Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   V8H04_RS08660 Genome accession   NZ_CP146210
Coordinates   1742952..1743449 (-) Length   165 a.a.
NCBI ID   WP_001803317.1    Uniprot ID   A0A2I7Y542
Organism   Staphylococcus aureus strain KWT 2020-26218     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1737952..1748449
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V8H04_RS08635 (V8H04_08630) gcvPB 1738114..1739586 (-) 1473 WP_000202189.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  V8H04_RS08640 (V8H04_08635) gcvPA 1739579..1740925 (-) 1347 WP_000019687.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  V8H04_RS08645 (V8H04_08640) gcvT 1740945..1742036 (-) 1092 WP_000093351.1 glycine cleavage system aminomethyltransferase GcvT -
  V8H04_RS08650 (V8H04_08645) - 1742195..1742719 (-) 525 WP_001015117.1 shikimate kinase -
  V8H04_RS08655 (V8H04_08650) - 1742709..1742855 (-) 147 WP_001789879.1 hypothetical protein -
  V8H04_RS08660 (V8H04_08655) comGF 1742952..1743449 (-) 498 WP_001803317.1 competence type IV pilus minor pilin ComGF Machinery gene
  V8H04_RS08665 (V8H04_08660) comGE 1743367..1743666 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  V8H04_RS08670 (V8H04_08665) comGD 1743653..1744099 (-) 447 WP_001790850.1 competence type IV pilus minor pilin ComGD Machinery gene
  V8H04_RS08675 (V8H04_08670) comGC 1744077..1744388 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  V8H04_RS08680 (V8H04_08675) comGB 1744402..1745472 (-) 1071 WP_000775706.1 competence type IV pilus assembly protein ComGB Machinery gene
  V8H04_RS08685 (V8H04_08680) comGA 1745444..1746418 (-) 975 WP_000697228.1 competence type IV pilus ATPase ComGA Machinery gene
  V8H04_RS08690 (V8H04_08685) - 1746470..1747093 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  V8H04_RS08695 (V8H04_08690) - 1747090..1747419 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  V8H04_RS08700 (V8H04_08695) - 1747419..1748405 (-) 987 WP_000161314.1 ROK family glucokinase -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19212.92 Da        Isoelectric Point: 10.2192

>NTDB_id=942002 V8H04_RS08660 WP_001803317.1 1742952..1743449(-) (comGF) [Staphylococcus aureus strain KWT 2020-26218]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMFISITLLIVPDLIRLSKTFLIESRDLTTVDFEFFSRDI
LDDFKGVDRNDIEIRQHRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNVQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=942002 V8H04_RS08660 WP_001803317.1 1742952..1743449(-) (comGF) [Staphylococcus aureus strain KWT 2020-26218]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTCATTGAAATGTTAGTAGCGATGATGTTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGTAAAACTTTTCTAATTGAAAGTAGGGATTTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGATGATTTTAAAGGAGTAGATAGAAACGATATTGAAATTAGGCAACACCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATTAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(67-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2I7Y542

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus MW2

99.394

100

0.994
  comGF Staphylococcus aureus N315

95.758

100

0.958