Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   MWM02_RS05715 Genome accession   NZ_AP025627
Coordinates   1107744..1108808 (+) Length   354 a.a.
NCBI ID   WP_244403137.1    Uniprot ID   -
Organism   Parageobacillus sp. KH3-4     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1102744..1113808
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MWM02_RS05685 (PspKH34_10860) - 1103340..1103651 (+) 312 WP_064549835.1 MTH1187 family thiamine-binding protein -
  MWM02_RS05690 (PspKH34_10870) - 1103684..1103860 (-) 177 WP_064549836.1 DUF2759 domain-containing protein -
  MWM02_RS05695 (PspKH34_10880) - 1104215..1104847 (+) 633 WP_064549837.1 MBL fold metallo-hydrolase -
  MWM02_RS05700 (PspKH34_10890) - 1105221..1106354 (+) 1134 WP_244403136.1 SAM-dependent methyltransferase -
  MWM02_RS05705 (PspKH34_10900) - 1106403..1106645 (-) 243 WP_008879854.1 DUF2626 family protein -
  MWM02_RS05710 (PspKH34_10910) - 1106847..1107545 (-) 699 WP_064549839.1 helix-turn-helix domain-containing protein -
  MWM02_RS05715 (PspKH34_10920) comGA 1107744..1108808 (+) 1065 WP_244403137.1 competence type IV pilus ATPase ComGA Machinery gene
  MWM02_RS05720 (PspKH34_10930) comGB 1108801..1109832 (+) 1032 WP_064549894.1 competence type IV pilus assembly protein ComGB -
  MWM02_RS05725 (PspKH34_10940) comGC 1109845..1110141 (+) 297 WP_064549895.1 competence type IV pilus major pilin ComGC Machinery gene
  MWM02_RS05730 (PspKH34_10950) comGD 1110125..1110568 (+) 444 WP_244403139.1 competence type IV pilus minor pilin ComGD -
  MWM02_RS05735 (PspKH34_10960) comGE 1110552..1110875 (+) 324 WP_064549897.1 competence type IV pilus minor pilin ComGE -
  MWM02_RS05740 (PspKH34_10970) comGF 1110872..1111297 (+) 426 WP_244403140.1 competence type IV pilus minor pilin ComGF -
  MWM02_RS05745 (PspKH34_10980) comGG 1111315..1111704 (+) 390 WP_064549899.1 competence type IV pilus minor pilin ComGG -
  MWM02_RS05750 (PspKH34_10990) - 1111963..1112142 (+) 180 WP_064549900.1 YqzE family protein -
  MWM02_RS05755 (PspKH34_11000) - 1112211..1113002 (-) 792 WP_064549901.1 YqhG family protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 39610.05 Da        Isoelectric Point: 8.7676

>NTDB_id=93288 MWM02_RS05715 WP_244403137.1 1107744..1108808(+) (comGA) [Parageobacillus sp. KH3-4]
MNEIEYLADRLIKEASVLHVSDIHIVPRKDDAAVRFRLDGLLMEKEVLTKEICERLITHFKFLAGMDIGERRRPQSGAME
TKQQGEIIHLRLSTLPTSYDESLVIRLLPQNLFLPLSQLSLFANAAKTLLSLFQQPQGLIIFTGPAGSGKTTTLYTLLRV
CQSEWNRNVITLEDPVEKRIDNILQVQINEKAGITYANGLKAALRHDPNVVMIGEIRDAETAKIAVRSALTGHLIASTMH
TKDAVGAIYRLREFGIPLHDIEQTLLAVTAQRLVDLVCPFCGEHCSLFCRKYRKIRRAAVHELLHGDALSGAIQSVQSGR
KTYHYYTLQNAIRKGIALGFLPPRLLCAKGGENE

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=93288 MWM02_RS05715 WP_244403137.1 1107744..1108808(+) (comGA) [Parageobacillus sp. KH3-4]
ATGAACGAAATCGAATATCTTGCTGATCGCCTCATCAAAGAAGCGAGTGTGCTTCATGTGTCTGATATTCACATCGTTCC
GCGCAAAGACGATGCAGCAGTGCGTTTCCGGCTTGATGGATTGCTGATGGAAAAAGAAGTGCTCACAAAAGAAATATGCG
AGCGCCTTATTACCCATTTTAAGTTTTTAGCTGGAATGGACATTGGAGAACGTCGCCGGCCGCAAAGCGGAGCGATGGAA
ACGAAGCAACAAGGAGAAATCATTCACTTGCGCCTTTCCACATTGCCGACATCTTACGATGAAAGCCTCGTTATTCGGCT
TCTTCCGCAAAACTTGTTTCTTCCTCTATCACAACTTTCTTTATTCGCCAATGCCGCAAAAACGTTACTTTCCCTTTTTC
AGCAACCTCAAGGGCTAATCATTTTCACAGGACCAGCCGGTTCAGGAAAGACGACGACATTGTATACGTTGCTGCGCGTC
TGCCAATCCGAATGGAACCGCAATGTCATCACATTGGAAGATCCCGTTGAAAAGCGAATCGACAATATATTGCAAGTGCA
AATCAATGAGAAAGCAGGAATCACATATGCAAACGGCTTAAAAGCGGCTTTGCGGCACGACCCGAACGTCGTTATGATCG
GTGAAATCCGGGATGCTGAGACGGCAAAAATCGCGGTACGTTCCGCGCTGACGGGACATTTGATAGCGTCAACGATGCAT
ACGAAAGACGCTGTTGGGGCGATTTACCGTTTGCGTGAATTCGGGATTCCGCTTCACGATATTGAGCAAACGCTGCTCGC
CGTGACAGCGCAGCGGCTTGTCGATTTAGTCTGTCCGTTTTGCGGAGAACATTGCTCGCTATTTTGCCGCAAATATCGCA
AAATTCGCCGCGCCGCCGTTCACGAACTGCTTCATGGGGACGCGCTGTCTGGCGCAATTCAATCCGTACAAAGCGGGAGA
AAAACGTATCACTACTATACGCTGCAAAACGCTATTCGAAAAGGAATTGCTCTCGGATTTTTGCCGCCGCGCCTTCTTTG
CGCCAAGGGGGGAGAAAATGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

56.14

96.61

0.542
  pilB Haemophilus influenzae 86-028NP

42.244

85.593

0.362
  pilB Glaesserella parasuis strain SC1401

41.558

87.006

0.362

Multiple sequence alignment