Detailed information NTG0918559

Bioinformatically predicted

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: VNN52_RS10275
• Genome accession: NZ_CP141701
• Coordinates: 1981959..1983215 (+)
• Length: 418 a.a.
• NCBI ID: WP_153924719.1
• Uniprot ID: -
• Organism: Lactococcus petauri strain M18012501 FF2g6
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1976959..1988215

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
VNN52_RS10250 (VNN52_10225)	glyQ	1977110..1978078 (-)	969	WP_019292765.1	glycine--tRNA ligase subunit alpha	-
VNN52_RS10255 (VNN52_10230)	-	1978092..1978871 (-)	780	WP_019292766.1	formate/nitrite transporter family protein	-
VNN52_RS10260 (VNN52_10235)	-	1979189..1980211 (+)	1023	WP_153924725.1	alpha/beta hydrolase	-
VNN52_RS10265 (VNN52_10240)	-	1980246..1981268 (-)	1023	WP_153924722.1	DUF475 domain-containing protein	-
VNN52_RS10270 (VNN52_10245)	-	1981281..1981904 (-)	624	WP_019292769.1	YigZ family protein	-
VNN52_RS10275 (VNN52_10250)	comFA	1981959..1983215 (+)	1257	WP_153924719.1	DEAD/DEAH box helicase	Machinery gene
VNN52_RS10280 (VNN52_10255)	-	1983212..1983856 (+)	645	WP_019292771.1	ComF family protein	-
VNN52_RS10285 (VNN52_10260)	-	1983859..1984320 (-)	462	WP_019292772.1	diacylglycerol kinase family protein	-
VNN52_RS10290 (VNN52_10265)	ybeY	1984304..1984792 (-)	489	WP_019292773.1	rRNA maturation RNase YbeY	-
VNN52_RS10295 (VNN52_10270)	-	1984802..1985275 (-)	474	WP_019292774.1	NUDIX hydrolase	-
VNN52_RS10300 (VNN52_10275)	-	1985277..1986236 (-)	960	WP_153924716.1	PhoH family protein	-
VNN52_RS10305 (VNN52_10280)	-	1986356..1987450 (-)	1095	WP_153924713.1	alanine dehydrogenase	-

Sequence

Protein

• Length: 418 a.a.
• Molecular weight: 47922.80 Da
• Isoelectric Point: 10.1309

>NTDB_id=918559 VNN52_RS10275 WP_153924719.1 1981959..1983215(+) (comFA) [Lactococcus petauri strain M18012501 FF2g6]
MNELYGRLLLQKELSKIPDKATLFDSMHDISRTVIMCNRCGKKSKKKEVLLPVGAYYCPHCIQMGRVRSDEKLYHLPQED
FLAASFLNWQGKLTAPQQYISDTLVKLHQQQKTVLVQAVTGAGKTEMIYQMIDNTLKKGKAVALTSPRIDVCLELYYRLK
RDFSCPISLLHGKSEKYSRSPLVIATTHQLMSFRHAFDLLILDEVDAFPFPDNDMLSFALTQARKPSSSLIYLTATTTDN
LERQVKLGQIEKLQLPRRFHGFPLVLPQFFWQSKFYKMVKKQRESGFPLLIFVPEIRLGEKLNQDLQSSFPHEKIAFVAS
TSLERLEAVERFRQGNISILVSTTILERGVTFPKVDVFVFQSHHHNFTRSSLIQIAGRVGRSTERPDGKVFFFHLGKITA
MLEAYKNIRNINKAGGFQ

Nucleotide

• Length: 1257 bp

>NTDB_id=918559 VNN52_RS10275 WP_153924719.1 1981959..1983215(+) (comFA) [Lactococcus petauri strain M18012501 FF2g6]
ATGAATGAATTATATGGTAGACTCCTTTTACAAAAAGAATTGTCGAAAATCCCAGACAAAGCAACACTTTTTGATAGTAT
GCACGATATTTCAAGAACAGTGATTATGTGCAATCGCTGTGGCAAAAAAAGTAAGAAAAAAGAGGTCCTACTCCCCGTTG
GTGCTTATTATTGTCCCCATTGTATTCAGATGGGACGTGTTCGCTCGGATGAAAAACTTTATCATCTTCCTCAGGAGGAT
TTTTTAGCAGCTTCTTTTCTAAACTGGCAGGGAAAACTCACAGCCCCTCAGCAATATATATCTGATACTCTAGTCAAATT
ACATCAGCAACAAAAAACGGTGCTGGTACAGGCTGTCACGGGTGCAGGAAAAACTGAAATGATTTATCAGATGATTGATA
ACACCCTTAAAAAAGGAAAAGCTGTAGCTTTAACTAGCCCTCGTATTGATGTCTGTCTAGAACTCTACTATCGCTTAAAG
CGAGATTTTTCTTGTCCCATCTCGCTTTTACACGGAAAAAGTGAGAAATACAGCCGCTCGCCACTCGTAATTGCGACGAC
ACATCAGCTTATGAGTTTTCGTCATGCCTTTGACTTACTCATTCTTGATGAGGTTGATGCTTTTCCATTTCCAGATAATG
ACATGCTTTCTTTTGCACTGACTCAAGCTCGTAAACCTTCTTCAAGCTTGATTTATCTTACTGCAACAACCACCGATAAT
TTGGAAAGACAAGTCAAGTTGGGGCAAATCGAAAAGTTACAACTTCCCCGACGTTTTCATGGTTTTCCATTGGTACTCCC
ACAATTTTTTTGGCAGAGCAAGTTTTATAAAATGGTCAAAAAACAACGCGAATCTGGTTTCCCATTACTTATCTTTGTCC
CTGAAATAAGACTGGGAGAAAAACTGAACCAAGACTTACAGAGTAGTTTTCCTCATGAAAAAATTGCCTTTGTTGCTTCA
ACGAGTCTTGAACGCTTAGAGGCTGTGGAACGTTTTCGCCAGGGCAATATTTCTATCCTTGTCTCAACGACTATTTTAGA
ACGTGGTGTAACTTTTCCTAAGGTTGACGTTTTTGTATTTCAAAGTCATCACCATAATTTCACAAGATCAAGCCTCATCC
AAATTGCTGGACGAGTAGGTCGAAGTACCGAAAGACCTGACGGCAAAGTGTTTTTCTTTCATTTAGGAAAAATAACAGCA
ATGTTGGAAGCTTATAAAAATATCAGAAATATAAATAAGGCAGGAGGTTTCCAATGA

Domains

Predicted by InterproScan.

• Type III restriction enzyme, res subunit (ResIII) (97-236)

• Helicase conserved C-terminal domain (Helicase_C) (274-382)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Lactococcus lactis subsp. cremoris KW2	56.888	93.78	0.533
comFA/cflA	Streptococcus mitis SK321	51.054	100	0.522
comFA/cflA	Streptococcus pneumoniae TIGR4	50.585	100	0.517
comFA/cflA	Streptococcus pneumoniae Rx1	50.585	100	0.517
comFA/cflA	Streptococcus pneumoniae D39	50.585	100	0.517
comFA/cflA	Streptococcus pneumoniae R6	50.585	100	0.517
comFA/cflA	Streptococcus mitis NCTC 12261	50.117	100	0.512
comFA	Bacillus subtilis subsp. subtilis str. 168	36.986	100	0.388
comFA	Latilactobacillus sakei subsp. sakei 23K	38.791	94.976	0.368