Detailed information NTG0918198

Bioinformatically predicted

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: VNN44_RS09805
• Genome accession: NZ_CP141689
• Coordinates: 1996013..1997269 (+)
• Length: 418 a.a.
• NCBI ID: WP_338953540.1
• Uniprot ID: -
• Organism: Lactococcus petauri strain R22-16 FS-A5
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1991013..2002269

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
VNN44_RS09780 (VNN44_09780)	glyQ	1991164..1992132 (-)	969	WP_165719545.1	glycine--tRNA ligase subunit alpha	-
VNN44_RS09785 (VNN44_09785)	-	1992146..1992925 (-)	780	WP_019292766.1	formate/nitrite transporter family protein	-
VNN44_RS09790 (VNN44_09790)	-	1993243..1994265 (+)	1023	WP_034999659.1	alpha/beta hydrolase	-
VNN44_RS09795 (VNN44_09795)	-	1994300..1995322 (-)	1023	WP_019293557.1	DUF475 domain-containing protein	-
VNN44_RS09800 (VNN44_09800)	-	1995335..1995958 (-)	624	WP_019292769.1	YigZ family protein	-
VNN44_RS09805 (VNN44_09805)	comFA	1996013..1997269 (+)	1257	WP_338953540.1	DEAD/DEAH box helicase	Machinery gene
VNN44_RS09810 (VNN44_09810)	-	1997266..1997910 (+)	645	WP_206888836.1	ComF family protein	-
VNN44_RS09815 (VNN44_09815)	-	1997913..1998374 (-)	462	WP_019292772.1	diacylglycerol kinase family protein	-
VNN44_RS09820 (VNN44_09820)	ybeY	1998358..1998846 (-)	489	WP_019292773.1	rRNA maturation RNase YbeY	-
VNN44_RS09825 (VNN44_09825)	-	1998856..1999329 (-)	474	WP_198494734.1	NUDIX hydrolase	-
VNN44_RS09830 (VNN44_09830)	-	1999331..2000290 (-)	960	WP_004257953.1	PhoH family protein	-
VNN44_RS09835 (VNN44_09835)	ald	2000410..2001516 (-)	1107	WP_206888834.1	alanine dehydrogenase	-

Sequence

Protein

• Length: 418 a.a.
• Molecular weight: 48040.91 Da
• Isoelectric Point: 10.1835

>NTDB_id=918198 VNN44_RS09805 WP_338953540.1 1996013..1997269(+) (comFA) [Lactococcus petauri strain R22-16 FS-A5]
MNELYGRLLLQKELSKIPDKATLFDSMHDISRTVIMCNRCGKKSKKKEVLLPVGAYYCPHCIQMGRVRSDEKLYHLPQED
FLAASFLNWQGKLTAPQQYISDTLVKLHQQQKTVLVQAVTGAGKTEMIYQMIDNTLKKGKAVGLTSPRIDVCLELYYRLK
RDFSCPISLLHGKSEKYSRSPLVIATTHQLMRFRHAFDLVILDEVDAFPFPDNDMLSFALTQARKPSSSLIYLTATTTNN
LEKQVKLGQIEKLQLPRRFHGFPLVLPQFFWQSKFYKMVKKQRESGFPLLIFVPEIRLGEKLNQDLQSSFPHEEIAFVAS
TSLERLEAVERFRQGNISILVSTTILERGVTFPKVDVFVFQSHHHNFTRSSLIQIARRVGRSTERPDGKVFFFHLGKTTA
MLEAYKNIRNMNKAGGFQ

Nucleotide

• Length: 1257 bp

>NTDB_id=918198 VNN44_RS09805 WP_338953540.1 1996013..1997269(+) (comFA) [Lactococcus petauri strain R22-16 FS-A5]
ATGAATGAATTATATGGTAGACTCCTTTTACAAAAAGAATTGTCGAAAATCCCAGACAAAGCAACACTTTTTGATAGTAT
GCACGATATTTCAAGAACAGTGATTATGTGCAATCGCTGTGGCAAAAAAAGTAAGAAAAAAGAGGTCCTACTCCCCGTTG
GTGCTTATTATTGTCCCCATTGTATTCAGATGGGACGTGTTCGCTCGGATGAAAAACTTTATCATCTTCCTCAGGAGGAT
TTTTTAGCAGCTTCTTTTCTAAACTGGCAGGGAAAACTCACAGCCCCTCAGCAATATATATCTGATACTCTAGTCAAATT
ACATCAGCAACAAAAAACGGTGCTGGTGCAGGCTGTCACGGGTGCAGGAAAAACTGAAATGATTTATCAGATGATTGATA
ACACCCTTAAAAAAGGAAAAGCTGTAGGTTTAACTAGTCCTCGTATTGATGTCTGTCTAGAACTCTACTATCGCTTAAAG
CGAGATTTTTCTTGTCCCATCTCGCTTTTACACGGAAAAAGTGAGAAATACAGCCGCTCGCCACTCGTAATTGCGACGAC
ACATCAGCTTATGCGTTTTCGTCATGCCTTTGACTTAGTCATTCTTGATGAGGTTGATGCTTTTCCATTTCCAGATAATG
ACATGCTCTCTTTTGCACTGACTCAAGCTCGTAAACCTTCTTCAAGCTTGATTTATCTTACTGCAACAACCACCAATAAT
TTGGAAAAACAAGTCAAGTTGGGGCAAATCGAAAAGTTACAACTTCCCCGACGTTTTCATGGTTTTCCATTGGTACTCCC
ACAATTTTTTTGGCAGAGCAAGTTTTATAAAATGGTCAAAAAACAACGCGAATCTGGTTTCCCATTACTTATCTTTGTCC
CTGAAATAAGACTGGGAGAAAAACTGAACCAAGACTTACAGAGTAGTTTTCCTCATGAAGAAATTGCCTTTGTTGCTTCA
ACGAGTCTTGAACGCTTAGAGGCTGTGGAACGTTTTCGCCAGGGCAATATTTCTATCCTTGTCTCAACGACTATTTTAGA
ACGTGGTGTAACTTTTCCTAAGGTTGACGTTTTTGTATTTCAAAGTCATCACCATAATTTCACAAGATCAAGCCTCATCC
AAATTGCTAGACGAGTAGGTCGAAGTACCGAAAGACCTGACGGCAAAGTGTTTTTCTTTCATTTAGGAAAAACAACAGCA
ATGTTGGAAGCTTATAAAAATATCAGAAATATGAATAAGGCAGGAGGTTTCCAATGA

Domains

Predicted by InterproScan.

• Type III restriction enzyme, res subunit (ResIII) (97-236)

• Helicase conserved C-terminal domain (Helicase_C) (274-382)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Lactococcus lactis subsp. cremoris KW2	56.888	93.78	0.533
comFA/cflA	Streptococcus pneumoniae TIGR4	50.585	100	0.517
comFA/cflA	Streptococcus pneumoniae Rx1	50.585	100	0.517
comFA/cflA	Streptococcus pneumoniae D39	50.585	100	0.517
comFA/cflA	Streptococcus pneumoniae R6	50.585	100	0.517
comFA/cflA	Streptococcus mitis SK321	50.117	100	0.512
comFA/cflA	Streptococcus mitis NCTC 12261	49.18	100	0.502
comFA	Bacillus subtilis subsp. subtilis str. 168	37.215	100	0.39
comFA	Latilactobacillus sakei subsp. sakei 23K	38.791	94.976	0.368