Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   V6Z34_RS12775 Genome accession   NZ_CP145817
Coordinates   2618803..2619873 (-) Length   356 a.a.
NCBI ID   WP_003153083.1    Uniprot ID   -
Organism   Bacillus velezensis strain WH-7     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2613803..2624873
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V6Z34_RS12725 sipW 2613864..2614447 (-) 584 Protein_2461 signal peptidase I SipW -
  V6Z34_RS12730 tapA 2614419..2615090 (-) 672 WP_063174755.1 amyloid fiber anchoring/assembly protein TapA -
  V6Z34_RS12735 - 2615349..2615678 (+) 330 WP_003153097.1 DUF3889 domain-containing protein -
  V6Z34_RS12740 - 2615718..2615897 (-) 180 WP_003153093.1 YqzE family protein -
  V6Z34_RS12745 comGG 2615954..2616331 (-) 378 WP_014305410.1 competence type IV pilus minor pilin ComGG Machinery gene
  V6Z34_RS12750 comGF 2616332..2616832 (-) 501 WP_232789965.1 competence type IV pilus minor pilin ComGF -
  V6Z34_RS12755 comGE 2616741..2617055 (-) 315 WP_041481885.1 competence type IV pilus minor pilin ComGE Machinery gene
  V6Z34_RS12760 comGD 2617039..2617476 (-) 438 WP_095318402.1 competence type IV pilus minor pilin ComGD Machinery gene
  V6Z34_RS12765 comGC 2617466..2617774 (-) 309 WP_003153087.1 competence type IV pilus major pilin ComGC Machinery gene
  V6Z34_RS12770 comGB 2617779..2618816 (-) 1038 WP_024085602.1 competence type IV pilus assembly protein ComGB Machinery gene
  V6Z34_RS12775 comGA 2618803..2619873 (-) 1071 WP_003153083.1 competence type IV pilus ATPase ComGA Machinery gene
  V6Z34_RS12780 - 2620065..2621015 (-) 951 WP_003153082.1 magnesium transporter CorA family protein -
  V6Z34_RS12785 - 2621161..2622460 (+) 1300 Protein_2473 hemolysin family protein -
  V6Z34_RS12790 - 2622498..2623328 (-) 831 WP_003153080.1 STAS domain-containing protein -
  V6Z34_RS12800 - 2623534..2623914 (-) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  V6Z34_RS12805 - 2624151..2624396 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40529.85 Da        Isoelectric Point: 9.4729

>NTDB_id=940108 V6Z34_RS12775 WP_003153083.1 2618803..2619873(-) (comGA) [Bacillus velezensis strain WH-7]
MKNIETYSRNIINEAYTARASDIHIVPREKDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=940108 V6Z34_RS12775 WP_003153083.1 2618803..2619873(-) (comGA) [Bacillus velezensis strain WH-7]
GTGAAAAATATTGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAAGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTTATTTCTCATTTCAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGTTCATTGTCT
CTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAACTGTCCTTATTTCGGGGGGCGGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCACACGGCCTGCTTCTGTTTACCGGCCCGACCGGCTCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAACGGCATTTCAACCGGCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGTTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGAGATGCCGAAACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTTGGGGTGAACATGACAGAAATTGAACAAACCGTCATTGC
CATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACCCGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACAACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.022

100

0.82
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.892

98.596

0.374