Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   V8H04_RS08685 Genome accession   NZ_CP146210
Coordinates   1745444..1746418 (-) Length   324 a.a.
NCBI ID   WP_000697228.1    Uniprot ID   A0A0D3Q868
Organism   Staphylococcus aureus strain KWT 2020-26218     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1740444..1751418
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V8H04_RS08645 (V8H04_08640) gcvT 1740945..1742036 (-) 1092 WP_000093351.1 glycine cleavage system aminomethyltransferase GcvT -
  V8H04_RS08650 (V8H04_08645) - 1742195..1742719 (-) 525 WP_001015117.1 shikimate kinase -
  V8H04_RS08655 (V8H04_08650) - 1742709..1742855 (-) 147 WP_001789879.1 hypothetical protein -
  V8H04_RS08660 (V8H04_08655) comGF 1742952..1743449 (-) 498 WP_001803317.1 competence type IV pilus minor pilin ComGF Machinery gene
  V8H04_RS08665 (V8H04_08660) comGE 1743367..1743666 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  V8H04_RS08670 (V8H04_08665) comGD 1743653..1744099 (-) 447 WP_001790850.1 competence type IV pilus minor pilin ComGD Machinery gene
  V8H04_RS08675 (V8H04_08670) comGC 1744077..1744388 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  V8H04_RS08680 (V8H04_08675) comGB 1744402..1745472 (-) 1071 WP_000775706.1 competence type IV pilus assembly protein ComGB Machinery gene
  V8H04_RS08685 (V8H04_08680) comGA 1745444..1746418 (-) 975 WP_000697228.1 competence type IV pilus ATPase ComGA Machinery gene
  V8H04_RS08690 (V8H04_08685) - 1746470..1747093 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  V8H04_RS08695 (V8H04_08690) - 1747090..1747419 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  V8H04_RS08700 (V8H04_08695) - 1747419..1748405 (-) 987 WP_000161314.1 ROK family glucokinase -
  V8H04_RS08705 (V8H04_08700) - 1748402..1748605 (-) 204 WP_000087561.1 YqgQ family protein -
  V8H04_RS08710 (V8H04_08705) - 1748586..1750049 (-) 1464 WP_001017867.1 rhomboid family intramembrane serine protease -
  V8H04_RS08715 (V8H04_08710) - 1750061..1750600 (-) 540 WP_000164545.1 5-formyltetrahydrofolate cyclo-ligase -
  V8H04_RS08720 (V8H04_08715) rpmG 1750809..1750958 (-) 150 WP_001265709.1 50S ribosomal protein L33 -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36865.01 Da        Isoelectric Point: 9.0399

>NTDB_id=942007 V8H04_RS08685 WP_000697228.1 1745444..1746418(-) (comGA) [Staphylococcus aureus strain KWT 2020-26218]
MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYLF
NKIYFLRISTLPLSLGQESCVIRIVPQFFQPQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHATDCKGAI
LRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTM
HKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=942007 V8H04_RS08685 WP_000697228.1 1745444..1746418(-) (comGA) [Staphylococcus aureus strain KWT 2020-26218]
TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTACATTTTATTCCAGTTAAAAA
TGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAGCAGTATGAACAAATTGGGAATAGCATTTATCAAAAGTTAT
TAGTTTATATGAAGTTTCAAGCTGGGCTTGATGTTTCTACACAGCAAGTCGCACAGAGCGGTCGATATAGTTACCTTTTC
AATAAAATATATTTTTTGAGAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGTGTTATCAGAATTGTACCTCA
ATTTTTTCAACCACAGAAATCAACTTATAAATTCAATGATTTTAAACACCTCATGAATAAGAAACAAGGATTACTATTGT
TTAGTGGACCAACTGGTTCAGGAAAGAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCTTGAATTTAAAT
GTAATTTCTATAGAGGATCCTGTAGAGATGCAAATTCCTGGTATCGTCCAAATTAATGTGAATGATAAAGCTGGCATAAA
CTATGTAAATTCGTTTAAAGCTATTTTAAGATGTGATCCTGATGTTATTTTAATAGGTGAAATCAGAGATAAAGATGTTG
CCAAGTGTGTTATACAGGCTAGTTTAAGTGGTCACCTTGTTCTGACTACATTGCATGCAACTGATTGTAAAGGTGCTATT
TTAAGGCTATTAGAAATGGGCATTTCTGTACAAGAATTGATACAGGCAACTAACTTAATTATAAACCAACGACTTGTAAC
TACTATTAAGCAACAGCGACAATTAGTATGTGAAATTCTATCTCAGCAACAACTCCGATATTTCTTTTCCCATAATCATT
CATTACCATCATCATTTAAGAACTTAGAAGATAAACTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAACTACAATG
CATAAATACATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D3Q868

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

99.074

100

0.991
  comGA Staphylococcus aureus N315

99.074

100

0.991