Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   VDS57_RS00990 Genome accession   NZ_CP141839
Coordinates   176797..177414 (-) Length   205 a.a.
NCBI ID   WP_046160608.1    Uniprot ID   -
Organism   Bacillus subtilis strain PN1236     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 171797..182414
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VDS57_RS00960 (VDS57_00960) rpsT 172099..172365 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  VDS57_RS00965 (VDS57_00965) holA 172380..173423 (-) 1044 WP_029317888.1 DNA polymerase III subunit delta -
  VDS57_RS00970 (VDS57_00970) - 173463..173612 (-) 150 WP_003229985.1 hypothetical protein -
  VDS57_RS00975 (VDS57_00975) yqzM 173653..173787 (+) 135 WP_003229983.1 YqzM family protein -
  VDS57_RS00980 (VDS57_00980) comEC 173827..176157 (-) 2331 WP_069837640.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  VDS57_RS00985 (VDS57_00985) comEB 176161..176730 (-) 570 WP_003229978.1 ComE operon protein 2 -
  VDS57_RS00990 (VDS57_00990) comEA 176797..177414 (-) 618 WP_046160608.1 competence protein ComEA Machinery gene
  VDS57_RS00995 (VDS57_00995) comER 177498..178319 (+) 822 WP_032726195.1 late competence protein ComER -
  VDS57_RS01000 (VDS57_01000) yqeM 178385..179128 (-) 744 WP_069837641.1 class I SAM-dependent DNA methyltransferase -
  VDS57_RS01005 (VDS57_01005) rsfS 179125..179481 (-) 357 WP_014480315.1 ribosome silencing factor -
  VDS57_RS01010 (VDS57_01010) yqeK 179499..180059 (-) 561 WP_014480316.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  VDS57_RS01015 (VDS57_01015) nadD 180049..180618 (-) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  VDS57_RS01020 (VDS57_01020) yhbY 180630..180920 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  VDS57_RS01025 (VDS57_01025) aroE 180914..181756 (-) 843 WP_014480317.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21738.48 Da        Isoelectric Point: 4.7284

>NTDB_id=920121 VDS57_RS00990 WP_046160608.1 176797..177414(-) (comEA) [Bacillus subtilis strain PN1236]
MNWLNQHKKAIVLAASAAVFTAIMIFLATGKNKEPVKQAVPTEAENTVVKQEANNDESSETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEEITKVSGIGEKLFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=920121 VDS57_RS00990 WP_046160608.1 176797..177414(-) (comEA) [Bacillus subtilis strain PN1236]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTGTTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGGCAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAGCGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGAGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTTTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGAAATCACTAAGGT
TTCAGGAATAGGTGAAAAGTTATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(63-118)

Helix-hairpin-helix motif (HHH_3)

(142-203)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

97.561

100

0.976
  comEA Staphylococcus aureus MW2

35.909

100

0.385
  comEA Staphylococcus aureus N315

35

100

0.376