Detailed information NTG0918468

Bioinformatically predicted

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: VNN49_RS09515
• Genome accession: NZ_CP141699
• Coordinates: 1973552..1974808 (+)
• Length: 418 a.a.
• NCBI ID: WP_338963432.1
• Uniprot ID: -
• Organism: Lactococcus petauri strain M17100408 FF4i7
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1968552..1979808

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
VNN49_RS09490 (VNN49_09490)	glyQ	1968703..1969671 (-)	969	WP_019292765.1	glycine--tRNA ligase subunit alpha	-
VNN49_RS09495 (VNN49_09495)	-	1969685..1970464 (-)	780	WP_019292766.1	formate/nitrite transporter family protein	-
VNN49_RS09500 (VNN49_09500)	-	1970782..1971804 (+)	1023	WP_019293556.1	alpha/beta hydrolase	-
VNN49_RS09505 (VNN49_09505)	-	1971839..1972861 (-)	1023	WP_019293557.1	DUF475 domain-containing protein	-
VNN49_RS09510 (VNN49_09510)	-	1972874..1973497 (-)	624	WP_019293558.1	YigZ family protein	-
VNN49_RS09515 (VNN49_09515)	comFA	1973552..1974808 (+)	1257	WP_338963432.1	DEAD/DEAH box helicase	Machinery gene
VNN49_RS09520 (VNN49_09520)	-	1974805..1975449 (+)	645	WP_019293560.1	ComF family protein	-
VNN49_RS09525 (VNN49_09525)	-	1975452..1975913 (-)	462	WP_019292772.1	diacylglycerol kinase family protein	-
VNN49_RS09530 (VNN49_09530)	ybeY	1975897..1976385 (-)	489	WP_019293561.1	rRNA maturation RNase YbeY	-
VNN49_RS09535 (VNN49_09535)	-	1976395..1976868 (-)	474	WP_019292774.1	NUDIX hydrolase	-
VNN49_RS09540 (VNN49_09540)	-	1976870..1977829 (-)	960	WP_004257953.1	PhoH family protein	-
VNN49_RS09545 (VNN49_09545)	ald	1977949..1979055 (-)	1107	WP_338963435.1	alanine dehydrogenase	-
VNN49_RS09550 (VNN49_09550)	-	1979365..1979646 (+)	282	WP_227671623.1	hypothetical protein	-

Sequence

Protein

• Length: 418 a.a.
• Molecular weight: 48016.88 Da
• Isoelectric Point: 10.1309

>NTDB_id=918468 VNN49_RS09515 WP_338963432.1 1973552..1974808(+) (comFA) [Lactococcus petauri strain M17100408 FF4i7]
MNELYGRLLLQKELSKIPDKAILFDSMHDISRTVIMCNRCGKKSKKKEVLLPVGAYYCPHCIQMGRVRSDEKLYHLPQED
FFTASFLNWQGKLTPPQQYISDTLVKLHQQQKTVLVQAVTGAGKTEMIYQMIDNTLKKGKAVGLTSPRIDVCLELYYRLK
RDFSCPISLLHGKSEKYSRSPLVIATTHQLMRFRHAFDLVILDEVDAFPFPDNDMLSFALTQARKPSSSLIYLTATTTNN
LEKQVKLGQIEKLQLPRRFHGFPLVLPQFFWQSKFYKMVKKQRESGFPLLIFVPEIRLGEKLSQDLQSSFPHEEIAFVAS
TSLERLEAVERFRQGNISILVSTTILERGVTFPKVDVFVFQSHHHNFTRSSLIQIAGRVGRSTERPDGKVFFFHLGKTTA
MLEAYKNIRNMNKAGGFQ

Nucleotide

• Length: 1257 bp

>NTDB_id=918468 VNN49_RS09515 WP_338963432.1 1973552..1974808(+) (comFA) [Lactococcus petauri strain M17100408 FF4i7]
ATGAATGAATTATATGGTAGACTCCTTTTACAAAAAGAATTGTCGAAAATCCCAGACAAAGCAATACTTTTTGATAGTAT
GCACGATATTTCAAGAACAGTGATTATGTGCAATCGCTGCGGTAAAAAAAGTAAGAAAAAAGAGGTCTTACTCCCCGTTG
GTGCTTATTATTGTCCCCATTGTATTCAGATGGGACGTGTTCGCTCGGATGAAAAACTTTATCACCTTCCTCAGGAGGAT
TTTTTCACAGCTTCTTTTCTAAACTGGCAGGGAAAACTCACACCCCCTCAGCAATATATATCTGATACTCTAGTCAAATT
ACATCAGCAACAAAAAACGGTGCTGGTGCAGGCTGTCACGGGTGCAGGAAAAACTGAAATGATTTATCAGATGATTGATA
ACACCCTTAAAAAAGGAAAAGCTGTAGGTTTAACTAGTCCTCGTATTGATGTCTGTCTAGAACTCTACTATCGCTTAAAG
CGAGATTTTTCTTGTCCCATCTCGCTTTTACACGGAAAAAGTGAGAAATACAGCCGCTCGCCACTCGTAATTGCGACGAC
ACATCAGCTTATGCGTTTTCGTCATGCCTTTGACTTAGTCATTCTTGATGAGGTTGATGCTTTTCCATTTCCAGATAATG
ACATGCTCTCTTTTGCACTGACTCAAGCTCGTAAACCTTCTTCAAGCTTGATTTATCTTACTGCAACAACCACCAATAAT
TTGGAAAAACAAGTCAAGTTGGGGCAAATCGAAAAGTTACAACTTCCCCGACGTTTTCATGGTTTTCCATTGGTACTCCC
GCAATTTTTTTGGCAGAGCAAGTTTTACAAAATGGTCAAAAAACAACGCGAATCTGGTTTCCCATTACTTATCTTTGTCC
CTGAAATAAGACTGGGAGAAAAACTGAGCCAAGACTTACAGAGTAGTTTTCCTCATGAAGAAATTGCCTTTGTTGCTTCA
ACGAGTCTTGAACGCTTAGAGGCTGTGGAACGTTTTCGTCAGGGCAATATTTCTATCCTTGTCTCAACGACTATTTTAGA
ACGTGGTGTAACTTTTCCTAAGGTTGACGTTTTTGTATTTCAAAGTCATCACCATAATTTCACAAGATCAAGCCTCATCC
AAATTGCCGGACGAGTAGGTCGAAGTACCGAAAGACCTGATGGCAAAGTGTTTTTCTTTCATTTAGGAAAAACAACAGCA
ATGTTGGAAGCTTATAAAAATATCAGAAATATGAATAAGGCAGGAGGTTTCCAATGA

Domains

Predicted by InterproScan.

• Helicase conserved C-terminal domain (Helicase_C) (274-382)

• Type III restriction enzyme, res subunit (ResIII) (93-236)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Lactococcus lactis subsp. cremoris KW2	56.642	95.455	0.541
comFA/cflA	Streptococcus mitis SK321	50.585	100	0.517
comFA/cflA	Streptococcus pneumoniae Rx1	53.535	94.737	0.507
comFA/cflA	Streptococcus pneumoniae D39	53.535	94.737	0.507
comFA/cflA	Streptococcus pneumoniae R6	53.535	94.737	0.507
comFA/cflA	Streptococcus pneumoniae TIGR4	53.535	94.737	0.507
comFA/cflA	Streptococcus mitis NCTC 12261	52.02	94.737	0.493
comFA	Bacillus subtilis subsp. subtilis str. 168	37.671	100	0.395
comFA	Latilactobacillus sakei subsp. sakei 23K	39.547	94.976	0.376