Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   U6037_RS01970 Genome accession   NZ_CP140706
Coordinates   441152..442216 (+) Length   354 a.a.
NCBI ID   WP_322845619.1    Uniprot ID   -
Organism   Pseudomonas sp. B33.4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 436152..447216
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U6037_RS01960 - 436905..438173 (+) 1269 WP_090280412.1 malic enzyme-like NAD(P)-binding protein -
  U6037_RS01965 - 438488..440932 (-) 2445 WP_416221715.1 penicillin-binding protein 1A -
  U6037_RS01970 pilM 441152..442216 (+) 1065 WP_322845619.1 pilus assembly protein PilM Machinery gene
  U6037_RS01975 - 442216..442782 (+) 567 WP_322845620.1 PilN domain-containing protein -
  U6037_RS01980 pilO 442779..443402 (+) 624 WP_127925528.1 type 4a pilus biogenesis protein PilO -
  U6037_RS01985 - 443399..443926 (+) 528 WP_150776183.1 pilus assembly protein PilP -
  U6037_RS01990 pilQ 443940..446018 (+) 2079 WP_322845621.1 type IV pilus secretin PilQ Machinery gene
  U6037_RS01995 aroK 446023..446541 (+) 519 WP_011332081.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38122.74 Da        Isoelectric Point: 4.9006

>NTDB_id=915028 U6037_RS01970 WP_322845619.1 441152..442216(+) (pilM) [Pseudomonas sp. B33.4]
MLGLFNKKTNTLLGIDISSTSVKLLELSRQGDRYRVEAYAVEPLPANAVVEKNIAELEGVGQALSRVLVKARTGLKGVAV
AVAGSAVITKIIEMDAGLSDDELENQLKIEADQYIPYPLDEVAIDFEVQGVSPRNPERVNVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLATQLAASQERLTVAVVDIGATMTTLSVLHNGKIIYTREQLFGGRQLTEEIQRRYGLTI
EQAGLAKKQGGLPDDYISEVLQPFREALVQQVSRSLQFFFASGQYNAVDHILLAGGTASVPGLDRLIEQRLKTPTQVANP
FSNMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=915028 U6037_RS01970 WP_322845619.1 441152..442216(+) (pilM) [Pseudomonas sp. B33.4]
GTGCTGGGACTCTTCAATAAAAAGACCAATACGTTACTGGGGATCGACATCAGCTCCACCTCGGTGAAGCTGCTGGAACT
GAGCCGTCAGGGTGATCGCTACCGGGTCGAGGCGTATGCGGTCGAGCCATTGCCGGCCAACGCCGTGGTCGAGAAAAACA
TCGCCGAACTCGAAGGCGTTGGCCAGGCCCTGAGCCGGGTGCTGGTCAAGGCGCGCACCGGGCTCAAGGGCGTGGCAGTT
GCAGTGGCCGGTTCGGCGGTGATTACCAAAATCATCGAAATGGACGCCGGACTTTCCGACGACGAACTGGAAAACCAGCT
CAAGATCGAAGCTGATCAATACATCCCTTATCCGCTGGACGAAGTCGCCATCGACTTCGAAGTCCAGGGTGTTTCGCCGC
GTAACCCAGAACGGGTCAACGTGCTGCTGGCCGCCTGTCGCAAGGAAAACGTCGAAGTCCGTGAAGCGGCGCTGGCACTC
GCCGGGCTGACCGCGCGGGTCGTCGATGTCGAAGCCTACGCGCTGGAACGATCTTTCGGGCTGCTCGCCACGCAACTGGC
CGCCTCGCAGGAACGCCTGACGGTTGCGGTGGTCGACATCGGCGCGACCATGACCACCCTCAGCGTTCTGCACAACGGCA
AGATCATCTACACCCGCGAGCAATTGTTCGGTGGTCGGCAACTGACTGAGGAAATCCAGCGTCGTTATGGCCTGACCATC
GAGCAGGCGGGGCTGGCGAAAAAGCAGGGCGGTCTGCCCGATGATTACATCAGCGAGGTCTTGCAGCCGTTTCGTGAAGC
GCTGGTACAGCAAGTGTCGCGCTCGTTGCAGTTTTTCTTCGCCTCCGGGCAATACAACGCGGTCGACCACATTCTGCTGG
CCGGCGGTACGGCCTCAGTGCCGGGGCTGGACCGGCTGATCGAGCAGCGCCTGAAGACGCCGACGCAAGTGGCCAATCCG
TTTTCCAATATGGCCTTGAGCAGCAAGGTCAATGCCGGAGCGCTGGCCAGCGACGCGCCGGCCCTGATGATCGCCTGCGG
GCTCGCGCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.497

100

0.565
  comM Acinetobacter nosocomialis M2

56.497

100

0.565
  comM Acinetobacter baylyi ADP1

55.085

100

0.551
  pilM Legionella pneumophila strain ERS1305867

47.458

100

0.475