Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   V5G09_RS00495 Genome accession   NZ_CP145088
Coordinates   103612..105783 (-) Length   723 a.a.
NCBI ID   WP_003698035.1    Uniprot ID   Q5FAD2
Organism   Neisseria gonorrhoeae strain WHO_B_2024     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 98612..110783
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V5G09_RS00480 (V5G09_00485) - 99086..99928 (-) 843 WP_003687345.1 class II glutamine amidotransferase -
  V5G09_RS00485 (V5G09_00490) aroB 100671..101750 (-) 1080 WP_003690567.1 3-dehydroquinate synthase -
  V5G09_RS00490 (V5G09_00495) - 101830..102342 (-) 513 WP_003687349.1 shikimate kinase -
  V5G09_RS00495 (V5G09_00500) pilQ 103612..105783 (-) 2172 WP_003698035.1 type IV pilus secretin PilQ Machinery gene
  V5G09_RS00500 (V5G09_00505) pilP 105802..106347 (-) 546 WP_003692554.1 pilus assembly protein PilP Machinery gene
  V5G09_RS00505 (V5G09_00510) - 106365..107012 (-) 648 WP_003690570.1 type 4a pilus biogenesis protein PilO -
  V5G09_RS00510 (V5G09_00515) pilN 107013..107612 (-) 600 WP_003687354.1 PilN domain-containing protein Machinery gene
  V5G09_RS00515 (V5G09_00520) pilM 107615..108730 (-) 1116 WP_003687356.1 type IV pilus biogenesis protein PilM -

Sequence

Protein


Download         Length: 723 a.a.        Molecular weight: 77950.93 Da        Isoelectric Point: 10.0021

>NTDB_id=937116 V5G09_RS00495 WP_003698035.1 103612..105783(-) (pilQ) [Neisseria gonorrhoeae strain WHO_B_2024]
MNTKLTKIISGLFVATAAFQTASAGNITDIKVSSLPNKQKIVKVSFDKEIVNPTGFVTSSPARIALDFEQTGISMDQQVL
EYADPLLSKISAAQNSSRARLVLNLNKPGQYNTEVRGNKVWIFINESDDTVSAPARPAVKAAPAAPAKQQAAAPFTESVV
SVSAPFSPAKQQAAASAKQTNIDFRKDGKNAGIIELAALGFAGQPDISQQHDHIIVTLKNHTLPTALQRSLDVADFKTPV
QKVTLKRLNNDTQLIITTTGNWELVNKSAAPGYFTFQVLPKKQNLESGGVNNAPKTFTGRKISLDFQDVEIRTILQILAK
ESGMNIVASDSVSGKMTLSLKDVPWDQALDLVMQARNLDMRQQGNIVNIAPRDELLAKDKAFLQAEKDIADLGALYSQNF
QLKYKNVEEFRSILRLDNADTTGNRNTLVSGRGSVLIDPATNTLIVTDTRSVIEKFRKLIDELDVPAQQVMIEARIVEAA
DGFSRDLGVKFGATGRKKLKNETSAFGWGVNSGFGGGDKWEAQTKINLPVAAAANSISLVRAISSGALNLELSASESLSK
TKTLANPRVLTQNRKEAKIESGYEIPFTVTTASGGGNSTNTELKKAVLGLTVTPNITPDGQIIMTVKINKDSPAQCASGN
NTILCISTKSLNTQAMVENGGTLIVGGIYEENNGNTLTKVPLLGDIPVIGNLFKTRGKKTDRRELLIFITPRIIDTAGNS
LRY

