Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   U7118_RS09220 Genome accession   NZ_CP141283
Coordinates   1712182..1712799 (+) Length   205 a.a.
NCBI ID   WP_046160608.1    Uniprot ID   -
Organism   Bacillus subtilis strain DKU_09     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1707182..1717799
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U7118_RS09185 (U7118_09185) aroE 1707840..1708682 (+) 843 WP_014480317.1 shikimate dehydrogenase -
  U7118_RS09190 (U7118_09190) yhbY 1708676..1708966 (+) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  U7118_RS09195 (U7118_09195) nadD 1708978..1709547 (+) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  U7118_RS09200 (U7118_09200) yqeK 1709537..1710097 (+) 561 WP_014480316.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  U7118_RS09205 (U7118_09205) rsfS 1710115..1710471 (+) 357 WP_014480315.1 ribosome silencing factor -
  U7118_RS09210 (U7118_09210) yqeM 1710468..1711211 (+) 744 WP_069837641.1 class I SAM-dependent DNA methyltransferase -
  U7118_RS09215 (U7118_09215) comER 1711277..1712098 (-) 822 WP_032726195.1 late competence protein ComER -
  U7118_RS09220 (U7118_09220) comEA 1712182..1712799 (+) 618 WP_046160608.1 competence protein ComEA Machinery gene
  U7118_RS09225 (U7118_09225) comEB 1712866..1713435 (+) 570 WP_003229978.1 ComE operon protein 2 -
  U7118_RS09230 (U7118_09230) comEC 1713439..1715769 (+) 2331 WP_069837640.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  U7118_RS09235 (U7118_09235) yqzM 1715809..1715943 (-) 135 WP_003229983.1 YqzM family protein -
  U7118_RS09240 (U7118_09240) - 1715984..1716133 (+) 150 WP_003229985.1 hypothetical protein -
  U7118_RS09245 (U7118_09245) holA 1716173..1717216 (+) 1044 WP_029317888.1 DNA polymerase III subunit delta -
  U7118_RS09250 (U7118_09250) rpsT 1717231..1717497 (-) 267 WP_003229989.1 30S ribosomal protein S20 -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21738.48 Da        Isoelectric Point: 4.7284

>NTDB_id=916813 U7118_RS09220 WP_046160608.1 1712182..1712799(+) (comEA) [Bacillus subtilis strain DKU_09]
MNWLNQHKKAIVLAASAAVFTAIMIFLATGKNKEPVKQAVPTEAENTVVKQEANNDESSETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEEITKVSGIGEKLFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=916813 U7118_RS09220 WP_046160608.1 1712182..1712799(+) (comEA) [Bacillus subtilis strain DKU_09]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTGTTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGGCAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAGCGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGAGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTTTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGAAATCACTAAGGT
TTCAGGAATAGGTGAAAAGTTATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(63-118)

Helix-hairpin-helix motif (HHH_3)

(142-203)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

97.561

100

0.976
  comEA Staphylococcus aureus MW2

35.909

100

0.385
  comEA Staphylococcus aureus N315

35

100

0.376