Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   L6430_RS20860 Genome accession   NZ_AP025342
Coordinates   4141447..4142076 (-) Length   209 a.a.
NCBI ID   WP_059231264.1    Uniprot ID   A0AAW6KB53
Organism   Bacillus paralicheniformis strain J41TS8     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4136447..4147076
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L6430_RS20840 holA 4137006..4138031 (-) 1026 WP_165395388.1 DNA polymerase III subunit delta -
  L6430_RS20845 - 4138261..4138392 (+) 132 WP_003183686.1 YqzM family protein -
  L6430_RS20850 - 4138417..4140804 (-) 2388 WP_059231267.1 DNA internalization-related competence protein ComEC/Rec2 -
  L6430_RS20855 - 4140809..4141378 (-) 570 WP_020452273.1 ComE operon protein 2 -
  L6430_RS20860 comEA 4141447..4142076 (-) 630 WP_059231264.1 helix-hairpin-helix domain-containing protein Machinery gene
  L6430_RS20865 comER 4142159..4142980 (+) 822 WP_059231262.1 late competence protein ComER -
  L6430_RS20870 - 4143051..4143806 (-) 756 WP_059231261.1 class I SAM-dependent DNA methyltransferase -
  L6430_RS20875 rsfS 4143803..4144159 (-) 357 WP_059231259.1 ribosome silencing factor -
  L6430_RS20880 yqeK 4144173..4144736 (-) 564 WP_059231257.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  L6430_RS20885 - 4144726..4145295 (-) 570 WP_059231254.1 nicotinate-nucleotide adenylyltransferase -
  L6430_RS20890 yhbY 4145314..4145604 (-) 291 WP_003183704.1 ribosome assembly RNA-binding protein YhbY -
  L6430_RS20895 aroE 4145598..4146434 (-) 837 WP_059231252.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 209 a.a.        Molecular weight: 22498.33 Da        Isoelectric Point: 4.7849

>NTDB_id=91497 L6430_RS20860 WP_059231264.1 4141447..4142076(-) (comEA) [Bacillus paralicheniformis strain J41TS8]
MTDWLKQYKWHAAGGVALVLIISAAFMLLSGKRETSSGFSIPEEASAQTFDKKEEVKREKNAGDEAVIIDLKGAVKNPGV
YQMKEGDRVHDALKKAGGTEKKADQKQINLAAVLRDGMVLYIPFEGEEAAGSLSEADSRADGSSSDIVNINTASSAELQT
IPGIGPSKAEAVVEYREENGMFQTIEDITNVSGIGEKSFERIKSSITVK

Nucleotide


Download         Length: 630 bp        

>NTDB_id=91497 L6430_RS20860 WP_059231264.1 4141447..4142076(-) (comEA) [Bacillus paralicheniformis strain J41TS8]
TTGACAGATTGGCTAAAACAATATAAATGGCATGCGGCGGGAGGTGTGGCACTCGTCTTGATCATCAGCGCTGCCTTCAT
GCTGTTAAGCGGAAAGCGTGAAACTTCATCCGGCTTCTCCATTCCTGAAGAGGCTTCTGCACAGACTTTTGACAAGAAAG
AGGAGGTGAAGCGGGAAAAAAACGCGGGGGATGAGGCGGTTATCATCGATTTGAAAGGCGCTGTGAAAAATCCGGGCGTC
TATCAAATGAAGGAGGGAGACAGGGTGCACGATGCATTAAAAAAAGCAGGCGGCACCGAGAAAAAAGCGGACCAAAAGCA
GATTAATCTGGCAGCCGTTTTGCGGGACGGTATGGTTCTTTACATTCCATTTGAAGGGGAAGAGGCCGCTGGCTCTCTTT
CGGAAGCGGACTCAAGAGCAGACGGCAGCTCAAGTGATATCGTCAATATCAATACGGCTTCCTCTGCGGAGCTTCAGACG
ATTCCCGGGATCGGTCCTTCAAAAGCGGAAGCGGTAGTCGAATACCGCGAGGAGAACGGAATGTTTCAGACGATTGAAGA
CATAACAAACGTTTCGGGAATTGGAGAAAAGTCTTTTGAAAGAATAAAATCTTCCATCACGGTAAAGTAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(145-207)

SLBB domain (SLBB)

(69-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

51.869

100

0.531
  comEA Staphylococcus aureus MW2

36.364

100

0.383
  comEA Staphylococcus aureus N315

35.909

100

0.378

Multiple sequence alignment