Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   V1M48_RS11475 Genome accession   NZ_CP143559
Coordinates   336410..337003 (+) Length   197 a.a.
NCBI ID   WP_005451500.1    Uniprot ID   A0A9X3S2A2
Organism   Vibrio harveyi strain WHSS0915     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 331410..342003
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V1M48_RS11460 (V1M48_11460) - 332093..334663 (-) 2571 WP_199479218.1 penicillin-binding protein 1A -
  V1M48_RS11465 (V1M48_11465) pilM 334842..335858 (+) 1017 WP_029789968.1 type IV pilus assembly protein PilM Machinery gene
  V1M48_RS11470 (V1M48_11470) pilN 335842..336417 (+) 576 WP_005451499.1 PilN domain-containing protein Machinery gene
  V1M48_RS11475 (V1M48_11475) pilO 336410..337003 (+) 594 WP_005451500.1 type 4a pilus biogenesis protein PilO Machinery gene
  V1M48_RS11480 (V1M48_11480) pilP 336993..337508 (+) 516 WP_005451501.1 pilus assembly protein PilP Machinery gene
  V1M48_RS11485 (V1M48_11485) pilQ 337543..339294 (+) 1752 WP_005451505.1 type IV pilus secretin PilQ Machinery gene
  V1M48_RS11490 (V1M48_11490) aroK 339484..340002 (+) 519 WP_005451507.1 shikimate kinase AroK -
  V1M48_RS11495 (V1M48_11495) aroB 340034..341134 (+) 1101 WP_009697563.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 23020.58 Da        Isoelectric Point: 4.8068

>NTDB_id=929670 V1M48_RS11475 WP_005451500.1 336410..337003(+) (pilO) [Vibrio harveyi strain WHSS0915]
MVNWQDLEMDEIAEWPLVPQIVVILLLVVLIQGLGYWFYLKPEQEQLNNLIQEEQTLKVALRIKANKVAALPQLQSQLDE
LTTRYELLLQQLPAQKELATMLASVNELGIDNKLTFTRIDWGDKQSEHFLYRLPLDIELTGDFHDIGRFSQAIATLPRII
TFKDVTWQRVSQESETLHFRVKANTYQFKPEVKKDEK

Nucleotide


Download         Length: 594 bp        

>NTDB_id=929670 V1M48_RS11475 WP_005451500.1 336410..337003(+) (pilO) [Vibrio harveyi strain WHSS0915]
ATGGTTAATTGGCAAGATCTAGAAATGGATGAGATCGCTGAGTGGCCATTGGTGCCTCAGATAGTAGTGATCTTGTTGTT
AGTTGTGCTGATTCAAGGCCTTGGTTATTGGTTTTATTTAAAGCCAGAGCAAGAACAGCTGAATAACCTTATTCAAGAAG
AACAAACCTTAAAAGTTGCGTTACGGATCAAAGCGAACAAAGTCGCTGCTTTGCCTCAATTGCAATCGCAACTTGATGAG
CTTACTACTCGATACGAATTGCTACTGCAACAACTGCCTGCACAGAAAGAGCTGGCAACCATGTTGGCTTCGGTGAATGA
ACTCGGAATTGATAATAAGTTGACGTTCACCCGAATCGACTGGGGTGATAAGCAATCAGAACACTTCCTTTATCGTTTAC
CGCTCGACATTGAGTTGACAGGGGATTTTCATGATATCGGTCGTTTCTCCCAAGCGATTGCGACGTTGCCACGCATCATC
ACTTTTAAAGACGTGACTTGGCAGCGTGTCAGCCAAGAAAGTGAAACTCTGCACTTTCGTGTGAAAGCAAACACTTACCA
GTTCAAACCAGAGGTGAAGAAAGATGAGAAGTAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(47-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

93.158

96.447

0.898
  pilO Vibrio cholerae strain A1552

71.066

100

0.711