40_03930)	-	794483..794956 (+)	474	WP_014024618.1	NUDIX hydrolase	-
V7A40_RS03935 (V7A40_03935)	ybeY	794966..795454 (+)	489	WP_014024619.1	rRNA maturation RNase YbeY	-
V7A40_RS03940 (V7A40_03940)	-	795438..795899 (+)	462	WP_014024620.1	diacylglycerol kinase family protein	-
V7A40_RS03945 (V7A40_03945)	-	795902..796546 (-)	645	WP_014024621.1	ComF family protein	-
V7A40_RS03950 (V7A40_03950)	comFA	796543..797799 (-)	1257	WP_341785241.1	DEAD/DEAH box helicase	Machinery gene
V7A40_RS03955 (V7A40_03955)	-	797854..798477 (+)	624	WP_004257938.1	YigZ family protein	-
V7A40_RS03960 (V7A40_03960)	-	798491..799513 (+)	1023	WP_014024623.1	DUF475 domain-containing protein	-
V7A40_RS03965 (V7A40_03965)	-	799545..800567 (-)	1023	WP_341785242.1	alpha/beta hydrolase	-
V7A40_RS03970 (V7A40_03970)	-	800886..801665 (+)	780	WP_014024625.1	formate/nitrite transporter family protein	-
V7A40_RS03975 (V7A40_03975)	glyQ	801679..802647 (+)	969	WP_004257927.1	glycine--tRNA ligase subunit alpha	-

Sequence

Protein

• Length: 418 a.a.
• Molecular weight: 48025.86 Da
• Isoelectric Point: 9.8402

>NTDB_id=944148 V7A40_RS03950 WP_341785241.1 796543..797799(-) (comFA) [Lactococcus garvieae strain L2]
MDELYGRLLLQKELTKLPDKATLFDGMQDVSKTVMMCNRCGKKIKKKEVLLPVGAYYCPHCIQMGRVRSDEKLYHLPQED
FSAASFLNWQGKLTASQQHVSDSLVKLQQQQKTVLVQAVTGAGKTEMIYQSIDNTLKKGKAVGLTSPRIDVCLELYHRLK
RDFSCPISLLHGKSEKYSRSPLVIATTHQLMRFRHAFDLLILDEVDAFPFPDNEMLYFELAQARKPSSILIYLTATTTDN
LEKQVKLGQIEKLQLPRRFHGFPLVLPQFFWQSKFYKMIKKQRESGFPLLIFVPEIRQEEKLCQDLQSNFPHEEIAFVAS
TSLERLEAVERFRQGNISILVSTTILERGVTFPKVDVFVFQSHHHNFTRSSLIQIAGRVGRSTERPEGKVFFFHLGKTTA
MLEAYKNIRNMNKAGGFL

Nucleotide

• Length: 1257 bp

>NTDB_id=944148 V7A40_RS03950 WP_341785241.1 796543..797799(-) (comFA) [Lactococcus garvieae strain L2]
ATGGATGAATTATATGGTAGACTCCTTTTACAAAAAGAATTGACGAAACTTCCAGATAAAGCAACACTTTTTGATGGGAT
GCAAGATGTTTCCAAAACAGTGATGATGTGCAACCGCTGTGGCAAAAAGATCAAGAAAAAAGAGGTCCTTCTCCCTGTTG
GTGCTTATTATTGTCCCCATTGTATTCAAATGGGACGTGTTCGCTCGGATGAAAAACTTTACCACCTTCCCCAGGAGGAT
TTTTCAGCAGCTTCTTTTCTCAACTGGCAGGGAAAACTCACAGCATCTCAGCAACATGTATCCGATAGCCTAGTCAAATT
GCAACAGCAACAAAAAACAGTACTGGTACAGGCGGTTACTGGTGCAGGAAAAACCGAAATGATTTATCAGAGCATTGATA
ACACCCTTAAAAAAGGAAAAGCTGTAGGTTTAACTAGCCCGCGTATTGATGTCTGTCTAGAGCTCTATCATCGCTTAAAG
AGAGATTTTTCTTGTCCCATCTCGCTTTTGCACGGAAAGAGTGAAAAATACAGCCGCTCGCCACTCGTAATCGCAACGAC
ACATCAGCTTATGCGTTTTCGCCATGCCTTTGACTTACTCATTCTTGATGAGGTGGATGCCTTTCCCTTTCCAGACAATG
AAATGCTTTATTTTGAGCTGGCTCAAGCCCGTAAGCCTTCTTCAATCTTGATTTATCTTACTGCAACCACCACCGATAAT
TTGGAAAAACAAGTCAAGTTAGGACAAATCGAGAAGCTACAGCTTCCACGACGTTTTCATGGTTTTCCTCTGGTACTCCC
GCAATTTTTTTGGCAAAGCAAATTTTACAAAATGATCAAAAAACAACGCGAGTCTGGTTTTCCCTTACTTATCTTTGTTC
CTGAAATAAGACAGGAAGAAAAACTCTGCCAAGATTTACAGAGTAATTTTCCTCATGAAGAAATTGCTTTTGTCGCGTCA
ACAAGTCTTGAACGCTTAGAGGCTGTGGAACGCTTTCGTCAGGGCAATATTTCTATCCTTGTCTCAACGACCATTTTAGA
ACGTGGTGTAACTTTCCCTAAGGTTGACGTTTTTGTGTTTCAAAGTCACCACCATAATTTCACAAGATCAAGCCTCATCC
AAATTGCCGGACGAGTAGGTCGAAGTACCGAAAGACCTGAGGGTAAGGTATTTTTCTTTCATTTAGGAAAAACTACAGCG
ATGTTGGAAGCCTATAAAAATATCAGAAATATGAATAAAGCAGGAGGGTTCCTATGA

Domains

Predicted by InterproScan.

• Type III restriction enzyme, res subunit (ResIII) (92-237)

• Helicase conserved C-terminal domain (Helicase_C) (274-382)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Lactococcus lactis subsp. cremoris KW2	57.143	93.78	0.536
comFA/cflA	Streptococcus mitis SK321	52.113	100	0.531
comFA/cflA	Streptococcus pneumoniae TIGR4	52.113	100	0.531
comFA/cflA	Streptococcus pneumoniae Rx1	52.113	100	0.531
comFA/cflA	Streptococcus pneumoniae D39	52.113	100	0.531
comFA/cflA	Streptococcus pneumoniae R6	52.113	100	0.531
comFA/cflA	Streptococcus mitis NCTC 12261	51.174	100	0.522
comFA	Bacillus subtilis subsp. subtilis str. 168	38.366	96.651	0.371
comFA	Latilactobacillus sakei subsp. sakei 23K	38.287	94.976	0.364