Detailed information NTG0918870

Bioinformatically predicted

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: VNN53_RS09430
• Genome accession: NZ_CP141720
• Coordinates: 1946109..1947365 (+)
• Length: 418 a.a.
• NCBI ID: WP_338961697.1
• Uniprot ID: -
• Organism: Lactococcus garvieae strain CAHFS22OV20.5B5
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1941109..1952365

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
VNN53_RS09405 (VNN53_09405)	glyQ	1941263..1942231 (-)	969	WP_004257927.1	glycine--tRNA ligase subunit alpha	-
VNN53_RS09410 (VNN53_09410)	-	1942245..1943024 (-)	780	WP_338961689.1	formate/nitrite transporter family protein	-
VNN53_RS09415 (VNN53_09415)	-	1943343..1944365 (+)	1023	WP_338961692.1	alpha/beta hydrolase	-
VNN53_RS09420 (VNN53_09420)	-	1944395..1945417 (-)	1023	WP_023889525.1	DUF475 domain-containing protein	-
VNN53_RS09425 (VNN53_09425)	-	1945431..1946054 (-)	624	WP_176490183.1	YigZ family protein	-
VNN53_RS09430 (VNN53_09430)	comFA	1946109..1947365 (+)	1257	WP_338961697.1	DEAD/DEAH box helicase	Machinery gene
VNN53_RS09435 (VNN53_09435)	-	1947362..1948006 (+)	645	WP_338961700.1	ComF family protein	-
VNN53_RS09440 (VNN53_09440)	-	1948009..1948470 (-)	462	WP_074750990.1	diacylglycerol kinase family protein	-
VNN53_RS09445 (VNN53_09445)	ybeY	1948454..1948942 (-)	489	WP_014024619.1	rRNA maturation RNase YbeY	-
VNN53_RS09450 (VNN53_09450)	-	1948952..1949425 (-)	474	WP_338961716.1	NUDIX hydrolase	-
VNN53_RS09455 (VNN53_09455)	-	1949427..1950386 (-)	960	WP_004257953.1	PhoH family protein	-
VNN53_RS09460 (VNN53_09460)	ald	1950504..1951610 (-)	1107	WP_338961718.1	alanine dehydrogenase	-
VNN53_RS09465 (VNN53_09465)	rpmA	1951961..1952245 (-)	285	WP_004257956.1	50S ribosomal protein L27	-

Sequence

Protein

• Length: 418 a.a.
• Molecular weight: 47919.75 Da
• Isoelectric Point: 10.0377

>NTDB_id=918870 VNN53_RS09430 WP_338961697.1 1946109..1947365(+) (comFA) [Lactococcus garvieae strain CAHFS22OV20.5B5]
MDELYGRLLLQKELTKLPDKATLFDGMQDVSKTVMMCNRCGKKIKKKEVLLPVGAYYCPHCIQMGRVRSDEKLYHLPQED
FSAASFLNWKGKLTASQQHVSDSLVNLHQQQKTVLVQAVTGAGKTEMIYQSIDNTLKKGKAVGLTSPRIDVCLELYHRLK
RDFSCPISLLHGKSEKYSRSPLVIATTHQLMRFRHAFDLLILDEVDAFPFPDNEMLYFALAQARKPSSSLIYLTATTTDN
LEKQVKLGQIEKLQLPRRFHGFPLVLPQFFWQSKFYKMVKKQRESGFPLLIFVPEIRQGEKLCQDLQSNFPHEEIAFVAS
TSLERLEAVERFRQGNISILVSTTILERGVTFPKVDVFVFQSHHHNFTRSRLIQIAGRVGRSTERPEGKVFFFHLGKTTA
MLEAYKNIRNMNKAGGFL

