Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   MWM05_RS10610 Genome accession   NZ_AP025593
Coordinates   1962450..1963583 (+) Length   377 a.a.
NCBI ID   WP_244362604.1    Uniprot ID   -
Organism   Thermus brockianus strain SNM4-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1957450..1968583
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MWM05_RS10595 (TbrSNM41_20900) - 1959265..1960638 (+) 1374 WP_420833573.1 HD-GYP domain-containing protein -
  MWM05_RS10600 (TbrSNM41_20910) - 1960674..1961183 (+) 510 WP_071677091.1 DUF5317 domain-containing protein -
  MWM05_RS10605 (TbrSNM41_20920) - 1961253..1962257 (+) 1005 WP_071677090.1 homoisocitrate dehydrogenase -
  MWM05_RS10610 (TbrSNM41_20930) pilM 1962450..1963583 (+) 1134 WP_244362604.1 type IV pilus assembly protein PilM Machinery gene
  MWM05_RS10615 (TbrSNM41_20940) pilN 1963576..1964199 (+) 624 WP_071677088.1 flagellar protein FliT Machinery gene
  MWM05_RS10620 (TbrSNM41_20950) pilO 1964189..1964788 (+) 600 WP_071677935.1 type 4a pilus biogenesis protein PilO Machinery gene
  MWM05_RS10625 (TbrSNM41_20960) pilW 1964785..1965729 (+) 945 WP_244362606.1 competence protein Machinery gene
  MWM05_RS10630 (TbrSNM41_20970) pilQ 1965741..1968017 (+) 2277 WP_244362608.1 secretin N-terminal domain-containing protein Machinery gene

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 41519.83 Da        Isoelectric Point: 4.4814

>NTDB_id=92940 MWM05_RS10610 WP_244362604.1 1962450..1963583(+) (pilM) [Thermus brockianus strain SNM4-1]
MFSGLSKLFRPRVEALGLEVGASSLKLVELSGHPPTLRAYATRSIPPGTVVDGVVREPGTLAQEIRELLAEARTKKRYVV
SAVPNPAVILRTLQVPKMPPKEMEEAVRWEAERYIPFPIDEVVLDFAPLDPLAEVAEGEQMEVMVAAARQEAVASLIEAL
REAGLTPVVLDVKPFAGLYPLEEELNREPDRTVVAVEIGAESTSLVLARGDRPLAVRLLTLSGKDFTEAIAKSFGLDFLT
AEDVKRTYGLATIPTEDEELLLDFDAERERYSPARIYDAIRPVLVELTQEIRRSLEFFRVQLGDIQPEVGYLYGGGSRLR
GLSTLLTDTLGVNFLVPDPWNGVQIDPKRFDLEKLREAGAELMVPVGLALRGVSPLD

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=92940 MWM05_RS10610 WP_244362604.1 1962450..1963583(+) (pilM) [Thermus brockianus strain SNM4-1]
GTGTTCTCGGGTTTGAGCAAGTTATTTAGACCTCGGGTGGAAGCGTTGGGCCTCGAGGTGGGGGCCTCCAGCCTCAAGCT
GGTGGAGCTTTCCGGCCACCCCCCAACGCTCAGGGCCTACGCCACCCGCTCCATTCCTCCTGGCACCGTGGTGGACGGCG
TGGTCCGGGAACCCGGTACTTTGGCCCAGGAAATCCGCGAGCTCCTGGCCGAAGCCCGGACCAAGAAGCGGTACGTGGTG
AGCGCCGTGCCCAACCCCGCCGTCATCCTGCGCACCCTCCAGGTGCCCAAGATGCCTCCCAAGGAAATGGAGGAAGCTGT
GCGATGGGAGGCGGAGCGCTACATCCCCTTCCCCATTGACGAGGTCGTCCTGGACTTCGCTCCCCTGGACCCTTTGGCGG
AGGTGGCCGAGGGGGAACAGATGGAGGTCATGGTGGCGGCGGCACGCCAGGAAGCCGTGGCCAGCCTCATAGAGGCCCTG
CGGGAAGCGGGGCTCACCCCCGTGGTCCTGGATGTCAAACCCTTCGCCGGGCTTTACCCTTTGGAGGAAGAGCTGAACCG
GGAGCCCGACCGCACCGTGGTGGCGGTGGAAATCGGGGCGGAAAGCACGAGCCTCGTCCTCGCCCGGGGGGACCGCCCCT
TGGCGGTGCGCCTCCTCACCCTTTCGGGCAAGGACTTCACCGAGGCCATCGCCAAGAGCTTCGGCCTGGACTTCCTCACC
GCCGAGGACGTCAAGCGCACCTACGGCCTCGCCACCATCCCCACCGAGGACGAGGAACTCCTCTTGGACTTCGACGCCGA
AAGGGAGCGGTATAGCCCCGCCCGCATCTACGACGCTATCCGGCCCGTTCTGGTGGAGCTGACCCAGGAAATCCGCCGCA
GTTTGGAGTTCTTCCGGGTGCAACTCGGGGATATCCAGCCCGAGGTGGGTTATCTCTATGGCGGGGGAAGCCGGCTACGG
GGGCTTAGCACCTTGCTCACCGACACCCTAGGGGTCAACTTCCTGGTACCCGACCCTTGGAACGGGGTCCAGATAGACCC
CAAGCGCTTTGACCTGGAGAAGCTACGGGAAGCGGGGGCCGAGCTCATGGTGCCCGTGGGCCTGGCGCTGAGGGGGGTGA
GTCCCCTTGATTAG

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(17-372)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Thermus thermophilus HB27

75.597

100

0.756

Multiple sequence alignment