Detailed information NTG0918378

Bioinformatically predicted

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: VNN38_RS09770
• Genome accession: NZ_CP141697
• Coordinates: 2028734..2029990 (+)
• Length: 418 a.a.
• NCBI ID: WP_019293559.1
• Uniprot ID: -
• Organism: Lactococcus petauri strain R21-77-2
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2023734..2034990

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
VNN38_RS09745 (VNN38_09745)	glyQ	2023885..2024853 (-)	969	WP_019292765.1	glycine--tRNA ligase subunit alpha	-
VNN38_RS09750 (VNN38_09750)	-	2024867..2025646 (-)	780	WP_019292766.1	formate/nitrite transporter family protein	-
VNN38_RS09755 (VNN38_09755)	-	2025964..2026986 (+)	1023	WP_019293556.1	alpha/beta hydrolase	-
VNN38_RS09760 (VNN38_09760)	-	2027021..2028043 (-)	1023	WP_019293557.1	DUF475 domain-containing protein	-
VNN38_RS09765 (VNN38_09765)	-	2028056..2028679 (-)	624	WP_338955103.1	YigZ family protein	-
VNN38_RS09770 (VNN38_09770)	comFA	2028734..2029990 (+)	1257	WP_019293559.1	DEAD/DEAH box helicase	Machinery gene
VNN38_RS09775 (VNN38_09775)	-	2029987..2030631 (+)	645	WP_019293560.1	ComF family protein	-
VNN38_RS09780 (VNN38_09780)	-	2030634..2031095 (-)	462	WP_019292772.1	diacylglycerol kinase family protein	-
VNN38_RS09785 (VNN38_09785)	ybeY	2031079..2031567 (-)	489	WP_338955107.1	rRNA maturation RNase YbeY	-
VNN38_RS09790 (VNN38_09790)	-	2031577..2032050 (-)	474	WP_019292774.1	NUDIX hydrolase	-
VNN38_RS09795 (VNN38_09795)	-	2032052..2033011 (-)	960	WP_004257953.1	PhoH family protein	-
VNN38_RS09800 (VNN38_09800)	ald	2033131..2034237 (-)	1107	WP_019293562.1	alanine dehydrogenase	-
VNN38_RS09805 (VNN38_09805)	-	2034547..2034828 (+)	282	WP_227671623.1	hypothetical protein	-

Sequence

Protein

• Length: 418 a.a.
• Molecular weight: 47952.84 Da
• Isoelectric Point: 10.1309

>NTDB_id=918378 VNN38_RS09770 WP_019293559.1 2028734..2029990(+) (comFA) [Lactococcus petauri strain R21-77-2]
MNELYGRLLLQKELSKIPDKAILFDSMHDISRTVIMCNRCGKKSKKKEVLLPVGAYYCPHCIQMGRVRSDEKLYHLPQED
FLAASFLNWQGKLTPPQQYISDTLVKLHQQQKTVLVQAVTGAGKTEMIYQMIDNTLKKGKAVGLTSPRIDVCLELYYRLK
RDFSCPISLLHGKSEKYSRSPLVIATTHQLMRFRHAFDLVILDEVDAFPFPDNDMLSFALTQARKPSSSLIYLTATTTNN
LEKQVKLGQIEKLQLPRRFHGFPLVLPQFFWQSKFYKMVKKQRESGFPLLIFVPEIRLGEKLSQDLQSSFPHEEIAFVAS
TSLERLEAVERFRQGNISILVSTTILERGVTFPKVDVFVFQSHHHNFTRSSLIQIAGRVGRSTERPDGKVFFFHLGKTTA
MLEAYKNIRNMNKAGGFQ

Nucleotide

• Length: 1257 bp

>NTDB_id=918378 VNN38_RS09770 WP_019293559.1 2028734..2029990(+) (comFA) [Lactococcus petauri strain R21-77-2]
ATGAATGAATTATATGGTAGACTCCTTTTACAAAAAGAATTGTCGAAAATCCCAGACAAAGCAATACTTTTTGATAGTAT
GCACGATATTTCAAGAACAGTGATTATGTGCAATCGCTGCGGTAAAAAAAGTAAGAAAAAAGAGGTCTTACTCCCCGTTG
GTGCTTATTATTGTCCCCATTGTATTCAGATGGGACGTGTTCGCTCGGATGAAAAACTTTATCACCTTCCTCAGGAGGAT
TTTTTAGCAGCTTCTTTTCTAAACTGGCAGGGAAAACTCACACCCCCTCAGCAATATATATCTGATACTCTAGTCAAATT
ACATCAGCAACAAAAAACGGTGCTGGTGCAGGCTGTCACGGGTGCAGGAAAAACTGAAATGATTTATCAGATGATTGATA
ACACCCTTAAAAAAGGAAAAGCTGTAGGTTTAACTAGTCCTCGTATTGATGTCTGTCTAGAACTCTACTATCGCTTAAAG
CGAGATTTTTCTTGTCCCATCTCGCTTTTACACGGAAAAAGTGAGAAATACAGCCGCTCGCCACTCGTAATTGCGACGAC
ACATCAGCTTATGCGTTTTCGTCATGCCTTTGACTTAGTCATTCTTGATGAGGTTGATGCTTTTCCATTTCCAGATAATG
ACATGCTCTCTTTTGCACTGACTCAAGCTCGTAAACCTTCTTCAAGCTTGATTTATCTTACTGCAACAACCACCAATAAT
TTGGAAAAACAAGTCAAGTTGGGGCAAATCGAAAAGTTACAACTTCCCCGACGTTTTCATGGTTTTCCATTGGTACTCCC
GCAATTTTTTTGGCAGAGCAAGTTTTACAAAATGGTCAAAAAACAACGCGAATCTGGTTTCCCATTACTTATCTTTGTCC
CTGAAATAAGACTGGGAGAAAAACTGAGCCAAGACTTACAGAGTAGTTTTCCTCATGAAGAAATTGCCTTTGTTGCTTCA
ACGAGTCTTGAACGCTTAGAGGCTGTGGAACGTTTTCGTCAGGGCAATATTTCTATCCTTGTCTCAACGACTATTTTAGA
ACGTGGTGTAACTTTTCCTAAGGTTGACGTTTTTGTATTTCAAAGTCATCACCATAATTTCACAAGATCAAGCCTCATCC
AAATTGCCGGACGAGTAGGTCGAAGTACCGAAAGACCTGATGGCAAAGTGTTTTTCTTTCATTTAGGAAAAACAACAGCA
ATGTTGGAAGCTTATAAAAATATCAGAAATATGAATAAGGCAGGAGGTTTCCAATGA

Domains

Predicted by InterproScan.

• Type III restriction enzyme, res subunit (ResIII) (93-236)

• Helicase conserved C-terminal domain (Helicase_C) (274-382)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Lactococcus lactis subsp. cremoris KW2	56.642	95.455	0.541
comFA/cflA	Streptococcus mitis SK321	50.585	100	0.517
comFA/cflA	Streptococcus pneumoniae Rx1	53.535	94.737	0.507
comFA/cflA	Streptococcus pneumoniae D39	53.535	94.737	0.507
comFA/cflA	Streptococcus pneumoniae R6	53.535	94.737	0.507
comFA/cflA	Streptococcus pneumoniae TIGR4	53.535	94.737	0.507
comFA/cflA	Streptococcus mitis NCTC 12261	52.02	94.737	0.493
comFA	Bacillus subtilis subsp. subtilis str. 168	37.671	100	0.395
comFA	Latilactobacillus sakei subsp. sakei 23K	39.295	94.976	0.373