Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilR   Type   Regulator
Locus tag   PSGK_RS28725 Genome accession   NZ_CP146285
Coordinates   6341111..6342445 (-) Length   444 a.a.
NCBI ID   WP_021219360.1    Uniprot ID   A0AAU7Y6G6
Organism   Pseudomonas solani strain GK13     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 6316615..6341098 6341111..6342445 flank 13


Gene organization within MGE regions


Location: 6316615..6342445
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSGK_RS28595 (PSGK_28445) - 6316615..6317109 (+) 495 WP_031287650.1 TIGR00645 family protein -
  PSGK_RS28600 (PSGK_28450) - 6317240..6317545 (+) 306 WP_021219384.1 DUF6482 family protein -
  PSGK_RS28605 (PSGK_28455) - 6317631..6318248 (+) 618 WP_021219383.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  PSGK_RS28610 (PSGK_28460) - 6318388..6318636 (-) 249 WP_021219382.1 hypothetical protein -
  PSGK_RS28615 (PSGK_28465) - 6318865..6319833 (-) 969 WP_021219381.1 polyprenyl synthetase family protein -
  PSGK_RS28620 (PSGK_28470) rplU 6320078..6320389 (+) 312 WP_021219380.1 50S ribosomal protein L21 -
  PSGK_RS28625 (PSGK_28475) rpmA 6320415..6320672 (+) 258 WP_031287649.1 50S ribosomal protein L27 -
  PSGK_RS28630 (PSGK_28480) cgtA 6320814..6322034 (+) 1221 WP_316925391.1 Obg family GTPase CgtA -
  PSGK_RS28635 (PSGK_28485) proB 6322115..6323233 (+) 1119 WP_263400623.1 glutamate 5-kinase -
  PSGK_RS28640 (PSGK_28490) - 6323246..6323710 (+) 465 WP_374979893.1 CreA family protein -
  PSGK_RS28645 (PSGK_28495) rpsT 6323820..6324095 (-) 276 WP_031287647.1 30S ribosomal protein S20 -
  PSGK_RS28650 (PSGK_28500) murJ 6324357..6325892 (+) 1536 WP_374979894.1 murein biosynthesis integral membrane protein MurJ -
  PSGK_RS28655 (PSGK_28505) ribF 6326001..6326939 (+) 939 WP_265167680.1 bifunctional riboflavin kinase/FAD synthetase -
  PSGK_RS28660 (PSGK_28510) ileS 6326955..6329786 (+) 2832 WP_271660051.1 isoleucine--tRNA ligase -
  PSGK_RS28665 (PSGK_28515) lspA 6329779..6330285 (+) 507 WP_263400619.1 signal peptidase II -
  PSGK_RS28670 (PSGK_28520) - 6330278..6330730 (+) 453 WP_043243724.1 peptidylprolyl isomerase -
  PSGK_RS28675 (PSGK_28525) ispH 6330780..6331724 (+) 945 WP_374979895.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  PSGK_RS28680 (PSGK_28530) - 6331779..6332216 (-) 438 WP_316925386.1 type IV pilin protein -
  PSGK_RS28685 (PSGK_28535) - 6332216..6336520 (-) 4305 WP_374979896.1 pilus assembly protein PilY -
  PSGK_RS28690 (PSGK_28540) - 6336536..6336880 (-) 345 WP_374979897.1 hypothetical protein -
  PSGK_RS28695 (PSGK_28545) - 6336896..6337438 (-) 543 WP_184488602.1 PilX N-terminal domain-containing pilus assembly protein -
  PSGK_RS28700 (PSGK_28550) - 6337448..6338257 (-) 810 WP_374979898.1 PilW family protein -
  PSGK_RS28705 (PSGK_28555) pilV 6338254..6338829 (-) 576 WP_184488603.1 type IV pilus modification protein PilV -
  PSGK_RS28710 (PSGK_28560) - 6338816..6339334 (-) 519 WP_374979899.1 GspH/FimT family pseudopilin -
  PSGK_RS28715 (PSGK_28565) - 6339449..6339958 (-) 510 WP_374979900.1 GspH/FimT family protein -
  PSGK_RS28720 (PSGK_28570) thiO 6340001..6341098 (+) 1098 WP_374979901.1 glycine oxidase ThiO -
  PSGK_RS28725 (PSGK_28575) pilR 6341111..6342445 (-) 1335 WP_021219360.1 sigma-54 dependent transcriptional regulator Regulator

Sequence

Protein


Download         Length: 444 a.a.        Molecular weight: 49586.77 Da        Isoelectric Point: 5.4271

