Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   SAJP025_RS07750 Genome accession   NZ_AP025683
Coordinates   1618747..1619244 (-) Length   165 a.a.
NCBI ID   WP_001799534.1    Uniprot ID   -
Organism   Staphylococcus aureus strain JP025     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1613747..1624244
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SAJP025_RS07725 (SAJP025_14740) gcvPB 1613909..1615381 (-) 1473 WP_000202178.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  SAJP025_RS07730 (SAJP025_14750) gcvPA 1615374..1616720 (-) 1347 WP_029264507.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  SAJP025_RS07735 (SAJP025_14760) gcvT 1616740..1617831 (-) 1092 WP_000093347.1 glycine cleavage system aminomethyltransferase GcvT -
  SAJP025_RS07740 (SAJP025_14770) - 1617990..1618514 (-) 525 WP_001015125.1 shikimate kinase -
  SAJP025_RS07745 - 1618504..1618650 (-) 147 WP_001789879.1 hypothetical protein -
  SAJP025_RS07750 (SAJP025_14780) comGF 1618747..1619244 (-) 498 WP_001799534.1 competence type IV pilus minor pilin ComGF Machinery gene
  SAJP025_RS07755 (SAJP025_14790) comGE 1619162..1619461 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  SAJP025_RS07760 (SAJP025_14800) comGD 1619448..1619894 (-) 447 WP_001791207.1 competence type IV pilus minor pilin ComGD Machinery gene
  SAJP025_RS07765 (SAJP025_14810) comGC 1619872..1620183 (-) 312 WP_000472257.1 competence type IV pilus major pilin ComGC Machinery gene
  SAJP025_RS07770 (SAJP025_14820) comGB 1620197..1621267 (-) 1071 WP_000776412.1 competence type IV pilus assembly protein ComGB Machinery gene
  SAJP025_RS07775 (SAJP025_14830) comGA 1621239..1622213 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  SAJP025_RS07780 (SAJP025_14840) - 1622265..1622888 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  SAJP025_RS07785 (SAJP025_14850) - 1622885..1623214 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  SAJP025_RS07790 (SAJP025_14860) - 1623214..1624200 (-) 987 WP_000161314.1 ROK family glucokinase -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19210.00 Da        Isoelectric Point: 10.2192

>NTDB_id=93578 SAJP025_RS07750 WP_001799534.1 1618747..1619244(-) (comGF) [Staphylococcus aureus strain JP025]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVIAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=93578 SAJP025_RS07750 WP_001799534.1 1618747..1619244(-) (comGF) [Staphylococcus aureus strain JP025]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTAATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
TAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACAATTTATGTATAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(68-155)

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

98.182

100

0.982
  comGF Staphylococcus aureus MW2

96.97

100

0.97

Multiple sequence alignment