Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   V3H91_RS13695 Genome accession   NZ_CP144281
Coordinates   2718545..2719159 (-) Length   204 a.a.
NCBI ID   WP_015387983.1    Uniprot ID   A0A6A8LHQ3
Organism   Bacillus velezensis strain SEC-024A     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2713545..2724159
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V3H91_RS13665 rpsT 2713845..2714111 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  V3H91_RS13670 holA 2714128..2715169 (-) 1042 Protein_2649 DNA polymerase III subunit delta -
  V3H91_RS13675 - 2715209..2715363 (-) 155 Protein_2650 hypothetical protein -
  V3H91_RS13680 - 2715404..2715538 (+) 135 WP_003152870.1 YqzM family protein -
  V3H91_RS13685 comEC 2715557..2717908 (-) 2352 WP_107994305.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  V3H91_RS13690 - 2717909..2718478 (-) 570 WP_003152868.1 ComE operon protein 2 -
  V3H91_RS13695 comEA 2718545..2719159 (-) 615 WP_015387983.1 helix-hairpin-helix domain-containing protein Machinery gene
  V3H91_RS13700 comER 2719218..2720039 (+) 822 WP_014305449.1 late competence protein ComER -
  V3H91_RS13705 - 2720108..2720845 (-) 738 WP_107994304.1 class I SAM-dependent DNA methyltransferase -
  V3H91_RS13710 rsfS 2720842..2721198 (-) 357 WP_003152864.1 ribosome silencing factor -
  V3H91_RS13715 yqeK 2721195..2721776 (-) 582 WP_107994303.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  V3H91_RS13720 - 2721766..2722335 (-) 570 WP_003152860.1 nicotinate-nucleotide adenylyltransferase -
  V3H91_RS13725 yhbY 2722346..2722636 (-) 291 WP_003152858.1 ribosome assembly RNA-binding protein YhbY -
  V3H91_RS13730 aroE 2722630..2723472 (-) 843 WP_003152856.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22255.41 Da        Isoelectric Point: 5.5211

>NTDB_id=933376 V3H91_RS13695 WP_015387983.1 2718545..2719159(-) (comEA) [Bacillus velezensis strain SEC-024A]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=933376 V3H91_RS13695 WP_015387983.1 2718545..2719159(-) (comEA) [Bacillus velezensis strain SEC-024A]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGTTGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6A8LHQ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

58.854

94.118

0.554
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

35.545

100

0.368
  comEA/celA/cilE Streptococcus mitis SK321

35.071

100

0.363