NTDB

Overview

Name	comEA	Type	Machinery gene
Locus tag	U2H22_RS13615	Genome accession	NZ_CP141265
Coordinates	2716853..2717467 (-)	Length	204 a.a.
NCBI ID	WP_015240245.1	Uniprot ID	-
Organism	Bacillus velezensis strain L2D39
Function	dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2711853..2722467

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
U2H22_RS13585 (U2H22_13585)	rpsT	2712156..2712422 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
U2H22_RS13590 (U2H22_13590)	holA	2712439..2713480 (-)	1042	Protein_2633	DNA polymerase III subunit delta	-
U2H22_RS13595 (U2H22_13595)	-	2713520..2713671 (-)	152	Protein_2634	hypothetical protein	-
U2H22_RS13600 (U2H22_13600)	-	2713712..2713846 (+)	135	WP_003152870.1	YqzM family protein	-
U2H22_RS13605 (U2H22_13605)	comEC	2713865..2716216 (-)	2352	WP_015240244.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
U2H22_RS13610 (U2H22_13610)	-	2716217..2716786 (-)	570	WP_003152868.1	ComE operon protein 2	-
U2H22_RS13615 (U2H22_13615)	comEA	2716853..2717467 (-)	615	WP_015240245.1	helix-hairpin-helix domain-containing protein	Machinery gene
U2H22_RS13620 (U2H22_13620)	comER	2717526..2718347 (+)	822	WP_015240246.1	late competence protein ComER	-
U2H22_RS13625 (U2H22_13625)	-	2718416..2719153 (-)	738	WP_015240247.1	class I SAM-dependent methyltransferase	-
U2H22_RS13630 (U2H22_13630)	rsfS	2719150..2719506 (-)	357	WP_007408260.1	ribosome silencing factor	-
U2H22_RS13635 (U2H22_13635)	yqeK	2719524..2720084 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
U2H22_RS13640 (U2H22_13640)	-	2720074..2720643 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
U2H22_RS13645 (U2H22_13645)	yhbY	2720654..2720944 (-)	291	WP_007408257.1	ribosome assembly RNA-binding protein YhbY	-
U2H22_RS13650 (U2H22_13650)	aroE	2720938..2721780 (-)	843	WP_015240248.1	shikimate dehydrogenase	-

Sequence

Protein

Download Length: 204 a.a. Molecular weight: 22209.32 Da Isoelectric Point: 5.5211

                                >NTDB_id=916675 U2H22_RS13615 WP_015240245.1 2716853..2717467(-) (comEA) [Bacillus velezensis strain L2D39]

MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY

EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG

QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

Download Length: 615 bp

                                >NTDB_id=916675 U2H22_RS13615 WP_015240245.1 2716853..2717467(-) (comEA) [Bacillus velezensis strain L2D39]

ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT

TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCTGTGCTCGGCCAAACAGCCTCAGCAT

CCGTCGCGGACAAAAAAGACACAGCAGACGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGGAATCCCGGCGTGTAT

GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT

TAATTTAGCAGAGCAGCTGCAGGACGGCACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG

CGGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCAATTTCCGGGGTGGGG

CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC

AGGCTTTGGAGAGAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363

Detailed information NTG0916675