Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   V1209_RS04765 Genome accession   NZ_CP143255
Coordinates   1066728..1067174 (-) Length   148 a.a.
NCBI ID   WP_009874618.1    Uniprot ID   A3N0P0
Organism   Actinobacillus pleuropneumoniae strain ZJNH2023     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1061728..1072174
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V1209_RS04740 (V1209_04735) yacG 1062635..1062823 (-) 189 WP_005597388.1 DNA gyrase inhibitor YacG -
  V1209_RS04745 (V1209_04740) coaE 1062805..1063443 (-) 639 WP_009874616.1 dephospho-CoA kinase -
  V1209_RS04750 (V1209_04745) - 1063465..1064103 (-) 639 WP_009874617.1 prepilin peptidase -
  V1209_RS04755 (V1209_04750) pilC 1064130..1065332 (-) 1203 WP_005601207.1 type II secretion system F family protein Machinery gene
  V1209_RS04760 (V1209_04755) pilB 1065316..1066710 (-) 1395 WP_005615186.1 GspE/PulE family protein Machinery gene
  V1209_RS04765 (V1209_04760) pilA 1066728..1067174 (-) 447 WP_009874618.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  V1209_RS04770 (V1209_04765) radA 1067356..1068735 (+) 1380 WP_005601211.1 DNA repair protein RadA Machinery gene
  V1209_RS04775 (V1209_04770) - 1068779..1069012 (-) 234 WP_005597399.1 YgjV family protein -
  V1209_RS04780 (V1209_04775) folA 1069139..1069627 (-) 489 WP_005597400.1 type 3 dihydrofolate reductase -
  V1209_RS04785 (V1209_04780) proB 1069714..1070805 (+) 1092 WP_005601213.1 glutamate 5-kinase -
  V1209_RS04790 (V1209_04785) - 1070818..1071594 (+) 777 WP_005597402.1 TSUP family transporter -

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 16006.46 Da        Isoelectric Point: 7.6257

>NTDB_id=928282 V1209_RS04765 WP_009874618.1 1066728..1067174(-) (pilA) [Actinobacillus pleuropneumoniae strain ZJNH2023]
MQKLSLIRPLTNAFTLIELMIVIAIIAILATVAIPSYNSYTQKAALSELLAASASYKTDVEICIYNTGDSKNCSGGQNGV
RKMTELRQAKYLNAITVEGGTITVTGKGNLQEYGYTMTPIHNGSTISWETKCKGEDLSLFPANFCAIN

Nucleotide


Download         Length: 447 bp        

>NTDB_id=928282 V1209_RS04765 WP_009874618.1 1066728..1067174(-) (pilA) [Actinobacillus pleuropneumoniae strain ZJNH2023]
ATGCAAAAACTAAGTCTTATTCGACCGCTTACTAACGCGTTTACTTTAATTGAATTGATGATCGTGATTGCGATTATTGC
CATTTTAGCTACGGTTGCAATTCCGTCATATAACAGTTATACCCAAAAAGCGGCGCTTTCGGAGCTATTGGCGGCATCGG
CTTCTTATAAAACGGATGTCGAGATCTGCATATATAACACCGGAGATTCTAAAAACTGTAGCGGCGGTCAAAACGGTGTC
AGAAAAATGACGGAGCTTAGACAGGCTAAATATTTAAATGCCATTACGGTGGAAGGCGGAACGATTACGGTAACGGGGAA
GGGGAATCTGCAGGAATACGGTTATACGATGACACCGATTCATAACGGTAGCACTATTTCTTGGGAAACGAAATGTAAAG
GGGAGGACTTAAGTTTATTTCCGGCAAATTTCTGTGCGATAAATTAG

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(13-33)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A3N0P0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

68.919

100

0.689
  pilA Haemophilus influenzae 86-028NP

55.172

97.973

0.541
  pilA Haemophilus influenzae Rd KW20

53.103

97.973

0.52