Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   LLL8_RS05355 Genome accession   NZ_AP025700
Coordinates   1068887..1070209 (-) Length   440 a.a.
NCBI ID   WP_251900379.1    Uniprot ID   -
Organism   Lactococcus lactis strain L8     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1063887..1075209
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LLL8_RS05325 (LLL8_10320) - 1065030..1065998 (+) 969 WP_003130649.1 PhoH family protein -
  LLL8_RS05330 (LLL8_10330) - 1066056..1066535 (+) 480 WP_228248033.1 NUDIX hydrolase -
  LLL8_RS05335 (LLL8_10340) ybeY 1066687..1067175 (+) 489 WP_021722516.1 rRNA maturation RNase YbeY -
  LLL8_RS05340 (LLL8_10350) - 1067159..1067614 (+) 456 WP_003130653.1 diacylglycerol kinase -
  LLL8_RS05345 (LLL8_10360) - 1067628..1068206 (+) 579 WP_021722515.1 nucleotidyltransferase family protein -
  LLL8_RS05350 comFC 1068240..1068644 (-) 405 WP_021722514.1 ComF family protein Machinery gene
  LLL8_RS05355 (LLL8_10380) comFA 1068887..1070209 (-) 1323 WP_251900379.1 DEAD/DEAH box helicase Machinery gene
  LLL8_RS05360 (LLL8_10390) - 1070266..1070895 (+) 630 WP_003130660.1 YigZ family protein -
  LLL8_RS05365 (LLL8_10400) - 1071029..1071526 (+) 498 WP_003130662.1 VanZ family protein -
  LLL8_RS05370 (LLL8_10410) - 1071574..1072593 (+) 1020 WP_012897709.1 DUF475 domain-containing protein -
  LLL8_RS05375 (LLL8_10420) - 1072709..1073455 (+) 747 WP_058208415.1 bifunctional metallophosphatase/5'-nucleotidase -
  LLL8_RS05380 (LLL8_10430) - 1073748..1074395 (+) 648 WP_251900380.1 GIY-YIG nuclease family protein -

Sequence

Protein


Download         Length: 440 a.a.        Molecular weight: 50130.64 Da        Isoelectric Point: 10.1065

>NTDB_id=93686 LLL8_RS05355 WP_251900379.1 1068887..1070209(-) (comFA) [Lactococcus lactis strain L8]
MSTNQEKLFGRLLLKNDILQLIKSTDKISVSKIFSNFLLEAKVNPILGMTSISSNKIKCNRCGTVHIKNSVKLPIGVFYC
PSCIQLGRVRSDEFLYFLPQKNFPKKSYINWSGKLTENQKSISNALCQEINSHQQIIVQAVTGAGKTEMIYQVIEQILES
GGVVGLASPRIDVCLELHQRLSRDFSCKIPLLYHDGDSYFRAPLIIMTSHQLLRFKEAFDLLVIDEVDAFPFRDNEMLYV
AAEKARKIEGNLIYLTATSTDKLEKDIKKQKLYPLFLPRRFHNFPLVVPKFFWKNKFDKKLIEQRNSGFPLLIFAAEIEF
GQEFAKQLQLKFPKEKIASVASTTKDRLEIVKAFRNKEITILIATSILERGVTFPNVDVFVINSEHPNFTKSALIQMAGR
VGRSPERPTGLVSFFHYGKSKAMCQAVREIKKMNQLGGFS

Nucleotide


Download         Length: 1323 bp        

>NTDB_id=93686 LLL8_RS05355 WP_251900379.1 1068887..1070209(-) (comFA) [Lactococcus lactis strain L8]
ATGAGCACTAATCAAGAAAAGTTATTTGGCCGTTTATTATTAAAAAATGATATTTTACAACTTATAAAAAGTACTGACAA
GATTTCTGTCAGTAAAATTTTTAGTAATTTTTTGTTAGAAGCGAAGGTGAATCCAATTTTGGGAATGACTTCAATTTCTT
CCAATAAAATAAAATGCAACCGTTGTGGGACTGTTCATATAAAAAATTCTGTCAAACTTCCAATTGGTGTATTTTACTGT
CCAAGTTGTATTCAATTAGGTCGAGTCCGCTCCGATGAATTCTTGTACTTTCTGCCACAAAAGAATTTCCCAAAGAAATC
ATATATAAACTGGTCGGGAAAACTGACAGAGAATCAAAAATCAATTTCAAATGCCCTCTGTCAGGAAATTAATTCTCATC
AGCAAATAATTGTCCAAGCTGTGACTGGAGCTGGAAAAACTGAAATGATTTATCAAGTCATTGAACAAATTTTAGAAAGC
GGTGGGGTTGTTGGTCTAGCTAGTCCAAGAATTGATGTTTGTCTTGAACTTCATCAGCGATTATCACGTGATTTTTCCTG
TAAGATTCCACTCTTATATCATGATGGCGACAGCTATTTCCGAGCTCCATTAATAATAATGACCAGTCATCAGCTTTTAC
GTTTCAAGGAAGCTTTTGATTTGCTGGTTATTGATGAAGTTGATGCCTTTCCCTTTAGAGATAATGAAATGCTTTATGTT
GCGGCAGAAAAAGCAAGAAAAATAGAAGGAAATTTAATATATTTGACCGCAACTTCTACTGACAAACTTGAAAAAGATAT
AAAAAAGCAAAAACTCTATCCTTTGTTTCTCCCGCGTCGTTTTCACAATTTCCCTTTAGTGGTGCCTAAATTTTTTTGGA
AAAATAAATTTGATAAGAAATTAATTGAGCAAAGAAATAGTGGCTTTCCTCTTCTTATTTTTGCTGCTGAAATTGAATTT
GGACAAGAATTTGCAAAACAACTACAATTGAAATTTCCTAAAGAAAAAATTGCTTCCGTTGCTTCAACAACAAAAGATAG
ATTGGAAATTGTTAAAGCTTTTAGAAATAAAGAAATTACTATTTTAATAGCGACTTCAATTCTTGAACGGGGAGTCACTT
TTCCAAATGTTGATGTTTTTGTCATCAACAGTGAGCACCCAAACTTCACTAAATCCGCACTGATACAAATGGCTGGACGT
GTGGGTCGTAGTCCTGAACGTCCAACAGGCTTAGTTAGTTTTTTTCATTACGGAAAATCCAAAGCAATGTGTCAGGCAGT
TAGAGAAATCAAAAAAATGAATCAACTTGGAGGTTTTTCATGA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(300-403)

Type III restriction enzyme, res subunit (ResIII)

(114-227)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Lactococcus lactis subsp. cremoris KW2

74.25

90.909

0.675
  comFA/cflA Streptococcus mitis SK321

51.759

90.455

0.468
  comFA/cflA Streptococcus pneumoniae Rx1

51.759

90.455

0.468
  comFA/cflA Streptococcus pneumoniae D39

51.759

90.455

0.468
  comFA/cflA Streptococcus pneumoniae R6

51.759

90.455

0.468
  comFA/cflA Streptococcus pneumoniae TIGR4

51.759

90.455

0.468
  comFA/cflA Streptococcus mitis NCTC 12261

51.005

90.455

0.461
  comFA Bacillus subtilis subsp. subtilis str. 168

36.807

100

0.377

Multiple sequence alignment