Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   OCV52_RS14420 Genome accession   NZ_AP025465
Coordinates   3112186..3113211 (-) Length   341 a.a.
NCBI ID   WP_137406904.1    Uniprot ID   A0A7V7NW93
Organism   Vibrio chagasii strain LMG 21353     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3107186..3118211
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCV52_RS14395 aroK 3108048..3108566 (-) 519 WP_004737165.1 shikimate kinase AroK -
  OCV52_RS14400 pilQ 3108743..3110458 (-) 1716 WP_137406900.1 type IV pilus secretin PilQ Machinery gene
  OCV52_RS14405 pilP 3110494..3111012 (-) 519 WP_137406901.1 pilus assembly protein PilP Machinery gene
  OCV52_RS14410 pilO 3110999..3111610 (-) 612 WP_137406902.1 type 4a pilus biogenesis protein PilO Machinery gene
  OCV52_RS14415 pilN 3111603..3112202 (-) 600 WP_137406903.1 PilN domain-containing protein Machinery gene
  OCV52_RS14420 pilM 3112186..3113211 (-) 1026 WP_137406904.1 type IV pilus assembly protein PilM Machinery gene
  OCV52_RS14425 - 3113363..3115924 (+) 2562 WP_170222424.1 penicillin-binding protein 1A -
  OCV52_RS14430 oxyR 3116018..3116911 (-) 894 WP_004737158.1 DNA-binding transcriptional regulator OxyR -
  OCV52_RS14435 - 3117132..3117860 (+) 729 WP_004737157.1 glutathione peroxidase -

Sequence

Protein


Download         Length: 341 a.a.        Molecular weight: 38156.63 Da        Isoelectric Point: 6.5748

>NTDB_id=91755 OCV52_RS14420 WP_137406904.1 3112186..3113211(-) (pilM) [Vibrio chagasii strain LMG 21353]
MGSSLITGIDINHHSIKAVVLKPVGELYALVGYKELPISDDIFTANHTLEYQKTVKKLKELRKGLPFGCRNVAISVPDNT
VISKVLQIESELEDREKEFSIYQTFAHQSPFPIEELSLDFVKLEDKRFGKGSTSSYQVYATRKEVVDSRAEALTKAGFKP
VVVDTQAHGLLHVWQLASRLYPEKSNWLLVDIGVDQTSLGIIPQGAAPFYKDIAFGTQDLCSIENPNDIEAAFGSAQDTH
QFIVNLIEKLKRQLQLYSSVNVHQPISGIWLMGEGASTPMVAEELERHFQLSCESLNPLSLFENKVARKRRLQMDWQHFG
IAAGMAMSGLKWQGEKHVASN

Nucleotide


Download         Length: 1026 bp        

>NTDB_id=91755 OCV52_RS14420 WP_137406904.1 3112186..3113211(-) (pilM) [Vibrio chagasii strain LMG 21353]
ATGGGTTCATCATTAATTACAGGTATAGATATAAATCACCACAGCATCAAAGCTGTGGTGCTAAAACCCGTGGGGGAATT
GTACGCCCTAGTGGGATACAAAGAGCTGCCGATTTCGGACGACATTTTTACAGCTAACCATACTTTGGAGTATCAGAAAA
CTGTTAAGAAACTCAAAGAACTTAGGAAAGGGCTGCCTTTCGGCTGTCGTAACGTCGCCATTTCGGTACCTGATAACACG
GTGATCAGCAAGGTACTGCAAATTGAGAGTGAACTCGAAGATAGAGAGAAAGAGTTTTCGATCTACCAAACCTTCGCCCA
TCAATCTCCTTTTCCCATCGAGGAGCTGAGTTTAGATTTTGTGAAGCTGGAAGACAAACGATTTGGTAAAGGTTCGACGA
GCAGTTATCAGGTTTATGCTACTCGTAAAGAAGTGGTCGATAGCCGAGCAGAGGCATTAACTAAGGCTGGCTTTAAACCT
GTTGTCGTTGATACGCAGGCGCATGGTTTACTTCATGTTTGGCAGCTTGCCTCACGTTTATACCCAGAGAAAAGTAATTG
GCTGTTGGTGGACATTGGCGTTGATCAAACCTCGCTGGGCATCATTCCTCAGGGAGCAGCCCCTTTCTATAAAGACATCG
CCTTTGGCACTCAAGACCTTTGCAGTATCGAAAACCCCAATGATATCGAGGCTGCGTTTGGCAGTGCTCAGGATACTCAT
CAATTTATCGTTAACTTAATTGAAAAGCTCAAACGTCAGTTGCAGCTCTATTCCTCGGTGAATGTTCACCAACCTATCTC
AGGCATCTGGTTAATGGGCGAGGGCGCCAGCACACCAATGGTGGCTGAAGAGCTTGAACGACATTTTCAGTTGAGCTGTG
AATCATTGAATCCTTTATCCCTGTTTGAGAATAAGGTCGCGAGGAAACGCCGCCTGCAAATGGATTGGCAACATTTTGGT
ATTGCTGCGGGCATGGCGATGAGCGGACTTAAATGGCAGGGAGAGAAGCATGTTGCATCAAATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7V7NW93

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

54.545

100

0.545
  pilM Vibrio campbellii strain DS40M4

49.275

100

0.499

Multiple sequence alignment