Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   ACEXP4_RS29920 Genome accession   NZ_CP169522
Coordinates   6217487..6218110 (+) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain CIMT2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6212487..6223110
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEXP4_RS29905 (ACEXP4_29905) - 6213174..6215645 (-) 2472 WP_375080046.1 penicillin-binding protein 1A -
  ACEXP4_RS29910 (ACEXP4_29910) pilM 6215830..6216894 (+) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  ACEXP4_RS29915 (ACEXP4_29915) pilN 6216894..6217490 (+) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  ACEXP4_RS29920 (ACEXP4_29920) pilO 6217487..6218110 (+) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACEXP4_RS29925 (ACEXP4_29925) comL/pilP 6218107..6218631 (+) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  ACEXP4_RS29930 (ACEXP4_29930) pilQ 6218685..6220829 (+) 2145 WP_003103267.1 type 4a pilus secretin PilQ Machinery gene
  ACEXP4_RS29935 (ACEXP4_29935) aroK 6220841..6221359 (+) 519 WP_003095833.1 shikimate kinase AroK -
  ACEXP4_RS29940 (ACEXP4_29940) aroB 6221409..6222515 (+) 1107 WP_019726985.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=939211 ACEXP4_RS29920 WP_003103268.1 6217487..6218110(+) (pilO) [Pseudomonas aeruginosa strain CIMT2]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=939211 ACEXP4_RS29920 WP_003103268.1 6217487..6218110(+) (pilO) [Pseudomonas aeruginosa strain CIMT2]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGTGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTTGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACTTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401