Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilW   Type   Machinery gene
Locus tag   MWM05_RS10625 Genome accession   NZ_AP025593
Coordinates   1964785..1965729 (+) Length   314 a.a.
NCBI ID   WP_244362606.1    Uniprot ID   -
Organism   Thermus brockianus strain SNM4-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1959785..1970729
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MWM05_RS10600 (TbrSNM41_20910) - 1960674..1961183 (+) 510 WP_071677091.1 DUF5317 domain-containing protein -
  MWM05_RS10605 (TbrSNM41_20920) - 1961253..1962257 (+) 1005 WP_071677090.1 homoisocitrate dehydrogenase -
  MWM05_RS10610 (TbrSNM41_20930) pilM 1962450..1963583 (+) 1134 WP_244362604.1 type IV pilus assembly protein PilM Machinery gene
  MWM05_RS10615 (TbrSNM41_20940) pilN 1963576..1964199 (+) 624 WP_071677088.1 flagellar protein FliT Machinery gene
  MWM05_RS10620 (TbrSNM41_20950) pilO 1964189..1964788 (+) 600 WP_071677935.1 type 4a pilus biogenesis protein PilO Machinery gene
  MWM05_RS10625 (TbrSNM41_20960) pilW 1964785..1965729 (+) 945 WP_244362606.1 competence protein Machinery gene
  MWM05_RS10630 (TbrSNM41_20970) pilQ 1965741..1968017 (+) 2277 WP_244362608.1 secretin N-terminal domain-containing protein Machinery gene
  MWM05_RS10635 (TbrSNM41_20980) aroC 1968071..1969222 (+) 1152 WP_244362610.1 chorismate synthase -
  MWM05_RS10640 (TbrSNM41_20990) - 1969239..1969793 (+) 555 WP_244362612.1 shikimate kinase -

Sequence

Protein


Download         Length: 314 a.a.        Molecular weight: 31924.96 Da        Isoelectric Point: 4.6879

>NTDB_id=92943 MWM05_RS10625 WP_244362606.1 1964785..1965729(+) (pilW) [Thermus brockianus strain SNM4-1]
MKETLARLAQAWQSLPQSTKLLLAGLLLVLAISLWYVGFYLPAQAPVAAETPQAPAEETPKALEAPPIPPLAESAPSQPS
PPTPSPAPTAPQASIKAETTPLPLPQAKQEAPPPNPFVPLVVEVPATAAPPLPSPTPTPAIRPQPVPTGAPVRVSQGTPL
PTPQVATAPRPLPGTSGALPAPKVLTPTPKAQVAQAPVEASVAPAPPTALVEAPLSQVSQEAPPTPGQAPEGPAKTPLEA
LVEEKGLKLSGTLLGPVSVAILESKEGYLVLPVGSPIPGTEAVVRRIEGERITLALKEETLELAVSQAQAGGGQ

Nucleotide


Download         Length: 945 bp        

>NTDB_id=92943 MWM05_RS10625 WP_244362606.1 1964785..1965729(+) (pilW) [Thermus brockianus strain SNM4-1]
GTGAAGGAAACCCTCGCGCGCCTCGCCCAGGCGTGGCAAAGCCTGCCCCAGTCCACCAAACTCCTCTTGGCGGGTTTGCT
CCTGGTGCTGGCCATCAGCTTGTGGTACGTGGGCTTCTACCTGCCCGCCCAGGCGCCGGTGGCGGCGGAAACGCCCCAAG
CCCCCGCTGAGGAAACGCCGAAGGCCCTCGAGGCCCCTCCCATCCCTCCTTTGGCCGAATCCGCCCCCAGCCAACCCTCC
CCGCCCACCCCTTCCCCAGCGCCCACCGCCCCCCAAGCCTCCATCAAAGCGGAGACCACCCCTCTCCCCCTGCCCCAAGC
CAAACAGGAAGCCCCACCCCCTAACCCCTTCGTACCCCTGGTGGTGGAGGTTCCGGCCACGGCGGCCCCGCCCCTCCCCT
CGCCCACCCCCACCCCAGCCATCCGGCCCCAGCCGGTGCCCACGGGCGCCCCGGTGCGCGTGAGCCAGGGCACCCCTTTG
CCCACGCCCCAGGTGGCCACCGCCCCAAGGCCCCTGCCCGGGACCTCCGGGGCCCTTCCCGCCCCCAAGGTGCTCACCCC
CACCCCCAAGGCCCAGGTAGCCCAAGCCCCGGTGGAGGCCAGCGTGGCCCCGGCCCCACCCACCGCCCTCGTGGAAGCGC
CTTTATCCCAAGTTTCCCAGGAGGCTCCGCCCACGCCGGGCCAAGCCCCGGAGGGCCCCGCCAAGACCCCCCTCGAGGCC
CTGGTGGAGGAGAAGGGGCTTAAGCTCTCCGGTACCCTTTTGGGCCCGGTAAGCGTGGCCATCCTGGAGAGCAAGGAGGG
CTACCTGGTCCTGCCTGTGGGTAGCCCCATCCCCGGGACCGAGGCGGTGGTGCGCCGTATAGAAGGCGAGCGCATCACCT
TAGCCCTGAAAGAGGAAACGTTAGAACTGGCAGTTTCGCAAGCGCAAGCGGGAGGTGGTCAGTGA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilW Thermus thermophilus HB27

53.894

100

0.551

Multiple sequence alignment