Nucleotide

• Length: 1257 bp

>NTDB_id=918870 VNN53_RS09430 WP_338961697.1 1946109..1947365(+) (comFA) [Lactococcus garvieae strain CAHFS22OV20.5B5]
ATGGATGAATTATATGGTAGACTCCTTTTACAAAAAGAATTGACGAAACTTCCAGATAAAGCAACACTTTTTGATGGGAT
GCAAGATGTTTCCAAAACAGTGATGATGTGCAACCGCTGTGGCAAAAAGATTAAGAAAAAAGAGGTCCTTCTCCCTGTTG
GTGCTTATTATTGTCCCCATTGTATTCAAATGGGACGTGTTCGCTCGGATGAAAAACTTTACCACCTTCCACAGGAGGAT
TTTTCAGCAGCTTCTTTTCTCAACTGGAAGGGAAAACTCACAGCATCTCAGCAACATGTATCCGATAGCCTAGTCAATTT
GCATCAGCAACAAAAAACAGTACTGGTACAGGCGGTTACTGGTGCAGGAAAAACCGAAATGATTTATCAGAGCATTGATA
ACACCCTTAAAAAAGGAAAAGCTGTAGGTTTAACTAGCCCGCGTATTGATGTCTGTCTAGAGCTCTATCATCGCTTAAAG
AGAGATTTTTCTTGTCCCATCTCGCTTTTGCACGGAAAGAGTGAAAAATACAGCCGATCGCCACTCGTAATTGCAACGAC
ACATCAGCTTATGCGTTTTCGCCATGCCTTTGACTTACTCATTCTTGATGAGGTGGATGCCTTTCCCTTTCCAGACAATG
AAATGCTTTATTTTGCGCTGGCTCAAGCCCGTAAACCTTCTTCAAGCTTGATTTATCTTACTGCAACCACCACCGATAAT
TTGGAAAAACAAGTCAAATTAGGACAAATCGAGAAGCTACAGCTTCCACGACGTTTTCATGGTTTTCCTCTGGTACTCCC
GCAATTTTTTTGGCAAAGCAAGTTTTACAAAATGGTCAAAAAACAACGCGAGTCTGGTTTTCCCTTACTTATCTTTGTCC
CTGAAATAAGACAGGGAGAAAAACTCTGCCAAGATTTACAGAGTAATTTTCCTCATGAAGAAATTGCTTTTGTAGCTTCA
ACAAGTCTTGAACGCTTAGAGGCTGTGGAACGCTTTCGTCAGGGCAATATTTCTATCCTTGTCTCAACGACCATTTTAGA
ACGTGGTGTAACTTTCCCTAAGGTTGACGTTTTTGTGTTTCAAAGTCACCACCATAATTTCACAAGATCAAGGCTCATCC
AAATTGCCGGACGAGTAGGTCGAAGTACCGAAAGACCTGAGGGTAAGGTATTTTTCTTTCATCTAGGAAAAACAACAGCG
ATGTTGGAAGCCTATAAAAATATCAGAAATATGAATAAAGCAGGGGGGTTCCTATGA

Domains

Predicted by InterproScan.

• Helicase conserved C-terminal domain (Helicase_C) (274-382)

• Type III restriction enzyme, res subunit (ResIII) (92-236)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Lactococcus lactis subsp. cremoris KW2	57.653	93.78	0.541
comFA/cflA	Streptococcus mitis SK321	52.113	100	0.531
comFA/cflA	Streptococcus pneumoniae TIGR4	52.113	100	0.531
comFA/cflA	Streptococcus pneumoniae Rx1	52.113	100	0.531
comFA/cflA	Streptococcus pneumoniae D39	52.113	100	0.531
comFA/cflA	Streptococcus pneumoniae R6	52.113	100	0.531
comFA/cflA	Streptococcus mitis NCTC 12261	51.174	100	0.522
comFA	Bacillus subtilis subsp. subtilis str. 168	38.614	96.651	0.373
comFA	Latilactobacillus sakei subsp. sakei 23K	38.539	94.976	0.366