Nucleotide


Download         Length: 2172 bp        

>NTDB_id=937116 V5G09_RS00495 WP_003698035.1 103612..105783(-) (pilQ) [Neisseria gonorrhoeae strain WHO_B_2024]
ATGAATACCAAACTGACAAAAATCATTTCCGGTCTCTTTGTCGCAACCGCCGCCTTTCAGACGGCATCGGCAGGAAACAT
TACAGACATCAAAGTTTCCTCCCTGCCCAACAAACAGAAAATCGTCAAAGTCAGCTTTGACAAAGAGATTGTCAACCCGA
CCGGCTTCGTAACCTCCTCACCGGCCCGCATCGCCTTGGACTTTGAACAAACCGGCATTTCCATGGATCAACAGGTACTC
GAATATGCCGATCCTCTGTTGAGCAAAATCAGTGCCGCACAAAACAGCAGCCGTGCGCGTCTGGTTCTGAATTTGAACAA
ACCGGGCCAATACAATACCGAAGTACGCGGGAACAAAGTTTGGATATTCATTAACGAATCGGACGATACCGTGTCCGCCC
CCGCCCGCCCAGCCGTAAAAGCCGCGCCTGCCGCACCGGCAAAACAACAGGCTGCCGCACCGTTTACCGAGTCCGTAGTA
TCCGTATCCGCACCGTTCAGCCCGGCAAAACAACAGGCAGCGGCATCGGCAAAACAAACCAATATCGATTTCCGCAAAGA
CGGCAAAAATGCCGGCATTATCGAATTGGCGGCATTGGGCTTTGCCGGGCAGCCCGACATCAGCCAACAGCACGACCACA
TCATCGTTACGCTGAAAAACCATACCCTGCCGACCGCGCTCCAACGCAGTTTGGATGTGGCAGACTTCAAAACACCGGTT
CAAAAGGTTACGCTGAAACGCCTCAATAACGACACCCAGCTGATTATCACAACAACCGGCAACTGGGAACTCGTCAACAA
ATCCGCCGCGCCCGGATACTTTACCTTCCAAGTCCTGCCGAAAAAACAAAACCTCGAGTCAGGCGGCGTGAACAATGCGC
CCAAAACCTTCACAGGCCGGAAAATCTCCCTTGACTTCCAAGATGTCGAAATCCGCACCATCCTGCAGATTTTGGCAAAA
GAATCCGGGATGAACATTGTTGCCAGCGACTCCGTCAGCGGCAAAATGACCCTCTCCCTCAAAGACGTACCTTGGGATCA
GGCTTTGGATTTGGTTATGCAGGCGCGCAACCTCGATATGCGCCAGCAAGGGAACATCGTCAACATCGCGCCCCGCGACG
AGCTGCTTGCCAAAGACAAAGCCTTCTTACAAGCGGAAAAAGACATTGCCGATCTGGGCGCGCTGTATTCCCAAAACTTC
CAATTGAAATACAAAAATGTGGAAGAATTCCGCAGCATCCTGCGTTTGGACAATGCCGACACGACCGGAAACCGCAACAC
GCTTGTCAGCGGCAGGGGCAGCGTGCTGATCGATCCCGCCACCAACACCCTGATTGTTACCGATACCCGCAGCGTCATCG
AAAAATTCCGCAAACTGATTGACGAATTGGACGTACCCGCGCAACAAGTGATGATTGAGGCGCGTATCGTCGAAGCGGCA
GACGGCTTCTCGCGCGATTTGGGCGTTAAGTTCGGCGCGACAGGCAGGAAAAAACTGAAAAATGAGACGAGCGCATTCGG
CTGGGGCGTGAACTCCGGCTTCGGGGGCGGCGATAAATGGGAGGCCCAAACCAAAATCAACCTGCCGGTTGCCGCTGCCG
CAAACAGCATTTCGCTGGTGCGCGCGATTTCCTCCGGCGCGTTGAATTTGGAATTGTCCGCATCCGAGTCGCTTTCAAAA
ACCAAAACGCTTGCCAATCCGCGCGTGCTGACCCAAAACCGCAAAGAGGCCAAAATCGAATCCGGTTACGAAATTCCTTT
TACCGTAACTACAGCCTCGGGCGGCGGCAACTCTACCAACACGGAACTCAAAAAAGCCGTCTTGGGGCTGACCGTTACGC
CGAACATCACGCCCGACGGACAAATCATCATGACCGTCAAAATCAACAAAGACTCGCCTGCACAATGTGCTTCAGGCAAC
AACACAATCCTATGTATTTCGACCAAAAGCCTGAATACGCAGGCTATGGTTGAAAACGGCGGCACTTTGATTGTCGGCGG
TATTTATGAAGAAAACAACGGCAATACGCTGACCAAAGTCCCCCTGTTGGGCGACATCCCCGTTATCGGCAACCTCTTTA
AAACACGCGGGAAAAAAACCGACCGCCGCGAACTGCTGATTTTCATTACCCCGAGGATTATAGATACGGCCGGCAACAGC
CTGCGCTATTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(325-370)

AMIN domain (AMIN)

(43-116)

Bacterial type II/III secretion system short domain (Secretin_N)

(398-469)

Bacterial type II and III secretion system protein (Secretin)

(555-715)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q5FAD2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Neisseria gonorrhoeae strain FA1090

100

100

1
  pilQ Neisseria meningitidis MC58

89.987

100

0.957
  pilQ Neisseria meningitidis M1080

90.802

100

0.956