Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   vsple_RS12900 Genome accession   NZ_AP025503
Coordinates   2894474..2895499 (-) Length   341 a.a.
NCBI ID   WP_261882186.1    Uniprot ID   -
Organism   Vibrio pelagius strain ATCC 25916     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2889474..2900499
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  vsple_RS12875 aroK 2890350..2890868 (-) 519 WP_032550005.1 shikimate kinase AroK -
  vsple_RS12880 - 2891043..2892734 (-) 1692 Protein_2451 type IV pilus secretin PilQ -
  vsple_RS12885 pilP 2892800..2893318 (-) 519 WP_261882183.1 pilus assembly protein PilP Machinery gene
  vsple_RS12890 pilO 2893305..2893898 (-) 594 WP_261882184.1 type 4a pilus biogenesis protein PilO Machinery gene
  vsple_RS12895 pilN 2893891..2894490 (-) 600 WP_261882185.1 PilN domain-containing protein Machinery gene
  vsple_RS12900 pilM 2894474..2895499 (-) 1026 WP_261882186.1 type IV pilus assembly protein PilM Machinery gene
  vsple_RS12905 - 2895647..2898205 (+) 2559 WP_261882187.1 penicillin-binding protein 1A -
  vsple_RS12910 oxyR 2898311..2899198 (-) 888 WP_255229749.1 DNA-binding transcriptional regulator OxyR -
  vsple_RS12915 - 2899403..2900131 (+) 729 WP_255229748.1 glutathione peroxidase -

Sequence

Protein


Download         Length: 341 a.a.        Molecular weight: 38167.56 Da        Isoelectric Point: 7.2625

>NTDB_id=92242 vsple_RS12900 WP_261882186.1 2894474..2895499(-) (pilM) [Vibrio pelagius strain ATCC 25916]
MGSSLVTGIDINNHSIKAVVLKPVGELYALVGYKELPITDDIFTDNHTLEYQKTVKKLKELRRGLPFGCRHAAISVPDNT
VISKVLQIESDLEDREKEFSIYQTFAHQSPFPIEELSLDFIKLDDERFGKGLTTSYQVYATRREVVESRSLALSKAGFQP
IVVDTQAHGLLNIWQLATRLYPQKQNWMLVDIGVTQTSLGLISQSGVPFFKDIAFGTQTVNQSDSRSELTISFGSVEQNQ
TFTISLIEKLKRQLQLYASINSMHSLKGIWLTGEGAETPLLAEHLERHFQIPCESLNPLALFENRASNKRYKSMDRHAFG
IAAGIAVSGVKWQGVKHVASN

Nucleotide


Download         Length: 1026 bp        

>NTDB_id=92242 vsple_RS12900 WP_261882186.1 2894474..2895499(-) (pilM) [Vibrio pelagius strain ATCC 25916]
ATGGGTTCGTCATTAGTTACAGGTATAGATATAAACAATCACAGCATAAAAGCGGTGGTTTTAAAGCCCGTTGGAGAGTT
ATATGCCTTAGTGGGGTACAAAGAACTGCCTATTACGGACGACATTTTCACAGATAACCACACCTTGGAGTATCAGAAAA
CTGTAAAGAAACTCAAAGAACTTAGGAGAGGCTTACCATTTGGCTGTCGTCATGCCGCTATCTCTGTCCCAGATAATACC
GTGATTAGCAAAGTCTTGCAGATAGAAAGTGACCTAGAAGACCGAGAAAAAGAGTTTTCTATCTACCAAACTTTTGCCCA
CCAATCACCCTTTCCGATTGAAGAGCTGAGCTTGGATTTCATAAAACTGGACGATGAGCGATTTGGTAAAGGCTTAACCA
CTAGCTATCAAGTCTACGCGACGCGTAGAGAGGTAGTGGAGAGTCGCTCTTTAGCCTTATCTAAGGCAGGGTTTCAACCC
ATCGTTGTCGATACCCAAGCTCATGGTTTACTTAACATTTGGCAGTTGGCTACTCGACTTTATCCTCAGAAGCAGAACTG
GATGTTGGTCGATATAGGGGTGACGCAAACATCATTAGGGCTGATTTCTCAAAGCGGAGTGCCTTTTTTTAAAGATATCG
CCTTCGGTACGCAAACCGTCAATCAAAGTGACTCTCGTAGTGAGTTGACGATTAGCTTTGGCTCAGTTGAACAGAATCAA
ACTTTTACGATCAGTTTAATTGAGAAACTCAAACGGCAGTTGCAGCTTTATGCCTCAATCAATTCAATGCACTCACTGAA
AGGCATTTGGTTGACAGGTGAGGGGGCGGAAACTCCGTTATTGGCTGAGCATTTGGAGCGACATTTTCAGATCCCTTGTG
AGTCACTCAACCCTTTGGCGCTATTTGAAAATAGAGCCTCTAATAAACGCTATAAGAGCATGGACCGTCACGCTTTTGGC
ATCGCGGCTGGTATTGCAGTCAGTGGGGTGAAGTGGCAAGGAGTGAAACATGTTGCATCAAATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-327)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

51.327

99.413

0.51
  pilM Vibrio campbellii strain DS40M4

46.628

100

0.466

Multiple sequence alignment