Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   U3P88_RS05320 Genome accession   NZ_CP140297
Coordinates   1211195..1212265 (-) Length   356 a.a.
NCBI ID   WP_060674614.1    Uniprot ID   -
Organism   Bacillus velezensis strain UMAF6639     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1206195..1217265
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U3P88_RS05270 (U3P88_05270) - 1206255..1206839 (-) 585 WP_061860711.1 signal peptidase I -
  U3P88_RS05275 (U3P88_05275) tapA 1206811..1207482 (-) 672 WP_060674605.1 amyloid fiber anchoring/assembly protein TapA -
  U3P88_RS05280 (U3P88_05280) - 1207741..1208070 (+) 330 WP_060674607.1 DUF3889 domain-containing protein -
  U3P88_RS05285 (U3P88_05285) - 1208110..1208289 (-) 180 WP_003153093.1 YqzE family protein -
  U3P88_RS05290 (U3P88_05290) comGG 1208346..1208723 (-) 378 WP_015417814.1 competence type IV pilus minor pilin ComGG Machinery gene
  U3P88_RS05295 (U3P88_05295) comGF 1208724..1209119 (-) 396 WP_060674609.1 competence type IV pilus minor pilin ComGF -
  U3P88_RS05300 (U3P88_05300) comGE 1209133..1209447 (-) 315 WP_060674611.1 competence type IV pilus minor pilin ComGE -
  U3P88_RS05305 (U3P88_05305) comGD 1209431..1209868 (-) 438 WP_012117983.1 competence type IV pilus minor pilin ComGD Machinery gene
  U3P88_RS05310 (U3P88_05310) comGC 1209858..1210124 (-) 267 WP_060674857.1 competence type IV pilus major pilin ComGC Machinery gene
  U3P88_RS05315 (U3P88_05315) comGB 1210171..1211208 (-) 1038 WP_060674612.1 competence type IV pilus assembly protein ComGB Machinery gene
  U3P88_RS05320 (U3P88_05320) comGA 1211195..1212265 (-) 1071 WP_060674614.1 competence type IV pilus ATPase ComGA Machinery gene
  U3P88_RS05325 (U3P88_05325) - 1212458..1213408 (-) 951 WP_015417820.1 magnesium transporter CorA family protein -
  U3P88_RS05330 (U3P88_05330) - 1213554..1214855 (+) 1302 WP_012117986.1 hemolysin family protein -
  U3P88_RS05335 (U3P88_05335) - 1214893..1215723 (-) 831 WP_039254482.1 STAS domain-containing protein -
  U3P88_RS05345 (U3P88_05345) - 1215930..1216310 (-) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  U3P88_RS05350 (U3P88_05350) - 1216547..1216792 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40513.85 Da        Isoelectric Point: 9.4729

>NTDB_id=913524 U3P88_RS05320 WP_060674614.1 1211195..1212265(-) (comGA) [Bacillus velezensis strain UMAF6639]
MKNIETYSRNIINEAYTARASDIHIVPREKDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYAAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=913524 U3P88_RS05320 WP_060674614.1 1211195..1212265(-) (comGA) [Bacillus velezensis strain UMAF6639]
GTGAAAAATATTGAAACATATAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAAGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTTATTTCTCATTTTAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAACTGTCCTTATTTCGGGGGGCGGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTGTTTACCGGCCCCACCGGATCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAAAGGCATTTCAACCGGCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGTTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATGCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGGGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTCGGCGTGAACATGACAGAAATTGAACAAACCGTCATTGC
TATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGTAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

81.742

100

0.817
  pilF Thermus thermophilus HB27

38.177

98.596

0.376
  pilB Glaesserella parasuis strain SC1401

38.506

97.753

0.376