Detailed information    

experimental Experimentally validated

Overview

Name   Cj0683   Type   Machinery gene
Locus tag   Cj0683 Genome accession   NC_002163
Coordinates   636175..636612 (+) Length   145 a.a.
NCBI ID   YP_002344101.1    Uniprot ID   Q0PAJ6
Organism   Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819     
Function   initialization of DNA uptake   
DNA binding and uptake

Function

A mutant lacking the unique periplasmic protein Cj0683 displayed rare but fully functional DNA uptake events. Cj0683 was essential for the efficient initialization of DNA uptake, consistent with the putative function as a competence pilus protein.


Genomic Context

Location: 631175..641612
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Cj0680c uvrB 633589..635562 (-) 1974 YP_002344098.1 excinuclease ABC subunit B -
  Cj0681 - 635716..635946 (+) 231 YP_002344099.1 hypothetical protein -
  Cj0682 - 635936..636178 (+) 243 YP_002344100.1 hypothetical protein -
  Cj0683 Cj0683 636175..636612 (+) 438 YP_002344101.1 hypothetical protein Machinery gene
  Cj0684 priA 636594..638447 (+) 1854 YP_002344102.1 primosome assembly protein PriA -
  Cj0685c cipA 638540..639892 (-) 1353 YP_002344103.1 invasion protein CipA -
  Cj0686 ispG 639998..641071 (+) 1074 YP_002344104.1 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16765.76 Da        Isoelectric Point: 8.0993

>NTDB_id=1244 Cj0683 YP_002344101.1 636175..636612(+) (Cj0683) [Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819]
MNKAFTLLELVFVILILGILSSLSLSFINTTKDEVKILKLKMDYEMLSSALALMRSQMRLKNLNFPEILDNAQNNQAKEK
LFYCLNDCDYSLLDTPIYSDFKSWIKIGKNHYRFALNAKEMVEFIYDSKEGLLKCIGSSRCKDLI

Nucleotide


Download         Length: 438 bp        

>NTDB_id=1244 Cj0683 YP_002344101.1 636175..636612(+) (Cj0683) [Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819]
ATGAATAAAGCTTTTACTCTGCTTGAGCTTGTTTTTGTGATTTTGATTTTGGGAATTTTATCAAGCTTATCTTTATCTTT
TATCAATACTACAAAAGATGAGGTTAAAATTTTAAAATTAAAAATGGACTATGAAATGCTAAGCTCAGCTCTTGCTTTAA
TGCGTAGTCAAATGAGACTTAAAAATTTAAATTTTCCAGAAATTTTAGATAATGCACAAAACAATCAAGCCAAAGAAAAA
CTCTTTTATTGTTTAAATGATTGTGATTATTCTTTGCTTGATACACCTATTTATTCTGATTTTAAATCTTGGATAAAAAT
AGGTAAAAATCACTATAGATTTGCATTAAACGCCAAAGAGATGGTTGAATTTATTTATGATTCTAAAGAAGGTTTGTTAA
AGTGTATAGGAAGTTCTAGATGCAAAGATCTGATATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(2-23)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q0PAJ6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Julia C Golz et al. (2023) Cj0683 Is a Competence Protein Essential for Efficient Initialization of DNA Uptake in Campylobacter jejuni. Biomolecules 13(3):514. [PMID: 36979449]