Detailed information NTG0918243

Bioinformatically predicted

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: VNN26_RS09820
• Genome accession: NZ_CP141690
• Coordinates: 1996002..1997258 (+)
• Length: 418 a.a.
• NCBI ID: WP_338953540.1
• Uniprot ID: -
• Organism: Lactococcus petauri strain R22-11 BR-C
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1991002..2002258

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
VNN26_RS09795 (VNN26_09795)	glyQ	1991153..1992121 (-)	969	WP_165719545.1	glycine--tRNA ligase subunit alpha	-
VNN26_RS09800 (VNN26_09800)	-	1992135..1992914 (-)	780	WP_019292766.1	formate/nitrite transporter family protein	-
VNN26_RS09805 (VNN26_09805)	-	1993232..1994254 (+)	1023	WP_034999659.1	alpha/beta hydrolase	-
VNN26_RS09810 (VNN26_09810)	-	1994289..1995311 (-)	1023	WP_019293557.1	DUF475 domain-containing protein	-
VNN26_RS09815 (VNN26_09815)	-	1995324..1995947 (-)	624	WP_019292769.1	YigZ family protein	-
VNN26_RS09820 (VNN26_09820)	comFA	1996002..1997258 (+)	1257	WP_338953540.1	DEAD/DEAH box helicase	Machinery gene
VNN26_RS09825 (VNN26_09825)	-	1997255..1997899 (+)	645	WP_206888836.1	ComF family protein	-
VNN26_RS09830 (VNN26_09830)	-	1997902..1998363 (-)	462	WP_019292772.1	diacylglycerol kinase family protein	-
VNN26_RS09835 (VNN26_09835)	ybeY	1998347..1998835 (-)	489	WP_019292773.1	rRNA maturation RNase YbeY	-
VNN26_RS09840 (VNN26_09840)	-	1998845..1999318 (-)	474	WP_198494734.1	NUDIX hydrolase	-
VNN26_RS09845 (VNN26_09845)	-	1999320..2000279 (-)	960	WP_004257953.1	PhoH family protein	-
VNN26_RS09850 (VNN26_09850)	ald	2000399..2001505 (-)	1107	WP_206888834.1	alanine dehydrogenase	-

Sequence

Protein

• Length: 418 a.a.
• Molecular weight: 48040.91 Da
• Isoelectric Point: 10.1835

>NTDB_id=918243 VNN26_RS09820 WP_338953540.1 1996002..1997258(+) (comFA) [Lactococcus petauri strain R22-11 BR-C]
MNELYGRLLLQKELSKIPDKATLFDSMHDISRTVIMCNRCGKKSKKKEVLLPVGAYYCPHCIQMGRVRSDEKLYHLPQED
FLAASFLNWQGKLTAPQQYISDTLVKLHQQQKTVLVQAVTGAGKTEMIYQMIDNTLKKGKAVGLTSPRIDVCLELYYRLK
RDFSCPISLLHGKSEKYSRSPLVIATTHQLMRFRHAFDLVILDEVDAFPFPDNDMLSFALTQARKPSSSLIYLTATTTNN
LEKQVKLGQIEKLQLPRRFHGFPLVLPQFFWQSKFYKMVKKQRESGFPLLIFVPEIRLGEKLNQDLQSSFPHEEIAFVAS
TSLERLEAVERFRQGNISILVSTTILERGVTFPKVDVFVFQSHHHNFTRSSLIQIARRVGRSTERPDGKVFFFHLGKTTA
MLEAYKNIRNMNKAGGFQ

Nucleotide

• Length: 1257 bp

>NTDB_id=918243 VNN26_RS09820 WP_338953540.1 1996002..1997258(+) (comFA) [Lactococcus petauri strain R22-11 BR-C]
ATGAATGAATTATATGGTAGACTCCTTTTACAAAAAGAATTGTCGAAAATCCCAGACAAAGCAACACTTTTTGATAGTAT
GCACGATATTTCAAGAACAGTGATTATGTGCAATCGCTGTGGCAAAAAAAGTAAGAAAAAAGAGGTCCTACTCCCCGTTG
GTGCTTATTATTGTCCCCATTGTATTCAGATGGGACGTGTTCGCTCGGATGAAAAACTTTATCATCTTCCTCAGGAGGAT
TTTTTAGCAGCTTCTTTTCTAAACTGGCAGGGAAAACTCACAGCCCCTCAGCAATATATATCTGATACTCTAGTCAAATT
ACATCAGCAACAAAAAACGGTGCTGGTGCAGGCTGTCACGGGTGCAGGAAAAACTGAAATGATTTATCAGATGATTGATA
ACACCCTTAAAAAAGGAAAAGCTGTAGGTTTAACTAGTCCTCGTATTGATGTCTGTCTAGAACTCTACTATCGCTTAAAG
CGAGATTTTTCTTGTCCCATCTCGCTTTTACACGGAAAAAGTGAGAAATACAGCCGCTCGCCACTCGTAATTGCGACGAC
ACATCAGCTTATGCGTTTTCGTCATGCCTTTGACTTAGTCATTCTTGATGAGGTTGATGCTTTTCCATTTCCAGATAATG
ACATGCTCTCTTTTGCACTGACTCAAGCTCGTAAACCTTCTTCAAGCTTGATTTATCTTACTGCAACAACCACCAATAAT
TTGGAAAAACAAGTCAAGTTGGGGCAAATCGAAAAGTTACAACTTCCCCGACGTTTTCATGGTTTTCCATTGGTACTCCC
ACAATTTTTTTGGCAGAGCAAGTTTTATAAAATGGTCAAAAAACAACGCGAATCTGGTTTCCCATTACTTATCTTTGTCC
CTGAAATAAGACTGGGAGAAAAACTGAACCAAGACTTACAGAGTAGTTTTCCTCATGAAGAAATTGCCTTTGTTGCTTCA
ACGAGTCTTGAACGCTTAGAGGCTGTGGAACGTTTTCGCCAGGGCAATATTTCTATCCTTGTCTCAACGACTATTTTAGA
ACGTGGTGTAACTTTTCCTAAGGTTGACGTTTTTGTATTTCAAAGTCATCACCATAATTTCACAAGATCAAGCCTCATCC
AAATTGCTAGACGAGTAGGTCGAAGTACCGAAAGACCTGACGGCAAAGTGTTTTTCTTTCATTTAGGAAAAACAACAGCA
ATGTTGGAAGCTTATAAAAATATCAGAAATATGAATAAGGCAGGAGGTTTCCAATGA

Domains

Predicted by InterproScan.

• Type III restriction enzyme, res subunit (ResIII) (97-236)

• Helicase conserved C-terminal domain (Helicase_C) (274-382)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Lactococcus lactis subsp. cremoris KW2	56.888	93.78	0.533
comFA/cflA	Streptococcus pneumoniae TIGR4	50.585	100	0.517
comFA/cflA	Streptococcus pneumoniae Rx1	50.585	100	0.517
comFA/cflA	Streptococcus pneumoniae D39	50.585	100	0.517
comFA/cflA	Streptococcus pneumoniae R6	50.585	100	0.517
comFA/cflA	Streptococcus mitis SK321	50.117	100	0.512
comFA/cflA	Streptococcus mitis NCTC 12261	49.18	100	0.502
comFA	Bacillus subtilis subsp. subtilis str. 168	37.215	100	0.39
comFA	Latilactobacillus sakei subsp. sakei 23K	38.791	94.976	0.368