Detailed information NTG0918019

Bioinformatically predicted

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: VNN43_RS09825
• Genome accession: NZ_CP141685
• Coordinates: 1995961..1997217 (+)
• Length: 418 a.a.
• NCBI ID: WP_338953540.1
• Uniprot ID: -
• Organism: Lactococcus petauri strain R21-69 HC
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1990961..2002217

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
VNN43_RS09800 (VNN43_09800)	glyQ	1991112..1992080 (-)	969	WP_165719545.1	glycine--tRNA ligase subunit alpha	-
VNN43_RS09805 (VNN43_09805)	-	1992094..1992873 (-)	780	WP_019292766.1	formate/nitrite transporter family protein	-
VNN43_RS09810 (VNN43_09810)	-	1993191..1994213 (+)	1023	WP_034999659.1	alpha/beta hydrolase	-
VNN43_RS09815 (VNN43_09815)	-	1994248..1995270 (-)	1023	WP_019293557.1	DUF475 domain-containing protein	-
VNN43_RS09820 (VNN43_09820)	-	1995283..1995906 (-)	624	WP_019292769.1	YigZ family protein	-
VNN43_RS09825 (VNN43_09825)	comFA	1995961..1997217 (+)	1257	WP_338953540.1	DEAD/DEAH box helicase	Machinery gene
VNN43_RS09830 (VNN43_09830)	-	1997214..1997858 (+)	645	WP_206888836.1	ComF family protein	-
VNN43_RS09835 (VNN43_09835)	-	1997861..1998322 (-)	462	WP_019292772.1	diacylglycerol kinase family protein	-
VNN43_RS09840 (VNN43_09840)	ybeY	1998306..1998794 (-)	489	WP_019292773.1	rRNA maturation RNase YbeY	-
VNN43_RS09845 (VNN43_09845)	-	1998804..1999277 (-)	474	WP_198494734.1	NUDIX hydrolase	-
VNN43_RS09850 (VNN43_09850)	-	1999279..2000238 (-)	960	WP_004257953.1	PhoH family protein	-
VNN43_RS09855 (VNN43_09855)	ald	2000358..2001464 (-)	1107	WP_206888834.1	alanine dehydrogenase	-

Sequence

Protein

• Length: 418 a.a.
• Molecular weight: 48040.91 Da
• Isoelectric Point: 10.1835

>NTDB_id=918019 VNN43_RS09825 WP_338953540.1 1995961..1997217(+) (comFA) [Lactococcus petauri strain R21-69 HC]
MNELYGRLLLQKELSKIPDKATLFDSMHDISRTVIMCNRCGKKSKKKEVLLPVGAYYCPHCIQMGRVRSDEKLYHLPQED
FLAASFLNWQGKLTAPQQYISDTLVKLHQQQKTVLVQAVTGAGKTEMIYQMIDNTLKKGKAVGLTSPRIDVCLELYYRLK
RDFSCPISLLHGKSEKYSRSPLVIATTHQLMRFRHAFDLVILDEVDAFPFPDNDMLSFALTQARKPSSSLIYLTATTTNN
LEKQVKLGQIEKLQLPRRFHGFPLVLPQFFWQSKFYKMVKKQRESGFPLLIFVPEIRLGEKLNQDLQSSFPHEEIAFVAS
TSLERLEAVERFRQGNISILVSTTILERGVTFPKVDVFVFQSHHHNFTRSSLIQIARRVGRSTERPDGKVFFFHLGKTTA
MLEAYKNIRNMNKAGGFQ

Nucleotide

• Length: 1257 bp

>NTDB_id=918019 VNN43_RS09825 WP_338953540.1 1995961..1997217(+) (comFA) [Lactococcus petauri strain R21-69 HC]
ATGAATGAATTATATGGTAGACTCCTTTTACAAAAAGAATTGTCGAAAATCCCAGACAAAGCAACACTTTTTGATAGTAT
GCACGATATTTCAAGAACAGTGATTATGTGCAATCGCTGTGGCAAAAAAAGTAAGAAAAAAGAGGTCCTACTCCCCGTTG
GTGCTTATTATTGTCCCCATTGTATTCAGATGGGACGTGTTCGCTCGGATGAAAAACTTTATCATCTTCCTCAGGAGGAT
TTTTTAGCAGCTTCTTTTCTAAACTGGCAGGGAAAACTCACAGCCCCTCAGCAATATATATCTGATACTCTAGTCAAATT
ACATCAGCAACAAAAAACGGTGCTGGTGCAGGCTGTCACGGGTGCAGGAAAAACTGAAATGATTTATCAGATGATTGATA
ACACCCTTAAAAAAGGAAAAGCTGTAGGTTTAACTAGTCCTCGTATTGATGTCTGTCTAGAACTCTACTATCGCTTAAAG
CGAGATTTTTCTTGTCCCATCTCGCTTTTACACGGAAAAAGTGAGAAATACAGCCGCTCGCCACTCGTAATTGCGACGAC
ACATCAGCTTATGCGTTTTCGTCATGCCTTTGACTTAGTCATTCTTGATGAGGTTGATGCTTTTCCATTTCCAGATAATG
ACATGCTCTCTTTTGCACTGACTCAAGCTCGTAAACCTTCTTCAAGCTTGATTTATCTTACTGCAACAACCACCAATAAT
TTGGAAAAACAAGTCAAGTTGGGGCAAATCGAAAAGTTACAACTTCCCCGACGTTTTCATGGTTTTCCATTGGTACTCCC
ACAATTTTTTTGGCAGAGCAAGTTTTATAAAATGGTCAAAAAACAACGCGAATCTGGTTTCCCATTACTTATCTTTGTCC
CTGAAATAAGACTGGGAGAAAAACTGAACCAAGACTTACAGAGTAGTTTTCCTCATGAAGAAATTGCCTTTGTTGCTTCA
ACGAGTCTTGAACGCTTAGAGGCTGTGGAACGTTTTCGCCAGGGCAATATTTCTATCCTTGTCTCAACGACTATTTTAGA
ACGTGGTGTAACTTTTCCTAAGGTTGACGTTTTTGTATTTCAAAGTCATCACCATAATTTCACAAGATCAAGCCTCATCC
AAATTGCTAGACGAGTAGGTCGAAGTACCGAAAGACCTGACGGCAAAGTGTTTTTCTTTCATTTAGGAAAAACAACAGCA
ATGTTGGAAGCTTATAAAAATATCAGAAATATGAATAAGGCAGGAGGTTTCCAATGA

Domains

Predicted by InterproScan.

• Type III restriction enzyme, res subunit (ResIII) (97-236)

• Helicase conserved C-terminal domain (Helicase_C) (274-382)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Lactococcus lactis subsp. cremoris KW2	56.888	93.78	0.533
comFA/cflA	Streptococcus pneumoniae TIGR4	50.585	100	0.517
comFA/cflA	Streptococcus pneumoniae Rx1	50.585	100	0.517
comFA/cflA	Streptococcus pneumoniae D39	50.585	100	0.517
comFA/cflA	Streptococcus pneumoniae R6	50.585	100	0.517
comFA/cflA	Streptococcus mitis SK321	50.117	100	0.512
comFA/cflA	Streptococcus mitis NCTC 12261	49.18	100	0.502
comFA	Bacillus subtilis subsp. subtilis str. 168	37.215	100	0.39
comFA	Latilactobacillus sakei subsp. sakei 23K	38.791	94.976	0.368