Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   V6L81_RS01505 Genome accession   NZ_CP146077
Coordinates   335770..337101 (-) Length   443 a.a.
NCBI ID   WP_095000850.1    Uniprot ID   -
Organism   Pseudomonas bubulae strain ML3-2023-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 330770..342101
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V6L81_RS01490 (V6L81_01490) - 332434..334005 (-) 1572 WP_095017725.1 AAA family ATPase -
  V6L81_RS01495 (V6L81_01495) aroB 334016..335116 (-) 1101 WP_095000851.1 3-dehydroquinate synthase -
  V6L81_RS01500 (V6L81_01500) aroK 335247..335765 (-) 519 WP_029611693.1 shikimate kinase AroK -
  V6L81_RS01505 (V6L81_01505) pilQ 335770..337101 (-) 1332 WP_095000850.1 type IV pilus secretin PilQ Machinery gene
  V6L81_RS01510 (V6L81_01510) - 337114..337608 (-) 495 WP_232527659.1 pilus assembly protein PilP -
  V6L81_RS01515 (V6L81_01515) - 337646..338260 (-) 615 WP_257010077.1 type 4a pilus biogenesis protein PilO -
  V6L81_RS01520 (V6L81_01520) - 338257..338823 (-) 567 WP_095000848.1 PilN domain-containing protein -
  V6L81_RS01525 (V6L81_01525) pilM 338823..339785 (-) 963 WP_095026343.1 type IV pilus biogenesis protein PilM -

Sequence

Protein


Download         Length: 443 a.a.        Molecular weight: 47812.11 Da        Isoelectric Point: 5.3850

>NTDB_id=941589 V6L81_RS01505 WP_095000850.1 335770..337101(-) (pilQ) [Pseudomonas bubulae strain ML3-2023-1]
MKRISPVCVLALWSALNSQSMLAAGQAAPSSVKRSQYVGEPMSLSVQNLDVRTVLQSLADFTGFNLMVSDTVKGSITLSL
QDVPWDQALELVLESKGLGKRLEGNVLLIAPLEEIAARERPEPAPLRRELVQVNYARAADIVMLFQSVTGGQPGIEGQGT
ITVDERTNTIIAYESSERLEELRRIVAQLDVPVRQVMIEAWIVEAGVDFEKSLGVRWGGALNNGGLWKAGGITQPAASGA
APGNVSAPFVDLGVSRNTSGLGIAFITNNVLLDLELTAMEKTGNGEIISQPKVVTADKETAKILKGTQIPYQQASKTGAT
SVSFKEASLSLEVTPQITPDNSVIMAVVVTKDEPDYMSRLQDVPPIKKNEVRAKVLVKDGETIVIGGVFSKTQSKVVDKV
PFLGDLPYVGRLFRRDVVLEKKSELLVFLTPRIMNNQAIAVSH

Nucleotide


Download         Length: 1332 bp        

>NTDB_id=941589 V6L81_RS01505 WP_095000850.1 335770..337101(-) (pilQ) [Pseudomonas bubulae strain ML3-2023-1]
ATGAAAAGGATTTCACCGGTCTGCGTACTGGCGCTATGGAGTGCGCTGAATTCGCAGTCAATGCTGGCGGCTGGGCAGGC
CGCGCCATCATCCGTCAAGCGCTCGCAGTATGTGGGCGAGCCCATGTCCCTGAGCGTGCAGAATCTTGATGTACGCACGG
TACTGCAATCGCTGGCGGATTTTACCGGGTTCAACCTGATGGTCAGCGACACCGTAAAGGGCAGCATAACCCTCAGTTTG
CAGGATGTGCCTTGGGATCAGGCGCTGGAGCTGGTGCTTGAGAGCAAGGGGTTGGGCAAGCGTCTTGAAGGTAATGTGTT
GTTGATCGCGCCCCTGGAAGAGATCGCAGCCCGCGAGCGCCCTGAGCCTGCGCCTTTGCGTCGGGAGTTGGTGCAAGTCA
ATTACGCCAGGGCGGCAGATATCGTCATGCTGTTCCAGTCAGTGACCGGCGGGCAGCCCGGCATCGAGGGGCAGGGCACG
ATCACGGTGGATGAACGCACCAATACCATCATTGCTTATGAATCGAGTGAACGGCTGGAGGAGCTGCGGCGCATCGTGGC
TCAACTGGACGTGCCAGTGCGTCAGGTGATGATCGAAGCCTGGATTGTCGAGGCAGGCGTGGACTTCGAAAAAAGCCTGG
GTGTGCGTTGGGGGGGAGCGCTAAACAATGGTGGCCTCTGGAAAGCCGGTGGTATTACCCAGCCTGCGGCGTCCGGGGCT
GCCCCTGGCAACGTTAGTGCGCCCTTTGTTGATTTGGGTGTTTCGCGAAATACCTCGGGCTTGGGCATCGCCTTCATCAC
CAACAATGTGTTGCTGGACCTGGAACTGACGGCCATGGAAAAAACCGGTAATGGGGAAATCATCTCGCAACCCAAAGTGG
TTACTGCGGATAAGGAAACCGCCAAAATTCTTAAAGGCACCCAAATTCCTTACCAGCAGGCCAGCAAAACCGGGGCTACA
TCGGTTTCGTTCAAGGAAGCCTCATTGTCACTGGAGGTGACGCCGCAAATCACTCCGGACAACAGCGTCATCATGGCCGT
CGTAGTGACCAAGGATGAGCCCGATTACATGAGCAGGCTGCAGGATGTACCGCCCATCAAGAAGAACGAGGTCAGGGCCA
AGGTGCTGGTCAAGGACGGTGAAACCATCGTGATTGGGGGAGTGTTCTCAAAAACTCAAAGCAAAGTGGTAGATAAAGTG
CCATTTTTAGGCGATCTGCCGTATGTTGGCCGACTTTTCCGTAGAGACGTTGTCCTGGAAAAAAAATCCGAACTGCTGGT
GTTCCTGACTCCGCGTATCATGAACAACCAGGCGATTGCTGTGAGTCATTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(69-111)

Bacterial type II/III secretion system short domain (Secretin_N)

(130-195)

Bacterial type II and III secretion system protein (Secretin)

(278-434)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

65.826

98.42

0.648
  comQ Acinetobacter baylyi ADP1

42.325

100

0.436
  pilQ Acinetobacter baumannii strain A118

39.832

100

0.429
  pilQ Acinetobacter baumannii D1279779

39.832

100

0.429
  pilQ Legionella pneumophila strain ERS1305867

42.009

98.871

0.415
  pilQ Legionella pneumophila strain Lp02

41.686

99.097

0.413
  pilQ Vibrio campbellii strain DS40M4

40

99.323

0.397
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

39.953

96.614

0.386
  pilQ Vibrio cholerae strain A1552

39.953

96.614

0.386