Detailed information NTG0919053

Bioinformatically predicted

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: VNN51_RS10150
• Genome accession: NZ_CP141727
• Coordinates: 2044038..2045294 (+)
• Length: 418 a.a.
• NCBI ID: WP_213496707.1
• Uniprot ID: -
• Organism: Lactococcus formosensis strain M20011502 FF20a2
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2039038..2050294

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
VNN51_RS10125 (VNN51_10130)	glyQ	2039188..2040156 (-)	969	WP_017369700.1	glycine--tRNA ligase subunit alpha	-
VNN51_RS10130 (VNN51_10135)	-	2040170..2040949 (-)	780	WP_213432715.1	formate/nitrite transporter family protein	-
VNN51_RS10135 (VNN51_10140)	-	2041267..2042288 (+)	1022	Protein_1949	alpha/beta hydrolase	-
VNN51_RS10140 (VNN51_10145)	-	2042325..2043347 (-)	1023	WP_017369703.1	DUF475 domain-containing protein	-
VNN51_RS10145 (VNN51_10150)	-	2043360..2043983 (-)	624	WP_213496705.1	YigZ family protein	-
VNN51_RS10150 (VNN51_10155)	comFA	2044038..2045294 (+)	1257	WP_213496707.1	DEAD/DEAH box helicase	Machinery gene
VNN51_RS10155 (VNN51_10160)	-	2045291..2045935 (+)	645	WP_346349575.1	ComF family protein	-
VNN51_RS10160 (VNN51_10165)	-	2045938..2046399 (-)	462	WP_017369707.1	diacylglycerol kinase family protein	-
VNN51_RS10165 (VNN51_10170)	ybeY	2046383..2046871 (-)	489	WP_014024619.1	rRNA maturation RNase YbeY	-
VNN51_RS10170 (VNN51_10175)	-	2046881..2047354 (-)	474	WP_213432720.1	NUDIX hydrolase	-
VNN51_RS10175 (VNN51_10180)	-	2047356..2048315 (-)	960	WP_004257953.1	PhoH family protein	-
VNN51_RS10180 (VNN51_10185)	ald	2048434..2049540 (-)	1107	WP_213496711.1	alanine dehydrogenase	-

Sequence

Protein

• Length: 418 a.a.
• Molecular weight: 47888.97 Da
• Isoelectric Point: 10.1056

>NTDB_id=919053 VNN51_RS10150 WP_213496707.1 2044038..2045294(+) (comFA) [Lactococcus formosensis strain M20011502 FF20a2]
MNELYGRLLLQKELTKIPDEVILFDGMKEVSKTIIMCNRCGKKSKKKEVYLPVGAYYCPHCIQMGRVRSDEKLYHLPQKN
FTAVSCLKWQGKLTGPQQYLSDNLLNLHRQQKTVLVQAVTGAGKTEMIYQVIDATLKKGKAVGLTSPRIDVCLELYYRLK
RDFSCPISLLHGKGEKYSRSPLVIATTHQLLRFRHAFDLLILDEVDAFPFPDNEMLYFALAQARKPSSSLIYLTATTTNN
LEKQVKLGQIEKLQLPRRFHGFPLVLPQFFWQSKFYKMIKKQRESGLPLLIFAPEIRLGEKLCQDLQSSFPHEEIAFVAS
TSLERLESVERFRHGKVSILVSTTILERGVTFPKVDVFVFQSHHHNFTRSSLIQIAGRVGRSTERPDGKVIFFHLGKTTA
MLEAYKDIRNMNKAGGFQ

Nucleotide

• Length: 1257 bp

>NTDB_id=919053 VNN51_RS10150 WP_213496707.1 2044038..2045294(+) (comFA) [Lactococcus formosensis strain M20011502 FF20a2]
ATGAATGAATTATACGGTAGACTTCTTCTGCAAAAAGAATTGACGAAAATACCAGACGAAGTCATACTTTTTGATGGTAT
GAAAGAAGTTTCAAAAACGATTATCATGTGTAACCGCTGTGGTAAAAAGAGTAAGAAAAAAGAGGTTTATCTCCCCGTTG
GTGCTTATTATTGCCCGCATTGTATTCAGATGGGGCGTGTTCGCTCTGATGAAAAACTTTATCACCTTCCACAGAAAAAT
TTTACTGCAGTTTCTTGTCTAAAATGGCAAGGAAAGCTCACAGGTCCTCAGCAATATCTCTCCGATAATTTACTTAATCT
GCATCGGCAGCAAAAAACAGTGCTGGTACAGGCTGTAACAGGGGCAGGAAAGACGGAAATGATTTATCAAGTCATTGATG
CCACCTTAAAGAAAGGAAAAGCTGTGGGTTTGACGAGTCCACGCATTGATGTTTGTCTAGAACTTTACTATCGCTTAAAG
AGGGATTTTTCCTGTCCCATCTCGCTTTTGCATGGAAAGGGTGAGAAATACAGCCGTTCTCCTCTTGTAATTGCAACCAC
ACATCAGCTTTTACGTTTTCGTCATGCCTTTGATTTACTCATTCTCGACGAAGTTGACGCTTTTCCATTTCCAGATAATG
AAATGCTTTATTTTGCGCTGGCTCAAGCTCGAAAACCTTCTTCAAGCTTGATTTATCTTACTGCAACAACCACCAACAAT
TTGGAAAAACAAGTCAAGTTGGGGCAAATCGAAAAGTTACAACTTCCCCGACGCTTTCATGGTTTTCCACTAGTACTTCC
GCAATTTTTTTGGCAGAGCAAGTTTTACAAGATGATCAAAAAACAACGCGAATCTGGTTTACCATTACTTATCTTTGCCC
CTGAAATAAGGCTGGGAGAAAAACTGTGCCAAGACTTACAAAGCAGTTTTCCTCATGAAGAAATTGCCTTTGTTGCATCA
ACGAGTCTTGAACGCTTAGAGTCTGTGGAACGTTTTCGACACGGAAAAGTTTCTATCCTTGTCTCAACGACTATTTTAGA
ACGTGGCGTAACCTTCCCTAAGGTTGACGTTTTTGTATTTCAAAGTCACCACCATAATTTCACAAGATCAAGCCTCATCC
AAATTGCCGGACGAGTAGGTCGAAGTACCGAAAGACCTGACGGCAAAGTGATTTTCTTTCATTTAGGAAAAACAACAGCA
ATGTTGGAAGCTTATAAAGATATCAGAAATATGAATAAAGCAGGAGGTTTCCAATGA

Domains

Predicted by InterproScan.

• Helicase conserved C-terminal domain (Helicase_C) (274-382)

• Type III restriction enzyme, res subunit (ResIII) (93-236)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Lactococcus lactis subsp. cremoris KW2	56.642	95.455	0.541
comFA/cflA	Streptococcus pneumoniae TIGR4	52.927	100	0.541
comFA/cflA	Streptococcus pneumoniae Rx1	52.927	100	0.541
comFA/cflA	Streptococcus pneumoniae D39	52.927	100	0.541
comFA/cflA	Streptococcus pneumoniae R6	52.927	100	0.541
comFA/cflA	Streptococcus mitis SK321	52.225	100	0.533
comFA/cflA	Streptococcus mitis NCTC 12261	51.522	100	0.526
comFA	Bacillus subtilis subsp. subtilis str. 168	38.041	100	0.4
comFA	Latilactobacillus sakei subsp. sakei 23K	38.035	94.976	0.361