Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   U6037_RS01990 Genome accession   NZ_CP140706
Coordinates   443940..446018 (+) Length   692 a.a.
NCBI ID   WP_322845621.1    Uniprot ID   -
Organism   Pseudomonas sp. B33.4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 438940..451018
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U6037_RS01970 pilM 441152..442216 (+) 1065 WP_322845619.1 pilus assembly protein PilM Machinery gene
  U6037_RS01975 - 442216..442782 (+) 567 WP_322845620.1 PilN domain-containing protein -
  U6037_RS01980 pilO 442779..443402 (+) 624 WP_127925528.1 type 4a pilus biogenesis protein PilO -
  U6037_RS01985 - 443399..443926 (+) 528 WP_150776183.1 pilus assembly protein PilP -
  U6037_RS01990 pilQ 443940..446018 (+) 2079 WP_322845621.1 type IV pilus secretin PilQ Machinery gene
  U6037_RS01995 aroK 446023..446541 (+) 519 WP_011332081.1 shikimate kinase AroK -
  U6037_RS02000 aroB 446694..447794 (+) 1101 WP_322845622.1 3-dehydroquinate synthase -
  U6037_RS02005 - 447805..449397 (+) 1593 WP_322845623.1 AAA family ATPase -

Sequence

Protein


Download         Length: 692 a.a.        Molecular weight: 74919.44 Da        Isoelectric Point: 5.8240

>NTDB_id=915029 U6037_RS01990 WP_322845621.1 443940..446018(+) (pilQ) [Pseudomonas sp. B33.4]
MNRIFSTLGFSLWIALMSPMVLAANLKTLDVAALPGDRVELKLSFDGPPPQPKGYTTESPARIALDLPGVVSQLASKNLD
LGSGNARTATVVEAKDRTRLIVSLTQLAPYNTRVAGNNLFVVVGQGAPAAAPRAAAVTPRAAVAATAPAKAFVPKSRAIR
GVDFQRGTAGEGNVVIDLSDPTIAPDIQEHDGKIILSFARTQLPEKLRVRLDVKDFATPVQFVNAAVSGDRTVISVEPGG
TYEYSTFQTDNKLTVSIRPMTVDDLQKRNADRQAYVGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQN
VPWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVTSAEA
KIDERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNKGNWNASGVN
GSSSTIGTPGSTSTNSPFVDMGTVNNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPY
QEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFSN
TQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2079 bp        