>NTDB_id=942507 PSGK_RS28725 WP_021219360.1 6341111..6342445(-) (pilR) [Pseudomonas solani strain GK13]
MTRQKALIVDDEPDIRELLEITLGRMKLDTRSARNVKEARELLAAEPFDLCLTDMRLPDGTGLDLVQHIQQRHPQVPVAM
ITAYGSLDTAVNALKAGAFDFLTKPVDLGRLRELVAAALRLRTSSNGDTPMDNRLLGDSPPMKSLRNQIQKLARSQAPVY
ISGESGSGKELVARMIHEQGPRHEDPFVPVNCGAIPSELMESEFFGHKKGSFTGAIEDKIGLFQAAHGGTLFLDEVADLP
LPMQVKLLRAIQEKAVRTIGGQQEVVVDVRILSATHKDLAAEVAAGRFRQDLYYRLNVIELSVPPLRERREDIPQLADVM
LKRLAADSSAAASITRDAMEKLKSYRFPGNVRELENMLERAYTLCENDQIQPEDLRLTDAPAASENGEADLAQIDNLEDY
LEEVERKLIMQALEETRWNRTAAAQRLGLTFRSMRYRLKKLGID

Nucleotide


Download         Length: 1335 bp        

>NTDB_id=942507 PSGK_RS28725 WP_021219360.1 6341111..6342445(-) (pilR) [Pseudomonas solani strain GK13]
ATGACCAGACAGAAAGCACTCATCGTCGATGACGAGCCCGATATCCGCGAACTGCTGGAGATCACCCTCGGCCGGATGAA
GCTGGATACCCGCAGCGCCCGCAACGTGAAAGAGGCCCGCGAGCTCCTGGCCGCCGAACCCTTCGACCTGTGCCTCACCG
ACATGCGCCTGCCCGACGGCACCGGCCTCGACCTCGTTCAGCACATCCAGCAACGCCACCCCCAAGTGCCCGTTGCCATG
ATTACCGCCTACGGCAGCCTGGACACGGCCGTCAACGCCCTGAAAGCCGGCGCCTTCGACTTTCTTACCAAACCGGTGGA
CCTCGGCCGGCTTCGCGAGCTGGTGGCTGCGGCCTTGCGCCTGCGCACCAGCAGCAACGGCGACACGCCGATGGACAACC
GCCTGCTCGGCGACTCCCCGCCGATGAAGTCTCTGCGTAACCAGATCCAGAAGCTCGCCCGCAGCCAGGCCCCCGTGTAC
ATCAGCGGCGAATCCGGCAGCGGCAAGGAGCTGGTGGCCCGCATGATCCACGAGCAGGGCCCGCGTCATGAAGACCCCTT
CGTACCGGTCAACTGCGGCGCCATCCCGTCGGAGCTGATGGAAAGCGAGTTCTTCGGCCACAAGAAAGGCAGCTTCACCG
GCGCCATCGAAGACAAGATCGGCCTGTTCCAGGCTGCCCACGGTGGCACCCTGTTCCTCGATGAAGTGGCCGACCTGCCC
CTGCCCATGCAGGTGAAACTGCTGCGCGCCATCCAGGAAAAAGCCGTCCGCACCATCGGCGGTCAGCAGGAAGTGGTGGT
GGACGTGCGCATCCTCAGCGCCACCCACAAGGACCTGGCCGCAGAAGTGGCTGCAGGACGCTTCCGCCAGGACCTCTATT
ACCGCCTGAACGTCATCGAACTCAGCGTCCCGCCGCTGCGCGAGCGCCGCGAGGACATCCCGCAACTGGCCGACGTCATG
CTCAAGCGCCTGGCAGCGGACAGCAGCGCCGCAGCCAGCATCACCCGCGACGCGATGGAGAAGCTCAAGAGCTATCGCTT
CCCCGGCAACGTGCGCGAGCTGGAGAACATGCTGGAGCGGGCCTACACACTGTGTGAGAACGACCAGATCCAGCCTGAAG
ACCTGCGCCTGACGGATGCACCGGCGGCTTCCGAAAACGGCGAAGCCGACCTCGCGCAGATCGACAATCTCGAGGACTAC
CTGGAAGAGGTGGAGCGCAAGCTGATCATGCAGGCGCTCGAGGAAACCCGCTGGAACCGCACCGCCGCAGCCCAACGCCT
GGGCCTGACCTTCCGCTCGATGCGCTACCGCCTGAAGAAACTGGGGATCGACTGA

Domains

Predicted by InterproScan.

Sigma-54 interaction domain (Sigma54_activat)

(136-301)

Response regulator receiver domain (Response_reg)

(7-115)

Bacterial regulatory protein, Fis family (HTH_8)

(401-441)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilR Pseudomonas aeruginosa PAK

86.742

100

0.869
  pilR Acinetobacter baumannii strain A118

49.783

100

0.516
  luxO Vibrio cholerae strain A1552

37.104

99.55

0.369