Detailed information    

insolico Bioinformatically predicted

Overview


Name   comGC   Type   Machinery gene
Locus tag   L6430_RS20300 Genome accession   NZ_AP025342
Coordinates   4040698..4040991 (-) Length   97 a.a.
NCBI ID   WP_020452176.1    Uniprot ID   A0A6N2FHJ1
Organism   Bacillus paralicheniformis strain J41TS8     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 4035698..4045991
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L6430_RS20255 tasA 4036075..4036869 (-) 795 WP_020452167.1 biofilm matrix protein TasA -
  L6430_RS20260 sipW 4036942..4037526 (-) 585 WP_065644169.1 signal peptidase I SipW -
  L6430_RS20265 tapA 4037523..4038251 (-) 729 WP_020452169.1 amyloid fiber anchoring/assembly protein TapA -
  L6430_RS20270 - 4038529..4038849 (+) 321 WP_020452170.1 YqzG/YhdC family protein -
  L6430_RS20275 - 4038879..4039061 (-) 183 WP_020452171.1 YqzE family protein -
  L6430_RS20280 comGG 4039150..4039515 (-) 366 WP_020452172.1 competence type IV pilus minor pilin ComGG -
  L6430_RS20285 comGF 4039527..4040015 (-) 489 WP_236613657.1 competence type IV pilus minor pilin ComGF -
  L6430_RS20290 comGE 4039924..4040271 (-) 348 WP_020452174.1 competence type IV pilus minor pilin ComGE -
  L6430_RS20295 comGD 4040255..4040692 (-) 438 WP_020452175.1 competence type IV pilus minor pilin ComGD -
  L6430_RS20300 comGC 4040698..4040991 (-) 294 WP_020452176.1 competence type IV pilus major pilin ComGC Machinery gene
  L6430_RS20305 comGB 4041005..4042039 (-) 1035 WP_041817253.1 competence type IV pilus assembly protein ComGB Machinery gene
  L6430_RS20310 comGA 4042029..4043093 (-) 1065 WP_020452178.1 competence type IV pilus ATPase ComGA Machinery gene
  L6430_RS20315 - 4043251..4044090 (-) 840 WP_020452179.1 STAS domain-containing protein -
  L6430_RS20325 - 4044332..4044709 (-) 378 WP_003183482.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  L6430_RS20330 - 4044920..4045165 (+) 246 WP_020452180.1 DUF2626 domain-containing protein -
  L6430_RS20335 - 4045201..4045839 (-) 639 WP_020452181.1 MBL fold metallo-hydrolase -

Sequence


Protein


Download         Length: 97 a.a.        Molecular weight: 10670.54 Da        Isoelectric Point: 9.1200

>NTDB_id=91492 L6430_RS20300 WP_020452176.1 4040698..4040991(-) (comGC) [Bacillus paralicheniformis strain J41TS8]
MNEKGFTLIEMLVVMLVISILLLITIPNVTKHNQSIQKKGCEGLINMVQAQITAYQMDHDGKTPSRADLESEGYVKKNLK
CPNGKAIKISNGKAQAD

Nucleotide


Download         Length: 294 bp        

>NTDB_id=91492 L6430_RS20300 WP_020452176.1 4040698..4040991(-) (comGC) [Bacillus paralicheniformis strain J41TS8]
GTGAATGAAAAGGGATTTACACTGATAGAAATGTTAGTCGTCATGCTCGTCATCTCCATCCTGCTGTTGATCACCATACC
GAATGTGACCAAGCATAATCAAAGTATTCAAAAGAAAGGATGCGAAGGGTTAATTAATATGGTTCAAGCGCAAATCACGG
CTTATCAAATGGATCATGACGGGAAAACGCCGAGTAGGGCGGACCTTGAATCTGAAGGTTACGTGAAGAAGAACTTAAAG
TGCCCGAATGGGAAAGCAATTAAAATTTCAAACGGCAAAGCACAGGCTGATTGA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A6N2FHJ1

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC Bacillus subtilis subsp. subtilis str. 168

71.134

100

0.711

  comGC Staphylococcus aureus MW2

45.263

97.938

0.443

  comGC Staphylococcus aureus N315

45.263

97.938

0.443


Multiple sequence alignment