Detailed information    

experimental Experimentally validated

Overview

Name   dprA   Type   Machinery gene
Locus tag   Cj0634 Genome accession   NC_002163
Coordinates   596646..597419 (+) Length   257 a.a.
NCBI ID   YP_002344063.1    Uniprot ID   Q0PAN4
Organism   Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819     
Function   ssDNA binding; loading RecA onto ssDNA   
DNA processing

Function

C. jejuni expresses a DprA ortholog that functionally most closely resembles that of H. pylori and is involved in DNA transformation.


Genomic Context

Location: 591646..602419
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Cj0631c - 592495..594429 (-) 1935 YP_002344060.1 ribonuclease -
  Cj0632 ilvC 594548..595570 (+) 1023 YP_002344061.1 ketol-acid reductoisomerase -
  Cj0633 - 595574..596656 (+) 1083 YP_002344062.1 periplasmic protein -
  Cj0634 dprA 596646..597419 (+) 774 YP_002344063.1 DNA processing protein A Machinery gene
  Cj0635 - 597416..597799 (+) 384 YP_002344064.1 Holliday junction resolvase-like protein -
  Cj0636 - 597828..598667 (+) 840 YP_002344065.1 NOL1/NOP2/sun family protein -
  Cj0637c mrsA 598843..599340 (-) 498 YP_002344066.1 methionine sulfoxide reductase A -
  Cj0638c ppa 599381..599899 (-) 519 YP_002344067.1 inorganic pyrophosphatase -
  Cj0639c adk 599909..600487 (-) 579 YP_002344068.1 adenylate kinase -
  Cj0640c aspS 600484..602235 (-) 1752 YP_002344069.1 aspartate--tRNA ligase -

Sequence

Protein


Download         Length: 257 a.a.        Molecular weight: 28774.64 Da        Isoelectric Point: 7.6510

>NTDB_id=1239 Cj0634 YP_002344063.1 596646..597419(+) (dprA) [Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819]
MASEILPDEFLELFKDLKKPPKKLHYKGNLSLLKQDKIAIIGSRRMSVYTKNCVFSLASMLKNAHLCVVSGGALGVDITA
SMAAMPNTIGIFANGLDQIYPRTNEKIIKQIYENALALSEYEDDYLPKNYDFLLRNRLVIALSKAVVVAQADIKSGSMQS
AKLALELNKPLYVLPQRLGESTATNLLLKENKAKLICDFKEFVSEFASIDMNQDEFLEFCKKGVSVDEALKIYGQKVYEY
ELEGKISIEGLFIRVLA

Nucleotide


Download         Length: 774 bp        

>NTDB_id=1239 Cj0634 YP_002344063.1 596646..597419(+) (dprA) [Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819]
ATGGCAAGTGAAATTTTGCCTGATGAATTTTTAGAACTTTTTAAAGATTTAAAAAAGCCTCCTAAAAAATTGCACTATAA
AGGAAATTTATCTTTATTAAAACAAGATAAAATCGCTATTATAGGTTCAAGGCGCATGAGTGTTTATACGAAAAATTGTG
TTTTTTCCTTAGCGAGCATGCTTAAAAATGCTCATCTTTGTGTGGTAAGTGGCGGTGCTTTGGGTGTGGATATCACCGCA
AGTATGGCGGCTATGCCAAATACTATAGGTATTTTTGCCAATGGTTTAGATCAAATTTATCCAAGAACTAATGAAAAAAT
CATTAAGCAAATTTATGAAAATGCTTTAGCTTTAAGCGAATACGAAGATGATTATTTGCCAAAAAATTATGACTTTTTGC
TAAGAAATCGTTTGGTTATTGCTTTAAGTAAGGCTGTTGTGGTTGCACAAGCAGATATAAAAAGTGGTTCTATGCAAAGT
GCAAAACTTGCTTTAGAACTCAATAAGCCTTTATATGTTTTACCGCAAAGACTAGGAGAAAGCACAGCAACTAATTTGCT
TTTAAAGGAAAATAAAGCAAAGTTAATTTGTGATTTTAAAGAATTTGTAAGTGAATTTGCAAGTATAGATATGAATCAAG
ATGAATTTTTAGAGTTTTGTAAAAAAGGTGTGAGTGTTGATGAGGCTTTAAAAATTTATGGACAAAAAGTTTATGAATAT
GAACTTGAGGGAAAAATTTCCATAGAAGGGCTTTTTATAAGGGTTTTAGCATGA

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(9-197)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q0PAN4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Helicobacter pylori 26695

48.75

93.385

0.455

Multiple sequence alignment    



References

[1] Tohru Takata et al. (2005) Role of dprA in transformation of Campylobacter jejuni. FEMS Microbiology Letters 252(1):161-8. [PMID: 16194595]