Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   L6430_RS20305 Genome accession   NZ_AP025342
Coordinates   4041005..4042039 (-) Length   344 a.a.
NCBI ID   WP_041817253.1    Uniprot ID   -
Organism   Bacillus paralicheniformis strain J41TS8     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4036005..4047039
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L6430_RS20255 tasA 4036075..4036869 (-) 795 WP_020452167.1 biofilm matrix protein TasA -
  L6430_RS20260 sipW 4036942..4037526 (-) 585 WP_065644169.1 signal peptidase I SipW -
  L6430_RS20265 tapA 4037523..4038251 (-) 729 WP_020452169.1 amyloid fiber anchoring/assembly protein TapA -
  L6430_RS20270 - 4038529..4038849 (+) 321 WP_020452170.1 YqzG/YhdC family protein -
  L6430_RS20275 - 4038879..4039061 (-) 183 WP_020452171.1 YqzE family protein -
  L6430_RS20280 comGG 4039150..4039515 (-) 366 WP_020452172.1 competence type IV pilus minor pilin ComGG -
  L6430_RS20285 comGF 4039527..4040015 (-) 489 WP_236613657.1 competence type IV pilus minor pilin ComGF -
  L6430_RS20290 comGE 4039924..4040271 (-) 348 WP_020452174.1 competence type IV pilus minor pilin ComGE -
  L6430_RS20295 comGD 4040255..4040692 (-) 438 WP_020452175.1 competence type IV pilus minor pilin ComGD -
  L6430_RS20300 comGC 4040698..4040991 (-) 294 WP_020452176.1 competence type IV pilus major pilin ComGC Machinery gene
  L6430_RS20305 comGB 4041005..4042039 (-) 1035 WP_041817253.1 competence type IV pilus assembly protein ComGB Machinery gene
  L6430_RS20310 comGA 4042029..4043093 (-) 1065 WP_020452178.1 competence type IV pilus ATPase ComGA Machinery gene
  L6430_RS20315 - 4043251..4044090 (-) 840 WP_020452179.1 STAS domain-containing protein -
  L6430_RS20325 - 4044332..4044709 (-) 378 WP_003183482.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  L6430_RS20330 - 4044920..4045165 (+) 246 WP_020452180.1 DUF2626 domain-containing protein -
  L6430_RS20335 - 4045201..4045839 (-) 639 WP_020452181.1 MBL fold metallo-hydrolase -
  L6430_RS20340 - 4045995..4046168 (+) 174 WP_003183486.1 DUF2759 domain-containing protein -
  L6430_RS20345 - 4046229..4046543 (-) 315 WP_020452182.1 MTH1187 family thiamine-binding protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 39780.12 Da        Isoelectric Point: 9.7341

>NTDB_id=91493 L6430_RS20305 WP_041817253.1 4041005..4042039(-) (comGB) [Bacillus paralicheniformis strain J41TS8]
MKIKKKWPVREQADILKKLGDMMKSGYTILDALSMLKLQLNERRRADLTFAMEKLTEGRPVFQVLEMISFHKDAVTIVYF
AEQHGNLPYAFRQSGELLHRKIAQTEKLKKAARYPLFLVLTVCVIISIMKSAIIPQFSAIYESMNVETSFATTLIFSVFD
HFYLFIAGMMLIAAALSLYYLCSFRHKPPEDKMTFLIRIPLLGQTFKLFNSYFLSLQLSNLLQAGLSVYDSLKAFESQPF
LSFHKNEAKRLIERLKQGESLEQMLAGHPFYENDLAKAVAHGQLNGLLYRELYSYSQFLIERLERKAEKWTGLIQPLIYG
LTAAMILILYLSMLLPMYQMMNQL

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=91493 L6430_RS20305 WP_041817253.1 4041005..4042039(-) (comGB) [Bacillus paralicheniformis strain J41TS8]
ATGAAGATTAAAAAGAAGTGGCCCGTCCGGGAACAGGCGGACATTCTGAAAAAACTCGGAGACATGATGAAGAGCGGATA
TACGATTTTGGATGCTTTAAGTATGCTGAAACTGCAATTGAATGAAAGGCGAAGAGCAGATCTGACATTTGCGATGGAGA
AGCTGACAGAAGGGCGACCTGTGTTTCAAGTGCTTGAAATGATTTCATTTCATAAGGATGCTGTTACCATTGTTTACTTT
GCCGAACAGCACGGGAATTTGCCATATGCCTTTCGGCAGAGCGGAGAGCTGCTTCACCGCAAGATCGCTCAAACCGAAAA
ACTAAAGAAAGCCGCCAGATATCCCTTGTTTTTAGTTTTAACGGTCTGCGTCATTATCTCTATTATGAAATCGGCAATCA
TCCCGCAATTTTCCGCGATCTATGAATCAATGAACGTTGAAACGTCTTTTGCAACGACCCTCATCTTTTCGGTTTTTGAT
CATTTTTATCTTTTTATCGCAGGAATGATGTTGATTGCCGCCGCTTTATCCCTCTATTATCTGTGTTCATTTCGCCATAA
ACCTCCAGAAGACAAAATGACCTTTCTCATTAGAATTCCCTTGCTGGGCCAAACCTTCAAACTATTCAACAGTTATTTTT
TATCGCTCCAATTGAGCAATCTTTTGCAAGCTGGTCTGTCTGTATATGACAGTTTAAAAGCATTTGAAAGCCAGCCGTTT
TTATCATTTCATAAAAATGAAGCGAAGCGTCTGATCGAGCGGCTGAAACAAGGGGAGAGTCTGGAGCAGATGCTGGCCGG
GCATCCGTTTTATGAAAATGATCTGGCGAAGGCTGTTGCTCACGGCCAATTAAACGGACTCTTATACAGAGAGCTGTATT
CATACAGTCAATTTTTGATTGAACGGCTTGAACGGAAAGCAGAAAAATGGACAGGTTTGATCCAGCCATTGATTTACGGG
CTAACAGCGGCGATGATATTAATTCTTTATTTGTCTATGCTTTTGCCAATGTATCAAATGATGAACCAGTTATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(216-336)

Type II secretion system (T2SS), protein F (T2SSF)

(16-135)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Bacillus subtilis subsp. subtilis str. 168

57.276

93.895

0.538

Multiple sequence alignment