Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   V1499_RS14745 Genome accession   NZ_CP143543
Coordinates   2913639..2914724 (-) Length   361 a.a.
NCBI ID   WP_409270647.1    Uniprot ID   -
Organism   Neobacillus sp. SCS-31     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2908639..2919724
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V1499_RS14700 (V1499_14700) - 2909133..2909924 (+) 792 WP_409270639.1 YqhG family protein -
  V1499_RS14705 (V1499_14705) - 2909967..2910149 (-) 183 WP_316569625.1 YqzE family protein -
  V1499_RS14710 (V1499_14710) - 2910176..2910697 (-) 522 WP_409270640.1 shikimate kinase -
  V1499_RS14715 (V1499_14715) comGG 2910709..2911092 (-) 384 WP_409270641.1 competence type IV pilus minor pilin ComGG -
  V1499_RS14720 (V1499_14720) comGF 2911092..2911565 (-) 474 WP_409270642.1 competence type IV pilus minor pilin ComGF -
  V1499_RS14725 (V1499_14725) - 2911516..2911848 (-) 333 WP_409270643.1 type II secretion system protein -
  V1499_RS14730 (V1499_14730) comGD 2911832..2912269 (-) 438 WP_409270644.1 competence type IV pilus minor pilin ComGD -
  V1499_RS14735 (V1499_14735) comGC 2912269..2912607 (-) 339 WP_409270645.1 competence type IV pilus major pilin ComGC -
  V1499_RS14740 (V1499_14740) comGB 2912621..2913652 (-) 1032 WP_409270646.1 competence type IV pilus assembly protein ComGB -
  V1499_RS14745 (V1499_14745) comGA 2913639..2914724 (-) 1086 WP_409270647.1 competence type IV pilus ATPase ComGA Machinery gene
  V1499_RS14750 (V1499_14750) - 2915069..2915770 (+) 702 WP_409270648.1 helix-turn-helix transcriptional regulator -
  V1499_RS14755 (V1499_14755) - 2915856..2916098 (+) 243 WP_316572034.1 DUF2626 domain-containing protein -
  V1499_RS14760 (V1499_14760) - 2916667..2917755 (-) 1089 WP_409270649.1 class I SAM-dependent methyltransferase -
  V1499_RS14765 (V1499_14765) - 2917792..2918433 (-) 642 WP_409270650.1 MBL fold metallo-hydrolase -
  V1499_RS14770 (V1499_14770) - 2918594..2918776 (+) 183 WP_316572031.1 DUF2759 domain-containing protein -

Sequence

Protein


Download         Length: 361 a.a.        Molecular weight: 40138.25 Da        Isoelectric Point: 7.8004

>NTDB_id=929468 V1499_RS14745 WP_409270647.1 2913639..2914724(-) (comGA) [Neobacillus sp. SCS-31]
MVFSIASAIETLADRIIANAVEYHATDIHILPRKEDTLIQFRITNKLSPRMSLPKEECDRLISHFKFTAHMDIGERRRPQ
SGAVFAEVNGRSFGLRLSTLPSNNRESMVIRLLPQHDKYSFDRLSLFPGMARKLLNLLNHSHGMIIFTGPTGSGKTTTLY
SLLNETASKFHRSVITLEDPIEKECGTVLQVQVNEKAGVTYAAGLKAILRHDPDIIMVGEIRDAETARVAVRAAMTGHLV
LTTMHTRDSPGAIHRLHEFGVNWLEVEQTLVAVTAQRIVELICPYCSGECSPFCHSSGVRKRASVFELLSGKALEAAILS
SRGETVKVEYESLGKAILKGIALGYIKESEYERLVFSHEEA

Nucleotide


Download         Length: 1086 bp        

>NTDB_id=929468 V1499_RS14745 WP_409270647.1 2913639..2914724(-) (comGA) [Neobacillus sp. SCS-31]
GTGGTGTTTTCCATTGCTAGTGCCATAGAAACCCTCGCAGACAGAATTATCGCGAATGCTGTCGAGTATCATGCAACAGA
TATCCACATCCTCCCCCGTAAAGAAGATACGCTAATCCAATTCCGTATTACAAACAAACTAAGCCCTCGGATGTCCCTTC
CAAAGGAAGAATGCGACAGGCTCATTTCCCATTTTAAATTTACCGCCCATATGGATATTGGTGAGCGGCGAAGGCCCCAG
AGCGGCGCTGTTTTCGCCGAAGTCAATGGCCGTTCATTCGGGCTGCGGCTGTCAACCCTTCCGTCGAATAACCGAGAAAG
CATGGTCATCAGGCTCCTGCCCCAACATGACAAATATTCCTTCGACCGATTGTCCCTCTTTCCGGGTATGGCAAGAAAAT
TGCTGAACCTGCTCAATCATTCCCATGGCATGATTATTTTCACCGGGCCAACGGGGAGCGGCAAAACCACCACCCTTTAT
TCCCTTCTTAATGAAACAGCGAGTAAATTCCATCGAAGCGTAATTACCCTTGAGGATCCAATTGAAAAGGAATGCGGTAC
CGTCCTGCAGGTTCAAGTGAATGAAAAAGCAGGAGTAACGTATGCCGCTGGGCTGAAAGCGATTCTAAGGCATGATCCAG
ATATTATCATGGTCGGGGAAATCCGTGATGCTGAAACAGCCAGGGTGGCGGTGAGGGCCGCAATGACCGGGCATCTCGTA
CTTACAACAATGCACACAAGAGACTCGCCGGGGGCAATCCATCGGCTTCATGAGTTTGGGGTGAACTGGCTTGAGGTTGA
GCAAACGCTTGTCGCCGTTACCGCCCAGCGGATTGTCGAATTGATCTGTCCTTATTGCAGCGGGGAATGTTCCCCTTTCT
GCCATTCGAGCGGGGTGAGGAAAAGGGCGAGCGTCTTTGAGCTTCTTTCCGGAAAAGCCCTTGAAGCGGCCATTCTATCC
TCGAGGGGAGAAACGGTAAAAGTGGAATACGAATCATTAGGCAAGGCAATCCTGAAGGGGATTGCACTCGGTTATATTAA
AGAATCGGAATATGAAAGGCTGGTATTTTCCCATGAGGAAGCGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(16-280)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

55.775

98.338

0.548
  pilB Glaesserella parasuis strain SC1401

36.975

98.892

0.366
  pilB Haemophilus influenzae 86-028NP

37.429

96.953

0.363
  ctsE Campylobacter jejuni subsp. jejuni 81-176

37.901

95.014

0.36