Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   V1565_RS21730 Genome accession   NZ_CP143691
Coordinates   4428700..4429680 (-) Length   326 a.a.
NCBI ID   WP_045332866.1    Uniprot ID   A0A837FII0
Organism   Enterobacter hormaechei subsp. xiangfangensis strain ECC-181     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4423700..4434680
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V1565_RS21700 (V1565_21680) - 4424581..4425078 (+) 498 WP_126524571.1 SprT family zinc-dependent metalloprotease -
  V1565_RS21705 (V1565_21685) endA 4425173..4425880 (+) 708 WP_003860034.1 deoxyribonuclease I -
  V1565_RS21710 (V1565_21690) rsmE 4425932..4426663 (+) 732 WP_017382923.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  V1565_RS21715 (V1565_21695) gshB 4426691..4427638 (+) 948 WP_003860032.1 glutathione synthase -
  V1565_RS21720 (V1565_21700) - 4427713..4428273 (+) 561 WP_022649353.1 YqgE/AlgH family protein -
  V1565_RS21725 (V1565_21705) ruvX 4428273..4428689 (+) 417 WP_006811925.1 Holliday junction resolvase RuvX -
  V1565_RS21730 (V1565_21710) pilT 4428700..4429680 (-) 981 WP_045332866.1 type IV pilus twitching motility protein PilT Machinery gene
  V1565_RS21735 (V1565_21715) - 4429698..4430399 (+) 702 WP_032610102.1 YggS family pyridoxal phosphate-dependent enzyme -
  V1565_RS21740 (V1565_21720) - 4430421..4430987 (+) 567 WP_003860024.1 YggT family protein -
  V1565_RS21745 (V1565_21725) yggU 4430984..4431280 (+) 297 WP_003860023.1 DUF167 family protein YggU -
  V1565_RS21750 (V1565_21730) - 4431284..4431877 (+) 594 WP_022651831.1 XTP/dITP diphosphatase -
  V1565_RS21755 (V1565_21735) hemW 4431870..4433018 (+) 1149 WP_126524573.1 radical SAM family heme chaperone HemW -
  V1565_RS21760 (V1565_21740) - 4433075..4433437 (+) 363 WP_022651832.1 endonuclease domain-containing protein -
  V1565_RS21765 (V1565_21745) - 4433504..4434220 (-) 717 WP_003862419.1 DUF2884 domain-containing protein -
  V1565_RS21770 (V1565_21750) - 4434277..4434603 (-) 327 WP_003862421.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35749.75 Da        Isoelectric Point: 6.2344

>NTDB_id=930702 V1565_RS21730 WP_045332866.1 4428700..4429680(-) (pilT) [Enterobacter hormaechei subsp. xiangfangensis strain ECC-181]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRSGRLEPAPFPPPDVEALLKAWLNDEQQGAWWANGQVDFAVTLADRQRLRG
SAFKHMHGVSIALRLLPLTCPQLSALGVPRAIPELLSNDNGLILVTGATGSGKSTTLAAMVDFLNHQTDGHILTLEDPVE
FMYQSERCLIQQREIGLHSPSFAEALRSALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLRQDVQGGRVALYELLVNTSAAANLIREGKTWQLPGIIQTGQQAGMQNFEQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=930702 V1565_RS21730 WP_045332866.1 4428700..4429680(-) (pilT) [Enterobacter hormaechei subsp. xiangfangensis strain ECC-181]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCACCACCGCG
CTGGCGCAGGTCAGGCCGTCTTGAACCTGCGCCGTTTCCGCCCCCGGATGTGGAGGCATTATTAAAAGCGTGGCTCAACG
ACGAACAGCAGGGCGCCTGGTGGGCAAATGGGCAGGTGGATTTTGCCGTTACCCTCGCAGACCGTCAGCGGCTGCGCGGC
AGTGCGTTTAAGCATATGCACGGCGTTTCGATCGCGCTGCGCCTGTTGCCGCTGACGTGCCCGCAGCTCTCTGCGTTAGG
TGTGCCGCGCGCGATCCCGGAGCTTTTGTCCAATGACAATGGCCTGATTCTGGTCACCGGCGCCACCGGCAGCGGGAAAT
CGACCACCCTGGCCGCGATGGTCGATTTCCTCAATCACCAGACGGACGGGCATATTCTGACCCTGGAAGATCCGGTGGAG
TTTATGTACCAGAGTGAACGTTGCCTGATCCAGCAGCGGGAGATAGGCCTGCACAGCCCGTCCTTTGCCGAGGCCCTGCG
CAGCGCGCTGCGTGAAGATCCGGATGTGATTCTGCTTGGTGAGCTGCGCGACAGCGAGACGATACGCCTGGCGCTGACGG
CGGCGGAAACCGGCCATCTGGTGTTGGCCACGCTGCACACGCGCGGTGCGGCGCAGGCGATCGAGCGCCTGGTCGATACC
TTTCCGGCGCAGGAGAAAGATCCGGTGCGTAATCAGCTGGCGGGAAGCCTGCGTGCGGTTCTGGCGCAGAAGCTGCGTCA
GGATGTCCAGGGCGGGCGCGTGGCGCTGTATGAGCTACTGGTGAATACGTCGGCGGCGGCAAACCTGATCCGCGAAGGCA
AAACCTGGCAACTGCCGGGCATTATTCAAACGGGGCAGCAGGCGGGGATGCAGAACTTTGAGCAGAGTCTGGCGGAGCGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(75-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A837FII0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

50.459

100

0.506
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.459

100

0.506
  pilT Legionella pneumophila strain ERS1305867

48.758

98.773

0.482
  pilT Legionella pneumophila strain Lp02

48.758

98.773

0.482
  pilT Pseudomonas stutzeri DSM 10701

47.706

100

0.479
  pilT Neisseria meningitidis 8013

47.256

100

0.475
  pilT Acinetobacter baumannii strain A118

47.095

100

0.472
  pilT Acinetobacter baumannii D1279779

47.095

100

0.472
  pilT Neisseria gonorrhoeae MS11

46.951

100

0.472
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Pseudomonas aeruginosa PAK

46.789

100

0.469
  pilT Acinetobacter nosocomialis M2

47.5

98.16

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.653

99.08

0.433
  pilU Pseudomonas stutzeri DSM 10701

37.879

100

0.383
  pilU Vibrio cholerae strain A1552

38.509

98.773

0.38
  pilB Haemophilus influenzae 86-028NP

33.146

100

0.362