Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   OC193_RS14360 Genome accession   NZ_AP025476
Coordinates   3180348..3181373 (-) Length   341 a.a.
NCBI ID   WP_048663002.1    Uniprot ID   -
Organism   Vibrio crassostreae strain LMG 22240     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3175348..3186373
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OC193_RS14335 aroK 3176207..3176725 (-) 519 WP_017061125.1 shikimate kinase AroK -
  OC193_RS14340 pilQ 3176902..3178596 (-) 1695 WP_048662999.1 type IV pilus secretin PilQ Machinery gene
  OC193_RS14345 pilP 3178656..3179174 (-) 519 WP_048663000.1 pilus assembly protein PilP Machinery gene
  OC193_RS14350 pilO 3179161..3179772 (-) 612 WP_048659164.1 type 4a pilus biogenesis protein PilO Machinery gene
  OC193_RS14355 pilN 3179765..3180364 (-) 600 WP_048663001.1 PilN domain-containing protein Machinery gene
  OC193_RS14360 pilM 3180348..3181373 (-) 1026 WP_048663002.1 type IV pilus assembly protein PilM Machinery gene
  OC193_RS14365 - 3181525..3184086 (+) 2562 WP_048663003.1 penicillin-binding protein 1A -
  OC193_RS14370 oxyR 3184198..3185091 (-) 894 WP_048663004.1 DNA-binding transcriptional regulator OxyR -
  OC193_RS14375 - 3185090..3185269 (+) 180 WP_080967396.1 hypothetical protein -
  OC193_RS14380 - 3185314..3186042 (+) 729 WP_048659171.1 glutathione peroxidase -

Sequence

Protein


Download         Length: 341 a.a.        Molecular weight: 38131.52 Da        Isoelectric Point: 6.6638

>NTDB_id=91891 OC193_RS14360 WP_048663002.1 3180348..3181373(-) (pilM) [Vibrio crassostreae strain LMG 22240]
MGSSLITGIDINHHSIKAVVLKPVGELYALVGYKELPISDDIFTANHTLEYQKTVKKLKELRKGLPFGCRNVAISVPDNT
VISKVLQIESELEDREKEFSIYQTFAHQSPFPIEELSLDFVKLEDKRFGKGSTSSYQVYATRKEVVDSRADALTKAGFKP
VVVDTQAHGLLNIWQLASRLYPDKNNWLLVDVGIDQTSLGVIPQNSAPFYKDIAFGTQALRSTDNPSDIEGVFGTAEDTH
QFIVHLIEKLKRQLQLYSSGNAQQPISGIWLMGEGASIPMVTEELERHFQLSCESLNPLSLFDNKVAKRRRLPMDWQHFG
IAAGMAMSGLKWQGEKHVASN

Nucleotide


Download         Length: 1026 bp        

>NTDB_id=91891 OC193_RS14360 WP_048663002.1 3180348..3181373(-) (pilM) [Vibrio crassostreae strain LMG 22240]
ATGGGTTCATCATTAATTACAGGTATAGATATAAATCATCACAGCATCAAAGCCGTGGTACTAAAACCCGTGGGGGAGTT
GTATGCCCTAGTGGGATACAAAGAGCTGCCGATTTCGGACGACATTTTTACAGCTAACCATACTTTGGAGTATCAGAAAA
CTGTTAAGAAACTTAAAGAACTTAGGAAAGGACTGCCTTTTGGTTGTCGCAACGTCGCCATTTCGGTACCTGATAACACT
GTGATCAGCAAAGTACTACAAATAGAGAGTGAGTTAGAAGACAGAGAAAAAGAGTTTTCTATCTATCAAACCTTCGCCCA
TCAATCTCCCTTTCCTATCGAGGAGCTGAGTTTAGATTTTGTAAAGCTGGAAGACAAACGATTTGGTAAAGGTTCGACAA
GCAGTTATCAGGTATACGCCACTCGTAAGGAAGTCGTCGATAGCCGAGCGGATGCATTAACTAAGGCTGGTTTTAAACCT
GTGGTGGTAGATACACAGGCACATGGGCTGCTAAACATCTGGCAATTAGCTTCGCGCTTGTATCCCGATAAGAATAACTG
GCTGCTGGTGGATGTTGGAATCGACCAAACGTCGTTAGGAGTTATCCCGCAGAATTCAGCGCCTTTCTATAAAGACATCG
CTTTTGGTACTCAAGCTCTTCGCAGCACGGATAACCCAAGTGATATAGAAGGTGTGTTCGGCACGGCTGAAGATACCCAT
CAATTCATTGTTCATTTGATTGAGAAGCTCAAGCGCCAATTACAGCTCTATTCATCGGGAAATGCGCAGCAACCTATCTC
TGGGATCTGGCTGATGGGTGAGGGTGCGAGCATCCCAATGGTAACCGAAGAGTTAGAGCGCCATTTTCAGCTGAGCTGTG
AGTCATTGAATCCTTTGTCTCTGTTTGATAACAAGGTCGCCAAGAGACGTCGACTGCCAATGGACTGGCAACACTTTGGT
ATTGCCGCGGGTATGGCGATGAGTGGACTCAAGTGGCAGGGAGAAAAGCATGTTGCATCAAATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-220)

Type IV pilus assembly protein PilM; (PilM_2)

(242-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

54.545

100

0.545
  pilM Vibrio campbellii strain DS40M4

48.538

100

0.487

Multiple sequence alignment