>NTDB_id=915029 U6037_RS01990 WP_322845621.1 443940..446018(+) (pilQ) [Pseudomonas sp. B33.4]
ATGAACAGGATTTTCTCCACCCTCGGTTTTTCGCTATGGATAGCGCTGATGTCGCCGATGGTACTCGCGGCCAATCTGAA
GACGCTGGATGTGGCGGCGTTGCCGGGTGATCGCGTCGAGCTGAAGCTGTCGTTCGACGGCCCACCGCCACAGCCCAAGG
GCTACACCACCGAATCACCGGCACGCATCGCCCTCGATTTGCCCGGTGTCGTCAGTCAATTGGCCAGCAAGAACCTTGAT
CTGGGCAGCGGCAATGCACGCACCGCCACGGTAGTCGAAGCCAAGGACCGCACGCGGCTGATCGTCAGTCTGACGCAACT
GGCGCCTTACAACACGCGGGTCGCGGGCAACAATCTGTTCGTGGTGGTTGGGCAGGGCGCGCCGGCGGCGGCGCCGAGAG
CTGCAGCCGTTACACCACGCGCTGCGGTAGCGGCTACGGCACCGGCCAAGGCCTTTGTGCCGAAAAGCCGGGCCATTCGC
GGCGTGGATTTCCAGCGGGGTACGGCAGGTGAGGGCAACGTGGTCATCGATCTCTCCGACCCGACCATCGCTCCGGATAT
CCAGGAGCATGACGGCAAGATCATCCTCAGCTTTGCCCGCACACAACTCCCGGAAAAGCTACGGGTACGCCTCGACGTCA
AGGATTTCGCCACCCCGGTGCAGTTCGTCAACGCGGCTGTGAGCGGTGATCGCACGGTCATCAGCGTCGAACCCGGCGGG
ACTTACGAGTACTCCACCTTTCAGACCGACAACAAGCTGACGGTCAGCATCCGTCCGATGACCGTCGATGATCTGCAAAA
GCGTAACGCTGACCGTCAGGCGTACGTCGGCGAAAAACTCTCGCTGAATTTTCAGGACATTGATGTGCGTTCAGTGCTGC
AACTGATCGCCGACTTCACCAACCTCAACCTTGTCGCCAGCGACACGGTGCAAGGTGGTATCACCTTGCGCCTGCAGAAC
GTGCCGTGGGATCAGGCGCTGGATCTGGTGCTGAAAACCAAGGGCCTGGATAAACGCAAGATCGGCAATGTGCTGCTGGT
TGCTCCGGCCGATGAAATCGCTGCCCGCGAGCGCCAGGAACTGGAGTCGCAGAAGCAGATTGCCGAACTGGCACCACTGC
GTCGCGAACTTTTGCAGGTGAACTACGCCAAGGCAGCGGACATCGCCAAGCTGTTCCAGTCGGTGACCAGTGCCGAGGCT
AAAATCGACGAGCGTGGCTCGATCACCGTCGATGAGCGGACCAACAACATCATTGCCTATCAGACTCAGGATCGTCTCGA
CGAACTGCGGCGGATCGTGGCACAGCTGGATATACCGGTGCGTCAGGTGATGATCGAGGCGCGGATCGTCGAGGCTAACG
TCGATTACGACAAAAGCCTGGGCGTGCGCTGGGGCGGTTCGATCCAGAACAAGGGCAATTGGAACGCCTCCGGGGTCAAC
GGTTCGTCGAGCACCATTGGTACTCCTGGCAGCACCAGTACCAACTCGCCGTTCGTCGACATGGGCACCGTCAACAATAC
TTCAGGGATCGGCATCGCGTTCATCACCGACAACGTCTTGCTCGATCTTGAGCTGACGGCCATGGAGAAAACCGGCAACG
GTGAAATCGTTTCGCAGCCCAAGGTCGTCACCTCGGACAAGGAAACCGCGAAAATCCTCAAAGGCACCGAGATTCCGTAT
CAGGAAGCCAGCTCCAGCGGCGCCACGTCGGTGTCGTTCAAGGAGGCCTCGCTGTCGCTGGAAGTCACGCCGCAGATCAC
CCCCGACAATCGCATCATCATGGAGGTCAAGGTCACCAAGGACGAACCGGATTACCTGAACAAAGTGCAGGATGTACCGC
CGATCAAGAAAAACGAGGTCAACGCCAAGGTGCTGGTCAACGACGGCGAAACCATCGTGATTGGGGGCGTTTTCTCAAAT
ACTCAAAGCAAGGTCGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGTGGT
TTCGGAGAAAAAATCCGAGCTGCTGGTATTTCTCACACCACGTATCATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(37-121)

Bacterial type II and III secretion system protein (Secretin)

(527-683)

Secretin and TonB N terminus short domain (STN)

(303-349)

Bacterial type II/III secretion system short domain (Secretin_N)

(377-442)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.486

100

0.75
  comQ Acinetobacter baylyi ADP1

41.643

100

0.418
  pilQ Acinetobacter baumannii strain A118

37.652

100

0.403
  pilQ Acinetobacter baumannii D1279779

37.652

100

0.403
  pilQ Legionella pneumophila strain Lp02

38.053

97.977

0.373
  pilQ Legionella pneumophila strain ERS1305867

38.018

97.688

0.371