Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   V6Z34_RS13200 Genome accession   NZ_CP145817
Coordinates   2695307..2695921 (-) Length   204 a.a.
NCBI ID   WP_015387983.1    Uniprot ID   A0A6A8LHQ3
Organism   Bacillus velezensis strain WH-7     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2690307..2700921
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V6Z34_RS13170 rpsT 2690607..2690873 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  V6Z34_RS13175 holA 2690890..2691931 (-) 1042 Protein_2550 DNA polymerase III subunit delta -
  V6Z34_RS13180 - 2691971..2692125 (-) 155 Protein_2551 hypothetical protein -
  V6Z34_RS13185 - 2692166..2692300 (+) 135 WP_003152870.1 YqzM family protein -
  V6Z34_RS13190 comEC 2692319..2694670 (-) 2352 WP_063174744.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  V6Z34_RS13195 - 2694671..2695240 (-) 570 WP_003152868.1 ComE operon protein 2 -
  V6Z34_RS13200 comEA 2695307..2695921 (-) 615 WP_015387983.1 helix-hairpin-helix domain-containing protein Machinery gene
  V6Z34_RS13205 comER 2695980..2696801 (+) 822 WP_014305449.1 late competence protein ComER -
  V6Z34_RS13210 - 2696870..2697607 (-) 738 WP_014305450.1 class I SAM-dependent methyltransferase -
  V6Z34_RS13215 rsfS 2697604..2697960 (-) 357 WP_003152864.1 ribosome silencing factor -
  V6Z34_RS13220 yqeK 2697957..2698538 (-) 582 WP_063174743.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  V6Z34_RS13225 - 2698528..2699097 (-) 570 WP_003152860.1 nicotinate-nucleotide adenylyltransferase -
  V6Z34_RS13230 yhbY 2699108..2699398 (-) 291 WP_003152858.1 ribosome assembly RNA-binding protein YhbY -
  V6Z34_RS13235 aroE 2699392..2700234 (-) 843 WP_025852995.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22255.41 Da        Isoelectric Point: 5.5211

>NTDB_id=940112 V6Z34_RS13200 WP_015387983.1 2695307..2695921(-) (comEA) [Bacillus velezensis strain WH-7]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=940112 V6Z34_RS13200 WP_015387983.1 2695307..2695921(-) (comEA) [Bacillus velezensis strain WH-7]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGTGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGTTGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCTATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6A8LHQ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

58.854

94.118

0.554
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

35.545

100

0.368
  comEA/celA/cilE Streptococcus mitis SK321

35.071

100

0.363