Detailed information NTG0918603

Bioinformatically predicted

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: VNN32_RS10220
• Genome accession: NZ_CP141703
• Coordinates: 1986527..1987783 (+)
• Length: 418 a.a.
• NCBI ID: WP_046401091.1
• Uniprot ID: -
• Organism: Lactococcus petauri strain R21-74 AL-1
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1981527..1992783

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
VNN32_RS10195 (VNN32_10190)	glyQ	1981678..1982646 (-)	969	WP_019292765.1	glycine--tRNA ligase subunit alpha	-
VNN32_RS10200 (VNN32_10195)	-	1982660..1983439 (-)	780	WP_019292766.1	formate/nitrite transporter family protein	-
VNN32_RS10205 (VNN32_10200)	-	1983757..1984779 (+)	1023	WP_338975854.1	alpha/beta hydrolase	-
VNN32_RS10210 (VNN32_10205)	-	1984814..1985836 (-)	1023	WP_019293557.1	DUF475 domain-containing protein	-
VNN32_RS10215 (VNN32_10210)	-	1985849..1986472 (-)	624	WP_019292769.1	YigZ family protein	-
VNN32_RS10220 (VNN32_10215)	comFA	1986527..1987783 (+)	1257	WP_046401091.1	DEAD/DEAH box helicase	Machinery gene
VNN32_RS10225 (VNN32_10220)	-	1987780..1988424 (+)	645	WP_019292771.1	ComF family protein	-
VNN32_RS10230 (VNN32_10225)	-	1988427..1988888 (-)	462	WP_019292772.1	diacylglycerol kinase family protein	-
VNN32_RS10235 (VNN32_10230)	ybeY	1988872..1989360 (-)	489	WP_019292773.1	rRNA maturation RNase YbeY	-
VNN32_RS10240 (VNN32_10235)	-	1989370..1989843 (-)	474	WP_019292774.1	NUDIX hydrolase	-
VNN32_RS10245 (VNN32_10240)	-	1989845..1990804 (-)	960	WP_019292775.1	PhoH family protein	-
VNN32_RS10250 (VNN32_10245)	ald	1990924..1992030 (-)	1107	WP_046401092.1	alanine dehydrogenase	-

Sequence

Protein

• Length: 418 a.a.
• Molecular weight: 48009.94 Da
• Isoelectric Point: 10.1835

>NTDB_id=918603 VNN32_RS10220 WP_046401091.1 1986527..1987783(+) (comFA) [Lactococcus petauri strain R21-74 AL-1]
MNELYGRLLLQKELSKIPDKATLFDSMHDISRTVIMCNRCGKKSKKKEVLLPVGAYYCPHCIQMGRVRSDEKLYHLPQED
FLAASFLNWQGKLTAPQQYISDTLVKLHQQQKTVLVQAVTGAGKTEMIYQMIDNTLKKGKAVALTSPRIDVCLELYYRLK
RDFSCPISLLHGKSEKYSRSPLVIATTHQLMRFRHAFDLLILDEVDAFPFPDNDMLSFALTQARKPSSSLIYLTATTTDN
LERQVKLGQIEKLQLPRRFHGFPLVLPQFFWQSKFYKMVKKQRESGFPLLIFVPEIRLGEKLNQDLQSSFPHEKIAFVAS
TSLERLEAVERFRQGNISILVSTTILERGVTFPKVDVFVFQSHHHNFTRSSLIQIAGRVGRSTERPDGKVFFFHLGKITA
MLEAYKNIRNMNKAGGFQ

Nucleotide

• Length: 1257 bp

>NTDB_id=918603 VNN32_RS10220 WP_046401091.1 1986527..1987783(+) (comFA) [Lactococcus petauri strain R21-74 AL-1]
ATGAATGAATTATATGGTAGACTCCTTTTACAAAAAGAATTGTCGAAAATCCCAGACAAAGCAACACTTTTTGATAGTAT
GCACGATATTTCAAGAACAGTGATTATGTGCAATCGCTGTGGCAAAAAAAGTAAGAAAAAAGAGGTCCTACTCCCCGTTG
GTGCTTATTATTGTCCCCATTGTATTCAGATGGGACGTGTTCGCTCGGATGAAAAACTTTATCATCTTCCTCAGGAGGAT
TTTTTAGCAGCTTCTTTTCTAAACTGGCAGGGAAAACTCACAGCCCCTCAGCAATATATATCTGATACTCTAGTCAAATT
ACATCAGCAACAAAAAACGGTGCTGGTACAGGCTGTCACGGGTGCAGGAAAAACTGAAATGATTTATCAGATGATTGATA
ACACCCTTAAAAAAGGAAAAGCTGTAGCTTTAACTAGCCCTCGTATTGATGTCTGTCTAGAACTCTACTATCGCTTAAAG
CGAGATTTTTCTTGTCCCATCTCGCTTTTACACGGAAAAAGTGAGAAATACAGCCGCTCGCCACTCGTAATTGCGACGAC
ACATCAGCTTATGCGTTTTCGTCATGCCTTTGACTTACTCATTCTTGATGAGGTTGATGCTTTTCCATTTCCAGATAATG
ACATGCTTTCTTTTGCACTGACTCAAGCTCGTAAACCTTCTTCAAGCTTGATTTATCTTACTGCAACAACCACCGATAAT
TTGGAAAGACAAGTCAAGTTGGGGCAAATCGAAAAGTTACAACTTCCCCGACGTTTTCATGGTTTTCCATTGGTACTCCC
ACAATTTTTTTGGCAGAGCAAGTTTTATAAAATGGTCAAAAAACAACGCGAATCTGGTTTCCCATTACTTATCTTTGTCC
CTGAAATAAGACTGGGAGAAAAACTGAACCAAGACTTACAGAGTAGTTTTCCTCATGAAAAAATTGCCTTTGTTGCTTCA
ACGAGTCTTGAACGCTTAGAGGCTGTGGAACGTTTTCGCCAGGGCAATATTTCTATCCTTGTCTCAACGACTATTTTAGA
ACGTGGTGTAACTTTTCCTAAGGTTGACGTTTTTGTATTTCAAAGTCATCACCATAATTTCACAAGATCAAGCCTCATCC
AAATTGCTGGACGAGTAGGTCGAAGTACCGAAAGACCTGACGGCAAAGTGTTTTTCTTTCATTTAGGAAAAATAACAGCA
ATGTTGGAAGCTTATAAAAATATCAGAAATATGAATAAGGCAGGAGGTTTCCAATGA

Domains

Predicted by InterproScan.

• Type III restriction enzyme, res subunit (ResIII) (97-236)

• Helicase conserved C-terminal domain (Helicase_C) (274-382)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Lactococcus lactis subsp. cremoris KW2	57.398	93.78	0.538
comFA/cflA	Streptococcus mitis SK321	51.288	100	0.524
comFA/cflA	Streptococcus pneumoniae TIGR4	50.82	100	0.519
comFA/cflA	Streptococcus pneumoniae Rx1	50.82	100	0.519
comFA/cflA	Streptococcus pneumoniae D39	50.82	100	0.519
comFA/cflA	Streptococcus pneumoniae R6	50.82	100	0.519
comFA/cflA	Streptococcus mitis NCTC 12261	50.351	100	0.514
comFA	Bacillus subtilis subsp. subtilis str. 168	37.443	100	0.392
comFA	Latilactobacillus sakei subsp. sakei 23K	38.791	94.976	0.368