Detailed information NTG0918916

Bioinformatically predicted

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: VNN39_RS09370
• Genome accession: NZ_CP141721
• Coordinates: 1949776..1951032 (+)
• Length: 418 a.a.
• NCBI ID: WP_338997610.1
• Uniprot ID: -
• Organism: Lactococcus formosensis strain M04020401 EE5e5
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1944776..1956032

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
VNN39_RS09345 (VNN39_09345)	glyQ	1944925..1945893 (-)	969	WP_339007091.1	glycine--tRNA ligase subunit alpha	-
VNN39_RS09350 (VNN39_09350)	-	1945907..1946686 (-)	780	WP_338997607.1	formate/nitrite transporter family protein	-
VNN39_RS09355 (VNN39_09355)	-	1947004..1948026 (+)	1023	WP_338997609.1	alpha/beta hydrolase	-
VNN39_RS09360 (VNN39_09360)	-	1948063..1949085 (-)	1023	WP_019293557.1	DUF475 domain-containing protein	-
VNN39_RS09365 (VNN39_09365)	-	1949098..1949721 (-)	624	WP_017369704.1	YigZ family protein	-
VNN39_RS09370 (VNN39_09370)	comFA	1949776..1951032 (+)	1257	WP_338997610.1	DEAD/DEAH box helicase	Machinery gene
VNN39_RS09375 (VNN39_09375)	-	1951029..1951673 (+)	645	WP_338997611.1	ComF family protein	-
VNN39_RS09380 (VNN39_09380)	-	1951676..1952137 (-)	462	WP_096368379.1	diacylglycerol kinase family protein	-
VNN39_RS09385 (VNN39_09385)	ybeY	1952121..1952609 (-)	489	WP_338997612.1	rRNA maturation RNase YbeY	-
VNN39_RS09390 (VNN39_09390)	-	1952619..1953092 (-)	474	WP_017369708.1	NUDIX hydrolase	-
VNN39_RS09395 (VNN39_09395)	-	1953094..1954053 (-)	960	WP_096368380.1	PhoH family protein	-
VNN39_RS09400 (VNN39_09400)	ald	1954172..1955278 (-)	1107	WP_338997613.1	alanine dehydrogenase	-
VNN39_RS09405 (VNN39_09405)	-	1955588..1955929 (+)	342	WP_243415387.1	AAA family ATPase	-

Sequence

Protein

• Length: 418 a.a.
• Molecular weight: 47844.92 Da
• Isoelectric Point: 10.1056

>NTDB_id=918916 VNN39_RS09370 WP_338997610.1 1949776..1951032(+) (comFA) [Lactococcus formosensis strain M04020401 EE5e5]
MNELYGRLLLQKELTKIPDEVILFDGMKEVSKTIVMCNRCGKKSKKKEVYLPVGAYYCPHCIQMGRVRSDEKLYHLPQKN
FAAVSCLKWQGKLTGPQQYISDNLLNLHRQQKTVLVQAVTGAGKTEMIYQVIDATLKKGKAVGLTSPRIDVCLELYYRLK
RDFSCPISLLHGKGEKYSRSPLVIATTHQLLRFRHAFDLLILDEVDAFPFPDNEMLYFALAQARKPSSSLIYLTATTTNN
LEKQVKLGQIEKLQLPRRFHGFPLVLPQFFWQSKFYKMIKKQRESGLPLLIFAPEIRLGEKLCQDLQSSFPHEEIAFVAS
TSLERLESVERFRHGKVSILVSTTILERGVTFPKVDVFVFQSHHHNFTRSSLIQIAGRVGRSTERPDGKVIFFHLGKTTA
MLEAYKDIRNMNKAGGFQ

Nucleotide

• Length: 1257 bp

>NTDB_id=918916 VNN39_RS09370 WP_338997610.1 1949776..1951032(+) (comFA) [Lactococcus formosensis strain M04020401 EE5e5]
ATGAATGAATTATACGGTAGACTTCTTCTGCAAAAAGAATTGACGAAAATACCAGACGAAGTCATACTTTTTGATGGTAT
GAAAGAAGTTTCAAAAACGATTGTCATGTGTAACCGCTGTGGTAAAAAGAGTAAGAAAAAAGAGGTTTATCTCCCCGTTG
GTGCTTATTATTGCCCGCATTGTATTCAGATGGGGCGTGTTCGCTCTGATGAAAAACTTTATCACCTTCCACAGAAAAAT
TTTGCTGCAGTTTCTTGTCTAAAATGGCAGGGAAAGCTCACAGGTCCTCAGCAATATATCTCCGATAATTTACTTAATCT
GCATCGGCAGCAAAAAACAGTGCTGGTACAGGCTGTAACAGGGGCAGGAAAGACGGAAATGATTTATCAAGTCATTGATG
CCACCTTAAAGAAAGGAAAAGCTGTGGGTTTGACGAGTCCACGCATTGATGTTTGTCTAGAACTTTACTATCGCTTAAAG
AGGGATTTTTCCTGTCCCATCTCGCTTTTGCATGGAAAGGGTGAGAAATACAGCCGTTCTCCTCTTGTAATTGCAACCAC
ACATCAGCTTTTACGTTTTCGTCATGCCTTTGATTTACTCATTCTCGACGAAGTTGACGCTTTTCCATTTCCAGATAATG
AAATGCTTTATTTTGCGCTGGCTCAAGCTCGAAAACCTTCTTCAAGCTTGATTTATCTTACTGCAACAACCACCAACAAT
TTGGAAAAACAAGTCAAGTTGGGGCAAATCGAAAAGTTACAACTTCCCCGACGCTTTCATGGTTTTCCACTAGTACTTCC
GCAATTTTTTTGGCAGAGCAAGTTTTACAAGATGATCAAAAAACAACGCGAATCTGGTTTACCATTACTTATCTTTGCCC
CTGAAATAAGGCTGGGAGAAAAACTGTGCCAAGACTTACAAAGCAGTTTTCCTCATGAAGAAATTGCCTTTGTTGCATCA
ACGAGTCTTGAACGCTTAGAGTCTGTGGAACGTTTTCGACACGGAAAAGTTTCTATCCTTGTCTCAACGACTATTTTAGA
ACGTGGCGTAACCTTCCCTAAGGTTGACGTTTTTGTATTTCAAAGTCACCACCATAATTTCACAAGATCAAGCCTCATCC
AAATTGCCGGACGAGTAGGTCGAAGTACCGAAAGACCTGACGGCAAAGTGATTTTCTTTCATTTAGGAAAAACAACAGCA
ATGTTGGAAGCTTATAAAGATATCAGAAATATGAATAAAGCAGGAGGTTTCCAATGA

Domains

Predicted by InterproScan.

• Helicase conserved C-terminal domain (Helicase_C) (274-382)

• Type III restriction enzyme, res subunit (ResIII) (93-236)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Lactococcus lactis subsp. cremoris KW2	57.143	95.455	0.545
comFA/cflA	Streptococcus pneumoniae TIGR4	52.927	100	0.541
comFA/cflA	Streptococcus pneumoniae Rx1	52.927	100	0.541
comFA/cflA	Streptococcus pneumoniae D39	52.927	100	0.541
comFA/cflA	Streptococcus pneumoniae R6	52.927	100	0.541
comFA/cflA	Streptococcus mitis SK321	52.459	100	0.536
comFA/cflA	Streptococcus mitis NCTC 12261	51.522	100	0.526
comFA	Bacillus subtilis subsp. subtilis str. 168	38.041	100	0.4
comFA	Latilactobacillus sakei subsp. sakei 23K	38.035	94.976	0.361