Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   VNN27_RS09400 Genome accession   NZ_CP141700
Coordinates   1951193..1952449 (+) Length   418 a.a.
NCBI ID   WP_346349940.1    Uniprot ID   -
Organism   Lactococcus petauri strain R22-25 IC35     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1946193..1957449
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VNN27_RS09375 (VNN27_09375) glyQ 1946344..1947312 (-) 969 WP_165719545.1 glycine--tRNA ligase subunit alpha -
  VNN27_RS09380 (VNN27_09380) - 1947326..1948105 (-) 780 WP_019292766.1 formate/nitrite transporter family protein -
  VNN27_RS09385 (VNN27_09385) - 1948423..1949445 (+) 1023 WP_165719544.1 alpha/beta hydrolase -
  VNN27_RS09390 (VNN27_09390) - 1949480..1950502 (-) 1023 WP_019293557.1 DUF475 domain-containing protein -
  VNN27_RS09395 (VNN27_09395) - 1950515..1951138 (-) 624 WP_019292769.1 YigZ family protein -
  VNN27_RS09400 (VNN27_09400) comFA 1951193..1952449 (+) 1257 WP_346349940.1 DEAD/DEAH box helicase Machinery gene
  VNN27_RS09405 (VNN27_09405) - 1952446..1953090 (+) 645 WP_346349941.1 ComF family protein -
  VNN27_RS09410 (VNN27_09410) - 1953093..1953554 (-) 462 WP_019292772.1 diacylglycerol kinase family protein -
  VNN27_RS09415 (VNN27_09415) ybeY 1953538..1954026 (-) 489 WP_019292773.1 rRNA maturation RNase YbeY -
  VNN27_RS09420 (VNN27_09420) - 1954036..1954509 (-) 474 WP_198494734.1 NUDIX hydrolase -
  VNN27_RS09425 (VNN27_09425) - 1954511..1955470 (-) 960 WP_004257953.1 PhoH family protein -
  VNN27_RS09430 (VNN27_09430) ald 1955590..1956696 (-) 1107 WP_346349942.1 alanine dehydrogenase -
  VNN27_RS09435 (VNN27_09435) - 1957006..1957347 (+) 342 WP_243418909.1 AAA family ATPase -

Sequence

Protein


Download         Length: 418 a.a.        Molecular weight: 47911.79 Da        Isoelectric Point: 10.1403

>NTDB_id=918513 VNN27_RS09400 WP_346349940.1 1951193..1952449(+) (comFA) [Lactococcus petauri strain R22-25 IC35]
MNELYGRLLLQKELSKIPDKAILFDSMHDISRTVIMCNRCGKKSKKKEVLLPVGAYYCPHCIQMGRVRSDGKLYHLPQED
FLAASFLNWQGKLTAPQQYISDTLVKLHQQQKTVLVQAVTGAGKTEMIYQMIDNTLKKGKAVALTSPRIDVCLELYYRLK
RDFSCPISLLHGKSEKYSRSPLVIATTHQLMRFRHAFDLLILDEVDAFPFPDNDMLSFALTQARKPSSSLIYLTATTTDN
LERQVKLGQIEKLQLPRRFHGFPLVLPQFFWQSKFYKMVKKQRESGFPLLIFVPEIRLGEKLSQDLQSSFPHEEIAFVAS
TSLERLEAVERFRQGNISILVSTTILERGVTFPKVDVFVFQSHHHNFTRSSLIQIAGRVGRSTERPDGKVFFFHLGKTTA
MLEAYKNIRNMNKAGGFQ

Nucleotide


Download         Length: 1257 bp        

>NTDB_id=918513 VNN27_RS09400 WP_346349940.1 1951193..1952449(+) (comFA) [Lactococcus petauri strain R22-25 IC35]
ATGAATGAATTATATGGTAGACTCCTTTTACAAAAAGAATTGTCGAAAATCCCAGACAAAGCAATACTTTTTGATAGTAT
GCACGATATTTCAAGAACAGTGATTATGTGCAATCGCTGTGGCAAAAAAAGTAAGAAAAAAGAGGTCCTACTCCCCGTTG
GTGCTTATTATTGTCCCCATTGTATTCAGATGGGACGTGTTCGCTCGGATGGAAAACTTTATCATCTTCCTCAGGAGGAT
TTTTTAGCAGCTTCTTTTCTAAACTGGCAGGGAAAACTCACAGCCCCTCAGCAATATATATCTGATACTCTAGTCAAATT
ACATCAGCAACAAAAAACGGTGCTGGTACAGGCTGTCACGGGTGCAGGAAAAACTGAAATGATTTATCAGATGATTGATA
ACACCCTTAAAAAAGGAAAAGCTGTAGCTTTAACTAGCCCTCGTATTGATGTCTGTCTAGAACTCTACTATCGCTTAAAG
CGAGATTTTTCTTGTCCCATCTCGCTTTTACACGGAAAAAGTGAGAAATACAGCCGCTCGCCACTCGTAATTGCGACGAC
ACATCAGCTTATGCGTTTTCGTCATGCCTTTGACTTACTCATTCTTGATGAGGTTGATGCTTTTCCATTTCCAGATAATG
ACATGCTTTCTTTTGCACTGACTCAAGCTCGTAAACCTTCTTCAAGCTTGATTTATCTTACTGCAACAACCACCGATAAT
TTGGAAAGACAAGTCAAGTTGGGGCAAATCGAAAAGTTACAACTTCCCCGACGTTTTCATGGTTTTCCATTGGTACTCCC
ACAATTTTTTTGGCAGAGCAAGTTTTATAAAATGGTCAAAAAACAACGCGAATCTGGTTTCCCATTACTTATCTTTGTCC
CTGAAATAAGACTGGGAGAAAAACTGAGCCAAGACTTACAGAGTAGTTTTCCTCATGAAGAAATTGCCTTTGTTGCTTCA
ACGAGTCTTGAACGCTTAGAGGCTGTGGAACGTTTTCGTCAGGGCAATATTTCTATCCTTGTCTCAACGACTATTTTAGA
ACGTGGTGTAACTTTTCCTAAGGTTGACGTTTTTGTATTTCAAAGTCATCACCATAATTTCACAAGATCAAGCCTCATCC
AAATTGCCGGACGAGTAGGTCGAAGTACCGAAAGACCTGATGGCAAAGTGTTTTTCTTTCATTTAGGAAAAACAACAGCA
ATGTTGGAAGCTTATAAAAATATCAGAAATATGAATAAGGCAGGAGGTTTCCAATGA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(97-236)

Helicase conserved C-terminal domain (Helicase_C)

(274-382)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Lactococcus lactis subsp. cremoris KW2

56.391

95.455

0.538
  comFA/cflA Streptococcus mitis SK321

51.054

100

0.522
  comFA/cflA Streptococcus mitis NCTC 12261

50.117

100

0.512
  comFA/cflA Streptococcus pneumoniae R6

53.03

94.737

0.502
  comFA/cflA Streptococcus pneumoniae D39

53.03

94.737

0.502
  comFA/cflA Streptococcus pneumoniae TIGR4

53.03

94.737

0.502
  comFA/cflA Streptococcus pneumoniae Rx1

53.03

94.737

0.502
  comFA Bacillus subtilis subsp. subtilis str. 168

37.9

100

0.397
  comFA Latilactobacillus sakei subsp. sakei 23K

39.043

94.976

0.371