Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   vsple_RS12890 Genome accession   NZ_AP025503
Coordinates   2893305..2893898 (-) Length   197 a.a.
NCBI ID   WP_261882184.1    Uniprot ID   -
Organism   Vibrio pelagius strain ATCC 25916     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2888305..2898898
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  vsple_RS12870 aroB 2889237..2890325 (-) 1089 WP_261882182.1 3-dehydroquinate synthase -
  vsple_RS12875 aroK 2890350..2890868 (-) 519 WP_032550005.1 shikimate kinase AroK -
  vsple_RS12880 - 2891043..2892734 (-) 1692 Protein_2451 type IV pilus secretin PilQ -
  vsple_RS12885 pilP 2892800..2893318 (-) 519 WP_261882183.1 pilus assembly protein PilP Machinery gene
  vsple_RS12890 pilO 2893305..2893898 (-) 594 WP_261882184.1 type 4a pilus biogenesis protein PilO Machinery gene
  vsple_RS12895 pilN 2893891..2894490 (-) 600 WP_261882185.1 PilN domain-containing protein Machinery gene
  vsple_RS12900 pilM 2894474..2895499 (-) 1026 WP_261882186.1 type IV pilus assembly protein PilM Machinery gene
  vsple_RS12905 - 2895647..2898205 (+) 2559 WP_261882187.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 22895.20 Da        Isoelectric Point: 4.5763

>NTDB_id=92240 vsple_RS12890 WP_261882184.1 2893305..2893898(-) (pilO) [Vibrio pelagius strain ATCC 25916]
MVNFQDLDVDEITEWPLLPQLLVVALLMLIVQGFGTWFYVLPLNDELDQMKQQEQTLKTTLRIKANKVATLPALQNQLDE
LTSRYDYLLEQLPVQKELASMLASVNELGLDNHLTFTRIDWGEKQNKAFLYRLPLNIELTGDYHDIGEFSAAIAKLPRII
SFDDVTWQRVSQESSTLHFRVRAYTYQFKAEVKDESR

Nucleotide


Download         Length: 594 bp        

>NTDB_id=92240 vsple_RS12890 WP_261882184.1 2893305..2893898(-) (pilO) [Vibrio pelagius strain ATCC 25916]
ATGGTTAATTTTCAAGATCTCGATGTTGATGAAATTACGGAATGGCCGTTGCTGCCTCAGTTGTTGGTGGTTGCTTTACT
GATGTTGATCGTTCAAGGCTTTGGGACTTGGTTTTATGTGCTGCCACTCAATGATGAGTTGGATCAGATGAAGCAGCAAG
AGCAGACTTTAAAGACCACCCTTCGTATTAAAGCCAATAAAGTGGCGACTTTGCCCGCGCTTCAGAATCAACTCGATGAA
TTGACCAGTCGCTACGACTACTTGCTTGAACAACTGCCAGTTCAAAAAGAGTTGGCGAGCATGCTGGCCTCTGTGAATGA
GCTCGGTTTAGACAATCATCTCACTTTTACTCGCATTGACTGGGGTGAAAAACAGAACAAGGCGTTTCTGTATCGATTGC
CTTTGAATATTGAACTCACGGGTGACTACCACGATATCGGTGAATTTTCTGCGGCGATTGCCAAGTTGCCTCGCATCATT
AGCTTTGATGACGTGACTTGGCAAAGAGTCAGTCAAGAGAGTAGTACTCTGCATTTTCGTGTTCGCGCATACACCTATCA
ATTTAAGGCGGAGGTGAAAGATGAGTCTCGTTAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(47-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio cholerae strain A1552

74.359

98.985

0.736
  pilO Vibrio campbellii strain DS40M4

71.958

95.939

0.69

Multiple sequence alignment