Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   U7118_RS03030 Genome accession   NZ_CP141283
Coordinates   575403..576794 (+) Length   463 a.a.
NCBI ID   WP_014481052.1    Uniprot ID   -
Organism   Bacillus subtilis strain DKU_09     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 570403..581794
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U7118_RS03005 (U7118_03005) - 570562..571554 (-) 993 WP_014481056.1 LCP family protein -
  U7118_RS03010 (U7118_03010) - 571554..572207 (-) 654 WP_014481055.1 YigZ family protein -
  U7118_RS03015 (U7118_03015) degS 572424..573581 (+) 1158 WP_003227983.1 two-component sensor histidine kinase DegS Regulator
  U7118_RS03020 (U7118_03020) degU 573664..574353 (+) 690 WP_014481054.1 two-component system response regulator DegU Regulator
  U7118_RS03025 (U7118_03025) fakBA 574451..575296 (+) 846 WP_014481053.1 DegV family protein -
  U7118_RS03030 (U7118_03030) comFA 575403..576794 (+) 1392 WP_014481052.1 ATP-dependent helicase ComFA Machinery gene
  U7118_RS03035 (U7118_03035) comFB 576854..577150 (+) 297 WP_003227989.1 late competence protein ComFB -
  U7118_RS03040 (U7118_03040) comFC 577114..577836 (+) 723 WP_014481051.1 comF operon protein ComFC Machinery gene
  U7118_RS03045 (U7118_03045) yvyF 577909..578328 (+) 420 WP_038429728.1 TIGR03826 family flagellar region protein -
  U7118_RS03050 (U7118_03050) flgM 578409..578675 (+) 267 WP_014481049.1 flagellar biosynthesis anti-sigma factor FlgM -
  U7118_RS03055 (U7118_03055) flgN 578691..579173 (+) 483 WP_014481048.1 flagellar protein FlgN -
  U7118_RS03060 (U7118_03060) flgK 579192..580715 (+) 1524 WP_324271437.1 flagellar hook-associated protein FlgK -
  U7118_RS03065 (U7118_03065) flgL 580726..581622 (+) 897 WP_014481046.1 flagellar hook-associated protein FlgL -

Sequence

Protein


Download         Length: 463 a.a.        Molecular weight: 52543.49 Da        Isoelectric Point: 9.7976

>NTDB_id=916796 U7118_RS03030 WP_014481052.1 575403..576794(+) (comFA) [Bacillus subtilis strain DKU_09]
MNVPVEKNSSFSRELQQTLRSRHLLRTELSFSDEMIEWHIKNGYITAENSISINKRGYRCNRCGQTDQRYYSFYHSSGKN
KLYCRSCVMMGRVSEEVPLYSWEEENEPNWQSIKLTWDGKLSSGQQKAANVLIEAISKKEELLIWAVCGAGKTEMLFPGI
ESALNQGLRVCIATPRTDVVLELAPRLKAAFQGADISALYGGSDDKGRLSPLMISTTHQLLRYKDAIDVMIIDEVDAFPY
SADQTLQFAVQKARKKNSTLVYLSATPPKELKRKALNGQLHSVRIPARHHRKPLPEPRFVWCGNWKKKLNRNKIPPAVKR
WIEFHVKEGRPVFLFVPSVSILEKAAACFRGVHCRTASVHAEDKHRKEKVQQFRDGQLDLLITTTILERGVTVPKVQTGV
LGAESPIFTESALVQIAGRTGRHKEYADGDVIFFHFGKTKSMLDARKHIKEMNELAAKVECTD

Nucleotide


Download         Length: 1392 bp        

>NTDB_id=916796 U7118_RS03030 WP_014481052.1 575403..576794(+) (comFA) [Bacillus subtilis strain DKU_09]
GTGAATGTGCCAGTTGAAAAAAACAGTTCCTTTTCTAGAGAATTGCAGCAGACGCTTCGAAGCCGTCATTTGCTCAGGAC
TGAGCTCTCATTTTCCGATGAGATGATTGAATGGCATATCAAGAATGGATATATCACTGCTGAAAATTCTATATCCATAA
ATAAACGGGGATATAGATGTAATAGGTGCGGCCAAACTGATCAGCGGTATTATTCTTTTTATCACTCATCTGGAAAGAAT
AAGCTGTATTGCCGTTCCTGTGTCATGATGGGCAGAGTGAGTGAGGAGGTTCCTTTATATTCATGGGAAGAGGAAAATGA
ACCAAACTGGCAGTCAATTAAACTGACATGGGACGGCAAGCTTTCAAGCGGACAGCAAAAAGCCGCCAATGTTTTAATTG
AAGCAATATCAAAAAAAGAAGAGCTCCTCATCTGGGCGGTTTGCGGCGCTGGCAAAACAGAAATGCTGTTTCCTGGTATA
GAATCAGCGTTAAATCAAGGACTGCGTGTATGTATTGCAACACCTCGCACCGATGTTGTATTAGAGCTTGCTCCAAGGCT
CAAGGCTGCCTTTCAGGGGGCTGACATTTCAGCGCTTTACGGAGGAAGCGATGACAAAGGGCGGCTATCTCCGCTTATGA
TTTCCACTACGCATCAGCTTTTGCGATATAAAGATGCAATCGATGTTATGATCATTGATGAAGTTGACGCTTTTCCATAT
TCCGCTGATCAAACCCTTCAATTCGCTGTTCAAAAAGCAAGAAAGAAAAACAGCACCCTCGTTTATTTAAGTGCAACACC
TCCTAAAGAATTAAAAAGAAAAGCACTGAACGGACAGTTACATTCAGTTCGCATCCCCGCAAGACACCACCGGAAACCTT
TACCCGAACCGCGCTTTGTATGGTGTGGAAACTGGAAGAAGAAATTAAACCGAAATAAAATTCCGCCAGCGGTGAAAAGA
TGGATAGAGTTTCATGTAAAAGAAGGGAGGCCTGTTTTTTTATTCGTTCCTTCCGTTTCTATTCTGGAAAAGGCTGCTGC
GTGCTTTAGAGGTGTTCATTGCCGAACCGCATCTGTGCACGCGGAAGACAAGCATAGAAAGGAGAAAGTGCAGCAATTCA
GAGATGGTCAGCTCGATCTATTAATCACAACAACAATACTGGAAAGAGGCGTCACAGTCCCCAAGGTGCAAACGGGTGTA
CTAGGAGCGGAATCACCTATCTTTACGGAAAGCGCACTTGTTCAAATTGCAGGAAGAACCGGCCGGCATAAAGAATATGC
GGACGGCGATGTCATTTTCTTTCACTTCGGCAAAACAAAGAGTATGCTCGATGCAAGAAAGCATATAAAAGAAATGAATG
AATTGGCAGCAAAAGTTGAATGTACAGACTAG

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(120-268)

Helicase conserved C-terminal domain (Helicase_C)

(326-423)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

98.056

100

0.981